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Indonesian avian influenza viruses character in second wave epidemic Dharmayanti, N.L.P.I.; Indriani, R; Damayanti, R; Wiyono, A; Adjid, R.M.A.
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 3 (2005)
Publisher : Indonesian Animal Sciences Society

Second wave of epidemic avian influenza occurred from December 2004 until April 2005. In March 2005, the disease had infected some districts in South Sulawesi such as Wajo and Sopeng. More than 21 field isolates have been collected and identified as avian influenza virus subtype H5N1. In this study further characterized was undertaken for 14 isolates of avian influenza using Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and sequencing in region of HA1 gene. It was then followed by genetic analysis to identify the mutation and phylogenetic relationship of the isolates. The study indicates that the Indonesia isolates collected in second wave epidemic are generally having a different group to the isolates group in 2003 and 2004. There is point mutation in the nucleotide sequence of the isolate collected at August 2004-March 2005, that is the replacement of adenine by guanine in the position of 195. Â Â Key Words: Avian Influenza Virus, Second Epidemic Wave, Mutation

Molecular characterization of Indonesia avian influenza virus Dharmayanti, N.L.P.I.; Damayanti, R; Indriani, R; Wiyono, A; Adjid, R.M.A.
Indonesian Journal of Animal and Veterinary Sciences Vol 10, No 2 (2005)
Publisher : Indonesian Animal Sciences Society

Avian influenza outbreaks in poultry have been reported in Java island since August 2003. A total of 14 isolates of avian influenza virus has been isolated from October 2003 to October 2004. The viruses have been identified as HPAI H5N1 subtype. All of them were characterized further at genetic level and also for their pathogenicity. Phylogenetic analysis showed all of the avian influenza virus isolates were closely related to avian influenza virus from China (A/Duck/China/E319-2/03(H5N1). Molecular basis of pathogenicity in HA cleavage site indicated that the isolates of avian influenza virus have multiple basic amino acid (B-X-B-R) indicating that all of the isolates representing virulent avian influenza virus (highly pathogenic avian influenza virus). Â Â Key Words: Avian Influenza Virus, Molecular Characterization, Poultry, Indonesia

Protection of avian influenza (AI) vaccines for poultry against infection of field isolates A/Chicken/West Java/Smi-Pat/2006 and A/Chicken/West Java/Smi-Mae/2008 under laboratory condition Indriani, Risa; Dharmayanti, N.l.p.I.; Adjid, R.M.A.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 2 (2011)
Publisher : Indonesian Animal Sciences Society

The aim of this research was to study level of protection of avian influenza (AI) commercial vaccines available in Indonesia (subtipe H5N1, H5N2 and H5N9) against infection of HPAI field isolates of A/Chicken/West Java/Smi-Pat/2006 and A/Chicken/West Java/Smi-Mae/2008. There were 7 commercial vaccines used in this study, the each vaccines were injected in to 3 weeks old of layer chichickenen intramuscularly. At 3 weeks after vaccination, ten chichickenens from each group were challenged separately with the A/Chicken/West Java/Smi-Pat/2006 and A/Chicken/West Java/Smi-Mae/2008 isolates intranasaly with dose 106 ELD50 per 0,1 ml per chicken. Ten unvaccinated chicken were included in the challenge test as control. The study demonstrate that the AI vaccines with subtipe H5N1 protected chicken (100%) against virus of A/Chicken/West Java/Smi-Pat/2006 and 90-100% against virus A/Chicken/West Java/Smi-Mae/2008. Viral shedding were not seen by 2 days post challenge. The AI vaccines with subtipe H5N2 protected chicken at 20-30% against virus of A/Chicken/West Java/Smi-Pat/2006 and protected chicken at 70-100% against virus of A/Chicken/West Java/Smi-Mae/2008. Viral shedding still detected at 8 days post challenge. The AI vaccines AI with subtipe H5N9 did not protect chicken (0%) against virus A/Chicken/West Java/Smi-Pat/2006 and protected chicken at 50% against virus A/Chicken/West Java/Smi-Mae/2008. Viral shedding still detected by 8 days post challenge. This study concluded that AI vaccines with subtipe H5N1 are better than other AI subtipe vaccines in preventing HPAI virus A/Chicken/West Java/Smi-Pat/2006 dan A/Chicken/West Java/Smi-Mae/2008 infections under laboratory condition. Key Words: Avian Influenza, Vaccine, Poultry, Chicken

Potensi Virus A/Ck/West Java/PWT-Wij/2006 Sebagai Vaksin Indriani, R.; Dharmayanti, NLP; Adjid, R.M.A.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTPotential virus A/Ck/West Java/PWT-Wij/06 for Vaccin. Vaccination program for controllingavian influenza (AI) virus infection in poultry have been emerged during the past 5 years inIndonesia. However, due to the mutation character of this virus the available vaccines were nolonger effective. Therefore a new local isolate of avian influenza virus A/Ck/West Java/Pwt-Wij/2006 was studied. The virus, formulated as inactive vaccine, was injected in to 3 weeks oldof layer chickens intramuscularly. At 3 weeks after vaccination, vaccinated chickens werechallenged against seven local isolates of HPAI H5N1 intranasaly. Unvaccinated chickenswere included in the challenge test as control. Results showed that the vaccine produced100% protection against A/Ck/West Java/Pwt-Wij/2006 (homologous), A/Ck/West Java/1067/2003, A/Ck/West Java/Hj-18/2007, and A/Ck/Payakumbuh/BPPVRII/2007; produced 90%protection against A/Ck/BB149/5/2007, and 80% protection against A/Ck/West Java/Hamd/2006 isolates. The vaccine also stoppped viral shedding by day 5 to 7 after challange. Thisstudy indicate that the new local isolate of avian influenza A/Ck/West Java/Pwt-Wij/2006 hasgood potency as vaccine with broad spectrum against a range of AI viruses available inIndonesia.Key words: avian influenza, HPAI, H5N1,vaccine, poultry.

POTENSI VIRUS A/CK/WEST JAVA/PWT-WIJ/2006 SEBAGAI VAKSIN Indriani, R.; Dharmayanti, NLP; Adjid, R.M.A.; Darminto, Darminto
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3117

ABSTRACTPotential virus A/Ck/West Java/PWT-Wij/06 for Vaccin. Vaccination program for controllingavian influenza (AI) virus infection in poultry have been emerged during the past 5 years inIndonesia. However, due to the mutation character of this virus the available vaccines were nolonger effective. Therefore a new local isolate of avian influenza virus A/Ck/West Java/Pwt-Wij/2006 was studied. The virus, formulated as inactive vaccine, was injected in to 3 weeks oldof layer chickens intramuscularly. At 3 weeks after vaccination, vaccinated chickens werechallenged against seven local isolates of HPAI H5N1 intranasaly. Unvaccinated chickenswere included in the challenge test as control. Results showed that the vaccine produced100% protection against A/Ck/West Java/Pwt-Wij/2006 (homologous), A/Ck/West Java/1067/2003, A/Ck/West Java/Hj-18/2007, and A/Ck/Payakumbuh/BPPVRII/2007; produced 90%protection against A/Ck/BB149/5/2007, and 80% protection against A/Ck/West Java/Hamd/2006 isolates. The vaccine also stoppped viral shedding by day 5 to 7 after challange. Thisstudy indicate that the new local isolate of avian influenza A/Ck/West Java/Pwt-Wij/2006 hasgood potency as vaccine with broad spectrum against a range of AI viruses available inIndonesia.Key words: avian influenza, HPAI, H5N1,vaccine, poultry.

Indonesian avian influenza viruses character in second wave epidemic N.L.P.I. Dharmayanti; R Indriani; R Damayanti; A Wiyono; R.M.A. Adjid
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 3 (2005): SEPTEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Second wave of epidemic avian influenza occurred from December 2004 until April 2005. In March 2005, the disease had infected some districts in South Sulawesi such as Wajo and Sopeng. More than 21 field isolates have been collected and identified as avian influenza virus subtype H5N1. In this study further characterized was undertaken for 14 isolates of avian influenza using Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and sequencing in region of HA1 gene. It was then followed by genetic analysis to identify the mutation and phylogenetic relationship of the isolates. The study indicates that the Indonesia isolates collected in second wave epidemic are generally having a different group to the isolates group in 2003 and 2004. There is point mutation in the nucleotide sequence of the isolate collected at August 2004-March 2005, that is the replacement of adenine by guanine in the position of 195. Key Words: Avian Influenza Virus, Second Epidemic Wave, Mutation

Molecular characterization of Indonesia avian influenza virus N.L.P.I. Dharmayanti; R Damayanti; R Indriani; A Wiyono; R.M.A. Adjid
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 2 (2005): JUNE 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Avian influenza outbreaks in poultry have been reported in Java island since August 2003. A total of 14 isolates of avian influenza virus has been isolated from October 2003 to October 2004. The viruses have been identified as HPAI H5N1 subtype. All of them were characterized further at genetic level and also for their pathogenicity. Phylogenetic analysis showed all of the avian influenza virus isolates were closely related to avian influenza virus from China (A/Duck/China/E319-2/03(H5N1). Molecular basis of pathogenicity in HA cleavage site indicated that the isolates of avian influenza virus have multiple basic amino acid (B-X-B-R) indicating that all of the isolates representing virulent avian influenza virus (highly pathogenic avian influenza virus). Key Words: Avian Influenza Virus, Molecular Characterization, Poultry, Indonesia

Protection of avian influenza (AI) vaccines for poultry against infection of field isolates A/Chicken/West Java/Smi-Pat/2006 and A/Chicken/West Java/Smi-Mae/2008 under laboratory condition Risa Indriani; N.l.p.I. Dharmayanti; R.M.A. Adjid
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 2 (2011): JUNE 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The aim of this research was to study level of protection of avian influenza (AI) commercial vaccines available in Indonesia (subtipe H5N1, H5N2 and H5N9) against infection of HPAI field isolates of A/Chicken/West Java/Smi-Pat/2006 and A/Chicken/West Java/Smi-Mae/2008. There were 7 commercial vaccines used in this study, the each vaccines were injected in to 3 weeks old of layer chichickenen intramuscularly. At 3 weeks after vaccination, ten chichickenens from each group were challenged separately with the A/Chicken/West Java/Smi-Pat/2006 and A/Chicken/West Java/Smi-Mae/2008 isolates intranasaly with dose 106 ELD50 per 0,1 ml per chicken. Ten unvaccinated chicken were included in the challenge test as control. The study demonstrate that the AI vaccines with subtipe H5N1 protected chicken (100%) against virus of A/Chicken/West Java/Smi-Pat/2006 and 90-100% against virus A/Chicken/West Java/Smi-Mae/2008. Viral shedding were not seen by 2 days post challenge. The AI vaccines with subtipe H5N2 protected chicken at 20-30% against virus of A/Chicken/West Java/Smi-Pat/2006 and protected chicken at 70-100% against virus of A/Chicken/West Java/Smi-Mae/2008. Viral shedding still detected at 8 days post challenge. The AI vaccines AI with subtipe H5N9 did not protect chicken (0%) against virus A/Chicken/West Java/Smi-Pat/2006 and protected chicken at 50% against virus A/Chicken/West Java/Smi-Mae/2008. Viral shedding still detected by 8 days post challenge. This study concluded that AI vaccines with subtipe H5N1 are better than other AI subtipe vaccines in preventing HPAI virus A/Chicken/West Java/Smi-Pat/2006 dan A/Chicken/West Java/Smi-Mae/2008 infections under laboratory condition. Key Words: Avian Influenza, Vaccine, Poultry, Chicken

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