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Optimasi transplantasi menggunakan sel donor dari ikan gurame muda dan ikan nila triploid sebagai resipien Alimuddin .; M. Zairin Junior; Harton Arfah
Jurnal Ilmu Pertanian Indonesia Vol. 15 No. 3 (2010): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1375.475 KB)

Abstract

Testicular cell transplantation technology can be used in fish seed production engineering. In this study, optimization of transplantation using donor cells from young gouramy and triploid tilapia (3N) as recipient. Triploid tilapia is produced using heat shock method. The testes of male gouramy (body weight of 400- 850 g) was dissociated using 0.5% trypsin. Dissociated testicular cells was injected into the peritoneal cavity of tilapia larvae. Analysis of donor cell colonization was carried out using PCR method with DNA template that had been extracted from the gonad of 2-month-old tilapia. PCR was performed using specific primers for the growth hormone gene and (3-actin as an internal control of DNA loading. The results of nucleoli preparation showed that the success of triploidyzation was 88.5%. The gonad size of diploid (2N) and 3N recipient were relatively similar, while in not transplanted 3N tilapia was rudimentary. PCR results showed that the transplanted 3N tilapia has a DNA band of the same size with gouramy, while in control was not. This indicated that donor cells have been colonized in the gonads of recipient. The donor cell colonization in recipient 3N (78%) was higher than that of 2N (50%). Further research is required to determine the ability of donor cells differentiate into sperm and eggs in recipient gonad.
Fermentation quality and nutritive value of rice crop residue based silage ensiled with addition of epiphytic lactic acid bacteria B Santoso; B.Tj. Hariadi; Alimuddin .; D.Y. Seseray
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 1 (2011): MARCH 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.211 KB) | DOI: 10.14334/jitv.v16i1.628

Abstract

Silage is the feedstuff resulted from the preservation of forages through lactic acid fermentation. The aim of this study was to evaluate nutritive value, fermentation characteristics and nutrients digestibility of rice crop residue based silage ensiled with epiphytic lactic acid bacteria (LAB). The mixture of rice crop residue (RC), soybean curd residue (SC) and cassava waste (CW) in a 90: 5: 5 (on dry matter basis) ratio was used as silage material.  Three treatments silage were (A) RC + SC + CW as a control; (B) RC + SC + CW + LAB inoculums from rice crop residue; (C) RC + SC + CW + LAB inoculums from king grass.  Silage materials were packed into plastic silo (1.5 kg capacity) and stored for 30 days. The results showed that crude protein content in B and C silage was higher than that of silage A, but NDF content in silages B and C was lower than that of silage A.  Lactic acid concentration was higher (P < 0.01) in silage C compared to silage B and A, thus pH value of silage C was lower (P < 0.01) than silage B and A. Silage C had the highest Fleigh point than that of other silages. Dry matter and organic matter digestibilities were higher in silages B and C (P < 0.01) than that of control silage. It was concluded that the addition of LAB inoculums from king grass to rice crop residue based silage resulted a better fermentation quality compared to LAB inoculums from rice crop residue. Key Words: Silage, Rice Crop Residue, Lactic Acid, In Vitro
PENGARUH KUALITAS PELAKSANAAN PEMBELAJARAN, KETERSEDIAAN SARANA BELAJAR, DAN MOTIVASI BELAJAR TERHADAP PRESTASI BELAJAR IPS SISWA PADA UPT SDI 117 KEPULAUAN SELAYAR Alimuddin .
Jurnal Ilmiah Pena: Sains dan Ilmu Pendidikan Vol 13, No 2 (2022): Jurnal Ilmiah Pena Volume 13 Nomor 2 November 2022
Publisher : STKIP Pembangunan Indonesia Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54776/jip.v13i2.362

Abstract

This study aims to determine the effect of the quality of learning implementation on student social studies learning achievement at UPT SDI 117 Selayar Islands. 1) the influence of the availability of learning facilities on students' social studies learning achievement at UPT SDI 117 Selayar Islands. 2) the influence of student learning motivation on student social studies learning achievement at UPT SDI 117 Selayar Islands. 3) the influence of the quality of learning implementation, availability of learning facilities, and learning motivation simultaneously on student social studies learning achievement at UPT SDI 117 Selayar Islands. The type of research used is quantitative research. Quantitative research is a method used to answer research problems related to data in the form of numbers and statistical programs. The population in this study were all students of UPT SDI 117 Selayar Islands with a total sample of 17 people for class VI. Data collection techniques using questionnaires and documentation. The data analysis technique used descriptive analysis and multiple linear regression analysis. The results showed that 1) there was an influence between the quality of learning implementation on students' social studies learning achievement at UPT SDI 117. 2) there was an influence between the availability of learning facilities on students' social studies learning achievement at UPT SDI 117 Selayar islands according to the partial test which showed that 3) there is an influence of learning motivation on students' social studies learning achievement at UPT SDI 117 Selayar Islands. 4) there is an influence between the quality of learning implementation, availability of learning facilities, and learning motivation on students' social studies learning achievement at UPT SDI 117 Selayar Islands.Keywords: Learning, Learning Tools, Motivation, Achievement, Linear Regression