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Analisis Karakteristik Biokimia serta Pertumbuhan Spirulina platensis pada Campuran Limbah Cair Keju dan Media Zarrouk’s dengan Konsentrasi Berbeda Apriastini, Marsiti; Agustini, Ni Wayan Sri
MANILKARA: Journal of Bioscience Vol 3 No 1 (2024): Agustus
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v3i1.9664.2024

Abstract

Spirulina platensis is a filamentous, photosynthetic, multicellular blue-green microalga that thrives in various ecosystems. In addition to using Zarrouk's medium for the cultivation of S. platensis, wastewater can also serve as an alternative medium, as it contains nutrients such as nitrogen, phosphorus, and essential micronutrients for microalgal growth. The growth conditions of S. platensis are influenced by light, oxygen, and the pH of the medium. The aim of this study was to observe the growth and nutrient content of Spirulina platensis when cultivated in alternative growth media containing cheese liquid waste. This study utilized 15% cheese liquid waste in each treatment. The research involved several stages, including the cultivation of S. platensis to determine the growth pattern by measuring optical density (OD), protein content analysis using the Biuret method, and carbohydrate content analysis using the phenol-sulfuric acid method. The results showed that the lag phase of S. platensis growth occurred on day 2, during which the cells were still adapting to the wastewater. The exponential phase was observed on day 4, characterized by rapid and significant cell growth and development. In this phase, nutrients were sufficient to meet the needs of S. platensis cells, supported by favorable environmental factors. The highest protein and phycocyanin contents were observed in the mixture of cheese liquid waste and Zarrouk's medium at 100% concentration, while the highest carbohydrate and fat contents were observed in the mixture of cheese liquid waste and Zarrouk's medium at 50% concentration. In conclusion, the growth of S. platensis was most optimal in the mixture of cheese liquid waste and Zarrouk's medium at a 50% concentration.
Phycocyanin production from Galdieria sulphuraria 009 in palm oil mill effluent: growth, extraction, and antioxidant activity: Produksi fikosianin dari Galdieria sulphuraria 009 dalam limbah cair pabrik kelapa sawit: pertumbuhan, ekstraksi, dan aktivitas antioksidan Rahman, Delicia Yunita; Praharyawan, Swastika; Apriastini, Marsiti; Nurcahyani, Puji Rahmawati; Nafisyah, Ayu Lana; Fatriasari, Widya; Amrullah, Apip; Farobie, Obie
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 28 No 5 (2025): Jurnal Pengolahan Hasil Perikanan Indonesia 28(5)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v28i5.63115

Abstract

Palm oil mill effluent (POME), a major byproduct of the palm oil industry in Indonesia, is generated in large volumes and poses environmental risks due to its high organic content> Microalgae offer a promising approach to reduce this waste while simultaneously producing value-added biomass products. This study aimed to determine the optimal POME concentration for microalgal growth of G. sulphuraria 009, to evaluate phycocyanin yield, and to assess its antioxidant activity. This study was initiated with a preliminary screening using 5–50% POME to identify optimal microalgal growth conditions; cultivation in bioreactors with selected concentrations (2.5%, 5.0%, and 7.5%) to evaluate growth performance and chemical yields; and analysis of antioxidant activity and pigment content in both fresh and residual biomass. The preliminary stage revealed 5% POME as the upper threshold for growth, with 2.5% supporting optimal biomass comparable to control (Allen pH 2). Higher POME levels inhibited growth due to light attenuation and ammoniacal nitrogen toxicity. 2.5% POME recorded the highest phycocyanin yield per liter, while 7.5% POME yielded the highest antioxidant activity, likely due to oxidative stress. Antioxidant assays confirmed significant antioxidant activity in all phycocyanin extracts, with the highest activity in 7.5% POME, likely due to oxidative stress. Carotenoid and chlorophyll contents were evaluated in both fresh and residual biomass. Carotenoids were more abundant in fresh biomass, while chlorophyll -A was higher in residual biomass post-extraction, emphasizing the importance of extraction techniques in bioactive compound recovery. This study highlights G. sulphuraria 009 as a viable source of phycocyanin in POME-based cultivation, offering insights into industrial wastewater valorization and sustainable bioproducts.