Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal Jurnal Mandala Pharmacon Indonesia 3BIO: Journal of Biological Science, Technology and Management
Kurniati, Dwi Elfira
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Immunology & microbiology Medicine & Pharmacology Neuroscience

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Optimization of PCR Primers for Detection of Extended-Spectrum Beta-Lactamase Targeting CTX-M and TEM Genes Kurniati, Dwi Elfira; Riani, Catur; Hardiyanti, Renny; Rachmawati, Dian
3BIO: Journal of Biological Science, Technology and Management Vol. 7 No. 1 (2025): Vol. 7 No. 1 (2025)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2025.7.1.5

Abstract

Extended-Spectrum Beta Lactamase (ESBL) is an enzyme that inhibit the activity of third-generation cephalosporin antibiotics. CTX-M and TEM are the genes encoding the ESBLs. The horizontal spread of these gene from one bacteria to another leads to increased bacterial resistance. Molecular-based bacterial identification methods such as Polymer Chain Reaction are methods that are currently used because they provide faster and more specific results. Therefore, in this study, a method for identifying bacteria that produce ESBL was created by targeting CTX-M and TEM genes. In this study, two pairs of primers were designed using in silico method, then the characteristics of the primers were analyzed. The primer annealing temperature was optimized using 55 and 60oC temperatures. The results of the in silico analysis showed that both pairs of primers met the ideal characteristics of a primer, as the Tm, %GC content, and secondary product values fulfilled the required criteria. Meanwhile, the results of the primer annealing temperature optimization indicated that the optimal temperature for the PCR method using both primers was 60°C.
Pemanfaatan Pewarna Alami dari Bunga Asoka (Ixora coccinea) pada Formulasi Lip Cream Nanda, Chintiya Vera; Kurniati, Dwi Elfira; Indriyanti, Niken
Jurnal Mandala Pharmacon Indonesia Vol. 10 No. 1 (2024): Jurnal Mandala Pharmacon Indonesia Special Issue for 18th Mulawarman Pharmaceu
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v10i1.534

Abstract

Bunga asoka (Ixora coccinea) mengandung pigmen untuk dimanfaatkan menjadi pewarna alami dalam bidang kosmetik. Tujuan dari  penelitian ini adalah meneliti manfaat ekstrak etanol dari bunga asoka yang menjadi pewarna alami terutama dalam produk lip cream. Studi dimulai melalui ekstrasi bunga asoka menggunakan pendekatan maserasi dimana pelarut etanolnya adalah 96% serta asam sitrat. Kemudian dilaksanakan pengoptimasian basis menggunakan lilin carnauba serta lilin candelilla, kemudian diformulasikan dengan ekstrak bunga asoka dan dievaluasi kualitasnya melalui uji organoleptik, pH, stabilitas sediaan, iritasi, daya lekat, viskositas, homogenitas, uji hedonik. Ekstraksi menghasilkan rendemen sebesar 27,1%. B3, B4, dan B5 adalah basis yang memenuhi kriteria lip cream dengan basis terbaik yaitu B3. Lip cream dengan konsentrasi ekstrak bunga asoka 2%, 4%, serta 6% memberikan hasil berupa warna masing-masing cokelat muda, cokelat sedikit merah, serta merah. Hasil evaluasi menunjukkan aroma khas cokelat, konsistensi semipadat, dan homogenitas yang sesuai dengan persyaratan lip cream. Namun, F3 menunjukkan ketidakstabilan dan tidak memenuhi persyaratan pH. Kesimpulan akhir menunjukkan bahwa F1 dan F2 merupakan formula yang lulus uji kriteria lip cream, sementara F3 adalah formula yang paling disukai. Kesimpulannya adalah hasil pengekstraksian etanol bunga asoka bisa dipergunakan menjadi pewarna alami dalam produk lip cream.
Co-Authors CATUR RIANI Dian Rachmawati Hardiyanti, Renny Indriyanti, Niken Nanda, Chintiya Vera