Andriati Andriati
Balai Besar Pengkajian dan Pengembangan Teknologi Pertanian

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THE EXPRESSION OF TGF-1 AFTER LOW LEVEL LASER THERAPY IN INFLAMMATION ANIMAL MODEL Subadi, Imam; Wardhani, Indrayuni Lukitra; Andriati, Andriati
Folia Medica Indonesiana Vol. 53 No. 1 (2017): JANUARY - MARCH 2017
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.524 KB) | DOI: 10.20473/fmi.v53i1.5486

Abstract

Pain is the most common complaints in everyday practice. Pain is a clinical manifestation caused by stimuli due to potential tissue damage. Low level laser therapy (LLLT) has been used in reducing pain. One of indicator tissue healing is activation of transforming growth factor -b1(TGF-b1). Tissue healing will eliminate the pain. Do LLLT stimulate TGF-b1 expression? The objective of this study was to investigate the expression of TGF-b1 in low level laser therapy. Thirty male Wistar rats, body weight 250 – 300 gram, 3 month old, were divided into 3 groups, negative control (n=10), positive control (n=10) and treatment groups (n=10). The positive control and treatment groups were injected with Complete's Freund Adjuvant (CFA) 100 mL at left footpad. The treatment group treated with low level laser therapy Lasermed type 2100 wavelength 905 nm, out power 25-500 mW, dose 1 joule/cm2;5 times with interval 24 hours on left footpad. Samples were taken from the skin of footpad and immunohistochemical examination with monoclonal antibody anti- TGF-b1. Calculations carried out on brown reaction in macrophage cell. Data were analyzed using the Games Howell with SPSS 17. There were significant differences in the expression of TGF-b1 in treatment group (15.8±3.1) compared with the positive control group (3.9±1.1) and negative control group (4.6±2.1). There were no significant differences between expression TGF-b1 in positive control group and negative control group. In conclusion, low level laser therapy increased the expression of TGF-b1.
The Effects of Micronutirents on Growth and Yield of Lowland Rice Grown on Typic Dystrudept Soil Husnain, Husnain; Setyorini, Diah; Asmarhansyah, Asmarhansyah; Andriati, Andriati
JOURNAL OF TROPICAL SOILS Vol. 24 No. 1: January 2019
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2019.v24i1.1-10

Abstract

Fertilizers and fertilization technology have important roles in increasing crop production including lowland rice. In response to the issue, a field experiment was conducted to study the effects of micronutrients on growth and yield of lowland rice. The experiment was carried out at Cihideung Ilir Village, Dermaga Sub-district, Bogor Regency from November 2015 until March 2016. The experiment consisted of 8 treatments, namely control (without fertilization), NPK-standard, NPK-standard  + ¼ dosage of Hortigro Rice fertilizer, and ¾ NPK  + ¼  until 11/4 dosages of Hortigro Rice fertilizer as a source of macronutrients N, P, K and micronutrients of Mn, Cu, Zn, B, and Mo. The treatments were arranged in a Randomized Block Design with 3 replications. Parameters observed during the research were plant height, tiller number, and yield of grain and straw of INPARI-32 lowland rice as crop indicator. The  results  showed that among other treatments, the highest plant height was resulted from the application of ¾ NPK-standard + ¼ Hortigro Rice, i.e. around 124 cm at 60 days after planting. The highest harvesting dry weight of grain and straw were observed in NPK-standard + 1/4 of Hortigro Rice fertilizer treatment, i.e. around 9.0 Mg ha-1 and 25.9 Mg ha-1, respectively. This yield showed a significant difference with ¾ NPK-standard combined with ¾  -11/4 dosages of Hortigro Rice. The highest  dry weight of  milled grains was  resulted in the application of  ¾ NPK-standard + 1/2 Hortigro Rice, i.e. around 7.3 Mg ha-1, which is significantly different from that in ¾ NPK-standard combined with 1.0 - 11/4 dosages of Hortigro Rice treatments, while the highest dry weight of straw was provided by NPK-standard, i.e. around 14.0 Mg ha-1. The highest  dry weight of 1000 milled grains and ratio of  dry weight of milled grain to straw were provided by ¾ NPK-standard + 1/2 Hortigro Rice around 32.0 gram and  0.63, respectively. The results indicated that the application of micronutrient fertilizer of Hortigro Rice in combination with NPK fertilizers can potentially increase lowland rice productivity due to the micronutrients content of Mn, Cu, Zn, B, and Mo in it.