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All Journal Indian Journal of Forensic Medicine & Toxicology
May Naji Alkhanaq
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Public Health

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Prevalence of Cryptosporidium spp. among Patients with Diarrhea at Wasit Province/ Iraq May Naji Alkhanaq; Ghadeer Thamer
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17588

Abstract

Cryptosporidium is an important obligate enteric protozon parasite that infects humans and wild range of animals with morbidity and mortality especially amongimmuno-suppressed individuals[1]This study carried out in al-kut city /Iraq in two major hospital Al- kut hospital for gynecology ,obstetric and pediatric and al-Karamma teaching hospital which included collection of stool samples from diarrheal patients from October / 2020 – January / 2021 , (109) stool samples taken from patients with different ages to both gender examined by microscopic examination(Modified ziehl nelson) and multiplex PCR technique, the results showed the percentage of cryptosporidium spp. infection by using M.Z.N stain was 44(40.4%) while by using PCR technique was 38(39.6%) and 4(4.2%) for C.parvum and C.hominis respectively .The rate of infection is significantly associated with residence when using M.Z.N stain and PCR for C.parvum, also significant associate among those who >5 years old. water source for drinking were significant associated factors, other factors (gender, education level) Showed no significant association with infection, the present study aimed to detection of Cryptosporidium spp. and possible association between the infection and some risk factors by using microscopic examination and detecting DNA of Cryptosporidium parasite by multiplex PCR technique.
Use of Molecular Method to Detect Giardiasis in Different Animal in Al-Qadissiya Province – Iraq Ghaidaa Abass Jasim; May Naji Alkhanaq; Musafer H. Al_Ardi; Hanaa Salih Abd Ali Alrammah
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15752

Abstract

Giardiasis infection main causative agent of humans, companion animals, livestock and wild life is G. lamblia. Thus, a total of 300 fecal samples of cattle, sheep, goats suffered from diarrhea in slaughtering house in AL-Diwanyha province were collected. The multiplex PCR technique applied for G. lamblia detection as well as genotyping. The research findings revealed that 154 positive cases, their prevalence percentage were 35, 35 and 30 respectively. Giardia lamblia E, A, B and AB assemblages genotypes showed significant differences, indicating a potential for zoonotic spread under definite situations where humans living in close contact with livestock, therefore, More awareness urgently needed to eliminate these infections.
Co-Authors Ghadeer Thamer Ghaidaa Abass Jasim Hanaa Salih Abd Ali Alrammah Musafer H. Al_Ardi