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All Journal Biopropal Industri JURNAL PENDIDIKAN TAMBUSAI Makara Journal of Science Manilkara: Journal of Bioscience
Ni Wayan Sri Agustini
Research Center for Biotechnology-LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Neuroscience Social Sciences Veterinary

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AKTIVITAS ANTIBAKTERI DAN IDENTIFIKASI SENYAWA KIMIA ASAM LEMAK DARI MIKROALGA Lyngbya sp. - (ANTIBACTERIAL ACTIVITY AND FATTY ACID COMPOUNDS IDENTIFICATION FROM MICROALGAE Lyngbya sp.) Agustini, Ni Wayan Sri; Kusmiati, Kusmiati; Handayani, D
Biopropal Industri Vol 8, No 2 (2017)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.452 KB)

Abstract

Fatty acid has biological activity to terminate or inhibit the growth of pathogenic bacteria. Lyngbya sp. is one of microalgae that producing fatty acid. This study aims to obtain a compound with antibacterial activity from Lyngbya sp. The extraction method used specifically to isolate fatty acid with dichloromethane solvents. The A, B and C extracts were tested its antibacterial activity using diffusion method with paper disc. The C extract which most active was fractionated using SiO2 column chromatography, dichlromethane-ethyl acetate (1:1). The result then tested against Staphylococcus aureus and Escherichia coli. Fraction that had antibacterial activity with highest inhibition zone was identified using Gas Chromatography Mass Spectrometry. The 16th fraction of the C extract had the highest antibacterial activity with inhibition zone of 30.30 mm. The identification of 16th fraction showed it was phthalic acid (bis(2-ethylhexyl) phatalate 1.2 benzene dicarboxylic acid) with retention time 19.73 minutes which classified as fatty acid.Keywords: antibacterial, chromatography, fatty acid, Lyngbya sp.ABSTRAKAsam lemak memiliki aktivitas biologis untuk menghentikan atau menghambat pertumbuhan bakteri patogen. Salah satu mikroalga penghasil asam lemak adalah Lyngbya sp. Penelitian ini bertujuan  untuk memperoleh senyawa kimia yang mempunyai aktivitas antibakteri dari mikroalga Lyngbya sp. Metode ekstraksi yang digunakan khusus untuk mengisolasi senyawa asam lemak dengan pelarut diklorometan. Ekstrak yang diperoleh adalah ekstrak A, B dan C, masing-masing ekstrak lalu diuji aktivitas antibakterinya dengan metode difusi menggunakan kertas cakram. Setelah diketahui ekstrak C merupakan ekstrak teraktif, selanjutnya ekstrak tersebut difraksinasi menggunakan kromatografi kolom SiO2, dengan pelarut diklorometana-etil asetat (1:1). Fraksi yang diperoleh kemudian diuji aktivitas antibakterinya dan ditetapkan fraksi yang mempunyai daya hambat terhadap pertumbuhan Staphylococcus aureus dan Escherichia coli. Fraksi yang mempunyai aktivitas antibakteri dengan zona hambat tertinggi diidentifikasi dengan kromatografi gas-spektrometri massa. Fraksi 16 ekstrak C memberikan aktivitas antibakteri dengan zona hambat 30,30 mm. Identifikasi fraksi 16 dengan kromatografi gas-spektrometer massa menunjukkan bahwa kandungan senyawa yangbersifat antibakteri adalah asam ftalat (bis(2 etil heksil) ftalat 1,2 benzena asam karboksilat) dengan waktu retensi 19,73 menit yang merupakan golongan asam lemak.Kata kunci: antibakteri, asam lemak, kromatografi, Lyngbya sp.
Effect of Mg2+ and Fe2+ Concentrations in Culture Medium on CGF Formation from Microalgae Chlorella Pyrenoidosa Ink and Analysis of Amino Acids by Liquid Chromatography-Mass Spectrometry Kusmiati,; Rahmawati, Witry; Agustini, Ni Wayan Sri
Makara Journal of Science Vol. 19, No. 2
Publisher : UI Scholars Hub

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Abstract

Chlorella pyrenoidosa (C. pyrenoidosa) contains Chlorella Growth Factor (CGF), which consists of proteins and polysaccharides. CGF is located inside the nucleus of cells and is beneficial to humans as a food supplement, an immunity booster, and an antioxidant. CGF formation of C. pyrenoidosa is influenced by medium composition. C. pyrenoidosa INK was cultured in a modified basal medium (MBM) with various concentrations of Mg2+ (0.5, 1.0, and 1.5 g/L) and Fe2+ (3.5×10-4 and 5.0×10-4 g/L). The experiments were performed and analyzed under a completely randomized design using a 2-L bottle with three replications. The results showed that MBM with 1.0 g/L of Mg2+ and 3.5×10-4 g/L of Fe2+ yielded the optimal growth curve for C. pyrenoidosa. Analysis of protein content was carried out using the Lowry method with a spectrophotometer at λ=750 nm, and the obtained results were 0.0974 mg/mL (extract) and 6.4097 mg/ml (supernatant). Furthermore, analysis of glucose content was carried out using the phenol sulfate method (λ = 490 nm), and the obtained results were 49.331 ppm (extract) and 1566.911 ppm (supernatant). Analysis of amino acids in CGF using liquid chromatography-mass spectrometry (LC-MS) indicated the presence of tyrosine, proline, glutamate, alanine, valine, tryptopan, phenylalanine, methionine, and leucine-isoleucine.
Analisis Karakteristik Biokimia serta Pertumbuhan Spirulina platensis pada Campuran Limbah Cair Keju dan Media Zarrouk’s dengan Konsentrasi Berbeda Apriastini, Marsiti; Agustini, Ni Wayan Sri
MANILKARA: Journal of Bioscience Vol 3 No 1 (2024): Agustus
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v3i1.9664.2024

Abstract

Spirulina platensis is a filamentous, photosynthetic, multicellular blue-green microalga that thrives in various ecosystems. In addition to using Zarrouk's medium for the cultivation of S. platensis, wastewater can also serve as an alternative medium, as it contains nutrients such as nitrogen, phosphorus, and essential micronutrients for microalgal growth. The growth conditions of S. platensis are influenced by light, oxygen, and the pH of the medium. The aim of this study was to observe the growth and nutrient content of Spirulina platensis when cultivated in alternative growth media containing cheese liquid waste. This study utilized 15% cheese liquid waste in each treatment. The research involved several stages, including the cultivation of S. platensis to determine the growth pattern by measuring optical density (OD), protein content analysis using the Biuret method, and carbohydrate content analysis using the phenol-sulfuric acid method. The results showed that the lag phase of S. platensis growth occurred on day 2, during which the cells were still adapting to the wastewater. The exponential phase was observed on day 4, characterized by rapid and significant cell growth and development. In this phase, nutrients were sufficient to meet the needs of S. platensis cells, supported by favorable environmental factors. The highest protein and phycocyanin contents were observed in the mixture of cheese liquid waste and Zarrouk's medium at 100% concentration, while the highest carbohydrate and fat contents were observed in the mixture of cheese liquid waste and Zarrouk's medium at 50% concentration. In conclusion, the growth of S. platensis was most optimal in the mixture of cheese liquid waste and Zarrouk's medium at a 50% concentration.
Problematika dan Upaya Penyesuaian Perubahan Kurikulum Merdeka bagi Guru dalam Implementasi Manajemen Berbasis Sekolah Aridani, Ni Putu Intan; Prascitadewi, Ni Kadek; Agustini, Ni Wayan Sri; Sulastri, Ni Luh Resa; Swastika, I Kadek; Werang, Basilius Redan; Kusumawardani, Dewa Ayu Novi
Jurnal Pendidikan Tambusai Vol. 10 No. 1 (2026)
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jptam.v10i1.37896

Abstract

Kurikulum yang terus menerus berubah saat ini menyebabkan guru mengalami kesulitan dalam menerapkan pembelajaran berdasarkan kurikulum tersebut. Guru masih belajar kembali untuk bisa memahami Projek Profil Pelajar Pancasila (P5), menyusun perangkat pembelajaran, memanfaatkan teknologi digital dalam pembelajaran, dan guru harus melakukan penyesuaian dalam merancang tujuan pembelajaran sesuai kurikulum merdeka. Penelitian ini bertujuan untuk mengidentifikasi dan menganalisis berbagai problematika yang dihadapi guru dalam menyesuaikan diri dengan perubahan kurikulum, khususnya kurikulum merdeka dalam konteks manajemen berbasis sekolah. Metode yang digunakan dalam memperoleh data yakni pendekatan deskriptif kualitatif melalui teknik pengumpulan data berupa wawancara bersama salah satu guru di SD Negeri 2 Banjar Tegal. Hasil pembahasan menunjukkan bahwa guru mengalami beberapa kendala dalam menghadapi perubahan kurikulum, seperti penyesuaian terhadap program Projek Penguatan Profil Pelajar Pancasila (P5), penyesuaian dalam merancang rencana pembelajaran mendalam (RPM), penggunaan papan interaktif digital, dan merancang tujuan pembelajaran. Kesimpulan dari penelitian ini adalah bahwa keberhasilan implementasi Kurikulum Merdeka dalam kerangka manajemen berbasis sekolah sangat dipengaruhi oleh berbagai faktor, yaitu kesiapan guru dalam melakukan penyesuaian, dukungan kelembagaan, serta adanya program pelatihan dan pendampingan yang sistematis.
Co-Authors Apriastini, Marsiti Aridani, Ni Putu Intan Basilius Redan Werang D Handayani, D I Kadek Swastika Kusmiati Kusmiati Kusmiati, Kusumawardani, Dewa Ayu Novi Prascitadewi, Ni Kadek Rahmawati, Witry Sulastri, Ni Luh Resa