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All Journal Prosiding Seminar Biologi Biopropal Industri Proceeding Biology Education Conference Makara Journal of Science Manilkara: Journal of Bioscience
Ni Wayan Sri Agustini
Research Center for Biotechnology-LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Veterinary

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Journal : Proceeding Biology Education Conference

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POTENSI ASAM LEMAK DARI MIKROALGA Nannochloropsis sp SEBAGAI ANTIOKSIDAN DAN ANTIBAKTERI Agustini, Ni Wayan Sri
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Abstract- In Indonesia, the use of microalgae is still lacking, therefore, the antibacterial and antioxidant activity of the extract fatty acids from microalgae Nannochloropsis sp. Nannochloropsis sp biomass. extracted by soxhletasi with petroleum ether and ethanol, whereas the fatty acid extraction method by Indonesian National Standard. Antibacterial activity by disc diffusion method and antioxidant activity using the method of reduction of free radicals (DPPH). The results showed that the petroleum ether extract showed antibacterial and antioxidant activity better than the ethanol extract. The results of the identification of fatty acids in petroleum ether extract was dodecanoic acid (18.88%), while the ethanol extract was hexadecanoic acid (14.02%). Keywords: antioxidant, antibacterial, fatty acid, Nannochloropsis sp
AKTIVITAS ANTIOKSIDAN DAN UJI TOKSISITAS HAYATI PIGMEN FIKOBILIPROTEIN DARI EKSTRAK Spirulina platensis Agustini, Ni Wayan Sri
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 9, No 1 (2012): Prosiding Seminar Nasional IX Biologi
Publisher : Universitas Sebelas Maret

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ABSTRAK   Pigmen fikobiliprotein diketahui dapat meningkatkan sistem kekebalan tubuh, sebagai pewarna alami untuk makanan, kosmetik dan obat-obatan.  Salah satu mikroalga penghasil pigmen fikobiliprotein adalah Spirulina platensis. Berdasarkan literatur, diketahui bahwa fikobiliprotein dapat meredam radikal bebas dari 2,2’-azobis(2-amidinopropane)dihydroxychloride (AAPH) dan mencegah inisiasi reaksi radikal berantai.  Pigmen fikobiliprotein diekstraksi dari biomassa Spirulina platensis dengan menggunakan larutan kalsium klorida 1%. Ekstrak yang diperoleh diuji aktivitas antioksidan secara peredaman radikal DPPH dan uji aktivitas biologi secara BSLT. Hasil penelitian menunjukkan bahwa ada efek peredaman radikal bebas dari pigmen fikobiliprotein dan ada aktivitas biologi dengan menghitung tingkat kematian larva Artemia salina Leach. Hasil tersebut dapat dilihat dari nilai IC50 sebesar 96,57µg/ml dan nilai LC50 sebesar 174,66µg/ml.   Kata Kunci: Antioksidan, toksisitas hayati, fikobiliprotein, antioksidan, Spirulina platensis.
Co-Authors Apriastini, Marsiti D Handayani, D Kusmiati Kusmiati Kusmiati, Rahmawati, Witry