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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurasik (Jurnal Riset Sistem Informasi dan Teknik Informatika)
A Anggraini
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Agriculture, Biological Sciences & Forestry Computer Science & IT Decision Sciences, Operations Research & Management

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Kappa-Casein Genotypic Frequencies in Holstein-Friesian Dairy Cattle in West Java Province A Anggraini; C Sumantri; A Farajallah; E Andreas
Media Peternakan Vol. 33 No. 2 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.958 KB) | DOI: 10.5398/medpet.2010.33.2.61

Abstract

Kappa casein (ĸ-cn) gene as one of the four casein genes commonly has two variant alleles. B allele is positively correlated with milk proteins, so potentially be used as MAS to improve milk protein content. This study aimed to identify genetic variation of the ĸ-casein gene in Holstein-Friesian (HF) from several dairy regions in West Java. Blood samples were taken through jugular vein of lactating heifers and cows, from intensive managements under the Local Government Dairy Breeding Station - Cikole (BBPT Cikole-SP) (82 hds), Embryo Transfer Station (BET) (50 hds), and Eco Farm of Animal Science Faculty of Bogor Agricultural University (EcoFarm) (20 hds); as well as from semi-intensive managements of small dairy farmers under the supervision of the North Lembang Cooperative Unit (KPSBU Lembang) in two villages of Cilumber (98 hds) and Pasar Kemis (92 hds).  Blood samples were also taken via cocsigalis vein of AI (active and non active) HF bulls at the two National AI Stations in Lembang (25 hds) and Singosari (32 hds).  Identification of genetic variation was by applying PCR-RFLP method. Three genotypes were identified, namely AA, AB, and BB, resulting two alleles of A and B. Results entirely showed that the frequencies of the BB HF female were very low, with the range of 0%-6% (vs AA genotype= 10%-54% and AB genotype= 46%-85%), despite of the relatively high frequency of the B allele over the A allele (23%-48% vs. 52%-77%). The low frequency of the observed BB females might be due to the limited AI active BB bulls used for services by the two national AI stations (0%-4%).
GHRH|HaeIII Gene Polymorphism in Dairy and Beef Cattle at National Livestock Breeding Centers A O Rini; C Sumantri; A Anggraini
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1164.214 KB) | DOI: 10.5398/medpet.2013.36.3.185

Abstract

This study was aimed to identify polymorphism of growth hormone releasing hormone (GHRH) gene in 89 heads of Holstein-Friesian (HF) dairy cattle from Lembang Artificial Insemination Center/LAIC (17 bulls), Singosari Artificial Insemination Center/SAIC (32 bulls), and Cipelang Livestock Embryo Center/CLEC (40 cows); as well as in 4 breeds of female beef cattle from CLEC for comparison, providing Simmental (13 cows), Limousin (14 cows), Brahman (5 cows), and Angus (5 cows). This study used PCR-RFLP method by using HaeIII restriction enzyme. The enzyme cut the GHRH gene at nucleotides of GG|CC at the base positions of 118, 312, and 406 and produced 4 fragments of 118, 194, 94, and 45 bp respectively.  Genotyping the GHRH gene produced two types of allele, namely A (312, 94, and 45 bp) and B (194, 118, 94, and 45 bp). These two alleles resulted in three types of genotype, namely AA (312, 94, and 45 bp), AB (312, 194, 118, 94, and 45 bp), and BB (194, 118, 94, and 45 bp).  Frequency of the B allele was dominant to the A allele. Chi-Square analysis showed that all of HF dairy and beef cattle observed were in Hardy-Weinberg equilibrium (X2 < X2(0.05)). The highest heterozygosity value was 0.471 for HF bulls in LAIC, while the lowest one was for HF bulls in SAIC. Heterozygosity values in Simmental and Limousin cattles were higher than that of in HF cattle. The GHRH gene in HF and beef cattle was polymorphic, the exception was for Brahman with the only B allele. This result will improve the understanding of the polymorphism of GHRH gene in dairy and beef cattle.
Analisis Kualitas Layanan Website Pemerintahan Dispusip Kota Pekanbaru Dengan Metode E-Govqual Darmawan, Reza; Salisah, Febi Nur; Anggraini, A; Anwar, Tengku Khairil
Jurasik (Jurnal Riset Sistem Informasi dan Teknik Informatika) Vol 8, No 2 (2023): Edisi Agustus
Publisher : STIKOM Tunas Bangsa Pematangsiantar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30645/jurasik.v8i2.628

Abstract

The Pekanbaru City Office is a library in the field of information media dissemination using an E-Government-based website. This study aims to determine the results of service quality analysis on the Pekanbaru City Disputation website using the E-Govqual method which functions to measure the quality of E-Government services with 4 E-Govqual dimensions consisting of, ease of use, trust. , content and appearance of information, and citizen support. In interviews with 30 users, it was found that there were several problems regarding the website. By using E-Govqual the measurement of the information system will provide dimensions that are in accordance with the problem variables taken from interviews with these users. This study collected data with a questionnaire as a research instrument. The research questionnaire consisted of 24 statements with a total of 100 respondents. The results of this analysis conclude that there are 4 variables that are the top priority for improving the quality of website services, namely search (EF3), use of personal data (TR4), images, colors, graphics, animation and web size (CA1), and frequent questions filed (CS6). There are also 4 variables that have the highest satisfaction score that need to be maintained, namely the website address/url (EF2), access control (TR2), completeness of information (CA5), and knowledge and courtesy of employees (CS4). From the results obtained the average value of statistical analysis exceeds 3 of the four dimensions, this shows the need to improve website performance and the citizen support variable has the highest significant influence on community support on the Pekanbaru City Dispusip website.
Co-Authors A O Rini ACHMAD FARAJALLAH Anwar, Tengku Khairil C Sumantri Darmawan, Reza E Andreas Febi Nur Salisah, Febi Nur