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R.M.A Adjid
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Development inactivated vaccine prototype of avian influenza (AI) H5N1 local isolate and its application at laboratory level Indriani, R; Dharmayanti, N.L.P.I; Syafriati, T; Wiyono, A; Adjid, R.M.A
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 4 (2005)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.851 KB) | DOI: 10.14334/jitv.v10i4.458

Abstract

A preliminary study related on vaccine safety and vaccination effectivity for controlling avian influenza (AI) subtype H5N1 was carried out at Virology Laboratorium, Indonesian Veteriner Institute, Bogor. A Prototype of inactivated vaccine was made using AI H5N1 local isolate (A/Chicken/West Java/67-2/2003). The vaccine was then tested for safety and protection in DOC of layers. Antibody response, protection and shedding virus challenge were observed in the experiment. Result showed that the vaccine was saved and protected against virulent viral challenge. Efective vaccination was achieved at 3 weeks chicken old started with low level of antibody. Antibody titre increased gradually and reached the top at 8 weeks post vaccination. Challenge test using AI virulent at the age of 4 and 8 weeks post vaccination showed that the vaccine gave high protection (90%). Viral shedding was not longer expressed than 7 days after challenge. It is concluded that this prototype is a satisfied AI vaccine in laboratory level.     Key Words: Vaccine, Avian Influenza, H5N1, HPAI
Monitoring of avian influenza cases based on the detection of viral antigen subtype H5N1 by immunohistochemical technique Damayanti, Rini; Dharmayanti, N.L.P.I; Indriani, R.; Wiyono, A.; Adjid, R.M.A
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 4 (2005)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.695 KB) | DOI: 10.14334/jitv.v10i4.1106

Abstract

Monitoring on the cases of Avian Influenza virus was conducted by detecting viral antigen subtype H5N1 usingimmunohistochemical technique. A total of 212 sampels of various avian tissues were collected from the Provinces of East Java(Districts of Madiun, Tulung Agung, Blitar and Kediri), West Java (Districts of Bogor, Bekasi, Cianjur and Sukabumi), Banten(Districts of Pandeglang and Tangerang) and DKI Jakarta. The sampels were collected four times i.e. June 2004, September2004, October 2004 and between January and February 2005. All sampels were stained using immunohistochemical technique.The antigen could be visualized clearly both in the intra-nuclear and intra-cytoplasmic areas of brain, comb, wattle, trachea,lung, heart, breast and thigh muscle, proventriculus, liver, spleen, kidney, intestine and ovary. A number of 39 of 212 cases(18.4%) have been catagorized as positives. The results show that monitoring of HPAI cases conducted in June and September2004 in the Provinces of West Java, Banten and East Java, none of the sampels were positive. However, monitoring of thedisease in September 2004 in the Province of Jakarta showed that AI virus antigen were detected in various organs of chickenfrom Jakarta. Furthermore, monitoring of the disease conducted between October 2004 and February 2005 revealed that AI virusantigen were also detected in chicken not only from Jakarta Provinces but also from Provinces of Banten and West Java. Basedon these results, it is concluded that between June and September 2004, HPAI infection were not found in areas where previousoutbreaks occured in the Provinces of Banten, West Java and East Java. However, the disease was spread in Jakarta Province inSeptember 2004 and subsequently to some districts in the Provinces of Banten and West Java. A part from this, anticipation ofdisease spread to currently AI-free areas should be considered as part of disease monitoring system.Key Words: Avian Influenza, H5N1, Monitoring, Immunohistochemistry, Poultry
Development inactivated vaccine prototype of avian influenza (AI) H5N1 local isolate and its application at laboratory level R Indriani; N.L.P.I Dharmayanti; T Syafriati; A Wiyono; R.M.A Adjid
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 4 (2005): DECEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.851 KB) | DOI: 10.14334/jitv.v10i4.458

Abstract

A preliminary study related on vaccine safety and vaccination effectivity for controlling avian influenza (AI) subtype H5N1 was carried out at Virology Laboratorium, Indonesian Veteriner Institute, Bogor. A Prototype of inactivated vaccine was made using AI H5N1 local isolate (A/Chicken/West Java/67-2/2003). The vaccine was then tested for safety and protection in DOC of layers. Antibody response, protection and shedding virus challenge were observed in the experiment. Result showed that the vaccine was saved and protected against virulent viral challenge. Efective vaccination was achieved at 3 weeks chicken old started with low level of antibody. Antibody titre increased gradually and reached the top at 8 weeks post vaccination. Challenge test using AI virulent at the age of 4 and 8 weeks post vaccination showed that the vaccine gave high protection (90%). Viral shedding was not longer expressed than 7 days after challenge. It is concluded that this prototype is a satisfied AI vaccine in laboratory level.     Key Words: Vaccine, Avian Influenza, H5N1, HPAI
Monitoring of avian influenza cases based on the detection of viral antigen subtype H5N1 by immunohistochemical technique Rini Damayanti; N.L.P.I Dharmayanti; R. Indriani; A. Wiyono; R.M.A Adjid
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 4 (2005): DECEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.695 KB) | DOI: 10.14334/jitv.v10i4.1106

Abstract

Monitoring on the cases of Avian Influenza virus was conducted by detecting viral antigen subtype H5N1 usingimmunohistochemical technique. A total of 212 sampels of various avian tissues were collected from the Provinces of East Java(Districts of Madiun, Tulung Agung, Blitar and Kediri), West Java (Districts of Bogor, Bekasi, Cianjur and Sukabumi), Banten(Districts of Pandeglang and Tangerang) and DKI Jakarta. The sampels were collected four times i.e. June 2004, September2004, October 2004 and between January and February 2005. All sampels were stained using immunohistochemical technique.The antigen could be visualized clearly both in the intra-nuclear and intra-cytoplasmic areas of brain, comb, wattle, trachea,lung, heart, breast and thigh muscle, proventriculus, liver, spleen, kidney, intestine and ovary. A number of 39 of 212 cases(18.4%) have been catagorized as positives. The results show that monitoring of HPAI cases conducted in June and September2004 in the Provinces of West Java, Banten and East Java, none of the sampels were positive. However, monitoring of thedisease in September 2004 in the Province of Jakarta showed that AI virus antigen were detected in various organs of chickenfrom Jakarta. Furthermore, monitoring of the disease conducted between October 2004 and February 2005 revealed that AI virusantigen were also detected in chicken not only from Jakarta Provinces but also from Provinces of Banten and West Java. Basedon these results, it is concluded that between June and September 2004, HPAI infection were not found in areas where previousoutbreaks occured in the Provinces of Banten, West Java and East Java. However, the disease was spread in Jakarta Province inSeptember 2004 and subsequently to some districts in the Provinces of Banten and West Java. A part from this, anticipation ofdisease spread to currently AI-free areas should be considered as part of disease monitoring system.Key Words: Avian Influenza, H5N1, Monitoring, Immunohistochemistry, Poultry
Co-Authors A Wiyono A. Wiyono N.L.P.I Dharmayanti R Indriani R. Indriani Sembiring, Rinawati T Syafriati