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All Journal Jurnal Veteriner Jurnal Ilmiah Fillia Cendekia Jurnal Nasional Teknik Elektro dan Teknologi Informasi Jurnal Kedokteran Hewan
Agung Budiyanto
Bagian Reproduksi dan Kebidanan, Fakultas Kedokteran Hewan, Universitas Gajah Mada (UGM)
Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Energy Engineering Immunology & microbiology Medicine & Pharmacology Veterinary Other

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 1 
Pelacakan Protein Wingless-Type MMTV Integration Site Family Member-4 pada Uterus Mencit (DETECTION WINGLESS-TYPE MMTV INTEGRATION SITE FAMILY MEMBER-4 PROTEIN OF MOUSE UTERUS) Agung Janika Sitasiwi; Wayan Tunas Artama; Agung Budiyanto; Edy Dharmana
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.168 KB)

Abstract

The aims of this study was to detect the expression of Wingless-type MMTV integration site familymember 4 (Wnt4) protein in the uterus of Swiss Webster mice. Laboratory animals that were used areadult Swiss Webster mice weighing 25-30 grams. Mice were kept and mated in a controlled laboratoryconditions. Pregnancy was determined by the presence of vaginal plug in female mice after breeding.Protein was isolated from the uterus at seven days of gestation. Immunoblotting was performed usingChemiluminescent Western Blot kit. The primary antibody used was anti Wnt-4 antibody with a 1: 1000dilution. The results showed protein bands with molecular weights ranging from 40 kDa showed a positivereaction to the primary antibody that were used so that it can be concluded that the protein is Wnt4protein.
UJI ORGANOLEPTIK NUGGET DAGING DADA ITIK AFKIR DENGAN JENISDAN DOSIS TEPUNG YANG BERBEDA Nurina Rahmawati; Agung Budiyanto
Jurnal Ilmiah Fillia Cendekia Vol 2 No 1 (2017): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

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Abstract

One of the resilience and diversification program of food were supplying and using local meat. Duck’s meat was soource of highly animal protein, but less of idea to process that meats. Nuggets was the produst processed meat that well liked many people. So expcted an be increase the economic of society. This research conducted at the campus of the Islamic University Kadiri on 29 april 2015 and was herped by 30 panelist. The purpose of this study to determine the species and levels or the different flour in nuggets ducks so that consumers prefer. The data obtained were analized using a completely randomized design analysis and if there is a noticeable difference then proceed to test LSD. The result showed that the species and levels or the different flour in nuggets ducks gave highly significant (P<0,01) for organoleptic tests include color, aroma, taste and texture. The conclusion of this research was the panelis liked the use of 20% level of flour in nuggets ducks
Studi Teknis Genset Termodifikasi Menggunakan Gas Alam dengan Variasi Tekanan Masukan Faiz Husnayain; Agung Budiyanto; Fauzan Hanif Jufri; I Made Ardita Y.
Jurnal Nasional Teknik Elektro dan Teknologi Informasi Vol 9 No 3: Agustus 2020
Publisher : Departemen Teknik Elektro dan Teknologi Informasi, Fakultas Teknik, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1267.343 KB) | DOI: 10.22146/.v9i3.393

Abstract

Every year Indonesia experiences an increase in the number of electricity customers. However, there are still many areas that do not yet have electricity supply or have frequent disturbances. Therefore, we need a generator set as the main electricity supply or as a backup for these areas. Natural gas can be used as generator fuel because of its abundant availability and is more environmentally friendly. However, the availability of gasfueled generators in Indonesia is still very rare, so that additional equipment such as regulators, converter kits, and air mixing equipment for gas can be used on gasoline generators. Due to this background and its potential, testing is carried out to determine the performance of the modified generator set when using natural gas fuels. The method used is to compare engine performance by varying the value of the generator's input pressure, which is 0.5 and 0.03 bar. The results observed were the SFC value, power quality, exhaust gas temperature, and the noise level produced by the generator. The results of this test are that the voltage and frequency generated is still within the specified normal limits, the exhaust gas temperature and the noise level generated when the input pressure is 0.5 bar and 0.03 bar are relatively the same, and the SFC value generated when the input pressure is 0.5 bar is 5.7-25% smaller than the SFC value at 0.03 bar input pressure.
TINGKAT MATURASI IN VITRO PADA OOSIT SAPI SILANGAN SIMMENTAL PERANAKAN ONGOLE DAN LIMOUSIN PERANAKAN ONGOLE Hermilinda Parera; Agung Budiyanto
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.529 KB) | DOI: 10.21157/j.ked.hewan.v9i2.2821

Abstract

Penelitian ini bertujuan mengetahui tingkat maturasi oosit sapi Simmental Peranakan Ongole (SimPO) dan Limousin Peranakan Ongole (LimPO) secara in vitro. Oosit dari Ovarium sapi yang berasal dari rumah potong hewan (RPH), dikelompokkan berdasarkan jenis sapi PO (kontrol), SimPO dan LimPO. Oosit diaspirasi dari ovarium dengan syringe 5 ml dan jarum 18 G. Oosit dengan kualitas A dan B yang digunakan dalam penelitian ini. Oosit dikultur dalam media maturasi TCM 199 100 µl drop dan dilapisi minyak mineral, diinkubasi pada suhu 38,5° C, CO2 5% dan kelembaban 95% selama 24 jam. Tingkat maturasi in vitro ditentukan dengan pewarnaan aceto orcein 1% untuk melihat tahapan maturasi dengan adanya perubahan konfigurasi kromosom dan membran inti berupa germinal vesicle, germinal vesicle breakdown, metafase I, anafase/telofase I, dan metafase II. Hasil penelitian menunjukkan tingkat maturasi in vitro oosit yang mencapai metafase II dari oosit sapi PO lebih tinggi secara signifikan dibandingkan sapi SimPO dan LimPO.
TINGKAT MATURASI IN VITRO PADA OOSIT SAPI SILANGAN SIMMENTAL PERANAKAN ONGOLE DAN LIMOUSIN PERANAKAN ONGOLE Hermilinda Parera; Agung Budiyanto
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2821

Abstract

Penelitian ini bertujuan mengetahui tingkat maturasi oosit sapi Simmental Peranakan Ongole (SimPO) dan Limousin Peranakan Ongole (LimPO) secara in vitro. Oosit dari Ovarium sapi yang berasal dari rumah potong hewan (RPH), dikelompokkan berdasarkan jenis sapi PO (kontrol), SimPO dan LimPO. Oosit diaspirasi dari ovarium dengan syringe 5 ml dan jarum 18 G. Oosit dengan kualitas A dan B yang digunakan dalam penelitian ini. Oosit dikultur dalam media maturasi TCM 199 100 µl drop dan dilapisi minyak mineral, diinkubasi pada suhu 38,5° C, CO2 5% dan kelembaban 95% selama 24 jam. Tingkat maturasi in vitro ditentukan dengan pewarnaan aceto orcein 1% untuk melihat tahapan maturasi dengan adanya perubahan konfigurasi kromosom dan membran inti berupa germinal vesicle, germinal vesicle breakdown, metafase I, anafase/telofase I, dan metafase II. Hasil penelitian menunjukkan tingkat maturasi in vitro oosit yang mencapai metafase II dari oosit sapi PO lebih tinggi secara signifikan dibandingkan sapi SimPO dan LimPO.
Co-Authors Agung Janika Sitasiwi Edy Dharmana Fauzan Hanif Jufri Heni Pujiastuti Hermilinda Parera Husnayain, Faiz I Made Ardita Y. Nurina Rahmawati Wayan Tunas Artama