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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Ternak Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences

C Sumantri

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Author-ID : 464956

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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The Role of Triglyceride Lipase, Fatty Acid Synthase and Fatty Acid Binding Protein Family Genes on Fat Metabolism of Broiler Chickens Ulupi, Niken; Sumantri, C
Indonesian Bulletin of Animal and Veterinary Sciences Vol 25, No 1 (2015)
Publisher : Indonesian Animal Sciences Society

Broiler chicken has high fat content, especially abdominal and subcutan fat which reduced carcass quality and efficiency of feed energy. Genetic approach could be potentially applied to reduce high abdominal and intramuscular fat in broiler chicken through the selection program at gene level related to fat metabolism. This paper describes the metabolism and biosynthesis of body fat and the role of its controlled genes. Fat synthesis is controlled by feed quality and metabolism and biosynthesis process occurred in liver. These processes are controlled by many family genes, but certain genes have dominant role in the process; those are triglyceride lipase genes, fatty acid synthase genes and fatty acid binding protein genes. Expression of fatty acid synthase genes has positive correlation with fat content in liver and intramuscular. Expression of fatty acid binding protein genes was related to the increased abdominal fat deposit. These genes are polymorphic, so that they can be used as a genetic marker in selection to optimize feed efficiency, to minimize abdominal fat and to increase economic value of broiler chicken. Key words: Fat, triglyceride lipase genes, fatty acid synthase genes, fatty acid binding protein genes

Pendugaan Jarak Genetik pada Itik Cihateup, Cirebon dan Mojosari A Muzani; B Brahmantiyo; C Sumantri; A Tapyadi
Media Peternakan Vol. 28 No. 3 (2005): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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A study on morphological body conformation of Cihateup, Cirebon and Mojosari ducks was carried out to determine the genetic distance and discriminant variables. The research was done at PT Dwi Mitra Daq, Gunung Sindur, Bogor, using 30 Cihateup, 30 Cirebon and 30 Mojosari ducks, from September to November 2004. Length of neck, wing, circumference of chest, deep chest, body weight, circumference of tarsometatarsus, length of chest, third digit, tarsometatarsus, tibia, femur and maxilla were measured to observe their body sizes to determine the genetic distance and discriminant variables. Data obtained were analyzed by using GLM (General Linear Model) and simple discriminant analysis with SAS package program version 6.12 and program MEGA2 to make the construction of tree fenograms. Cihateup ducks had larger body size (i.e. length of neck, wing, circumference of chest, deep chest, length of third digit and femur) than Cirebon and Mojosari ducks, but were smaller on length of tarsometatarsus than Mojosari ducks. Results from canonical analysis showed that the most discriminant variables were obtained from the length of third digit tarsometatarsus, tibia and femur. Cihateup ducks were mixed with Cirebon ducks (36,67%), Cirebon ducks were mixed with Cihateup ducks (43,33%) and Mojosari ducks (3,33%), Mojosari ducks were mixed with Cihateup ducks (3,33%) and Cirebon ducks (13,33%). Mahalanobis genetic distance showed that Cihateup and Cirebon ducks had a genetic distance of 0,880, Cihateup and Mojosari, and, Cirebon and Mojosari had 1,786. Key words : duck, morphologycal, genetic distance, body conformation

Estimasi Jarak Genetik antar Domba Garut Tipe Tangkas dengan Tipe Pedaging S S Mansjoer; T Kertanugraha; C Sumantri
Media Peternakan Vol. 30 No. 2 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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The objectives of the present study were to estimate the genetic distance and to determine the discriminant variables among fighting and meat types sheep of Garut (Garut sheep). A total of 413 heads of Garut sheep from five sub-populations were used in this study. Data were collected from the Regional Animal Research Center (BPPTD) as follows: 102 heads of common type sheep from Margawati (M), 69 heads from meat type sheep from Wanaraja (P), 72 heads of meat type from Sukawening (D), 81 heads of fighting sheep from Wanaraja (T) and 89 heads of fighting sheep from Sukawening (A). Data obtained were analyzed by simple discriminant with SAS package program version 7.0 and MEGA2 program to get the construction of phenogram tree. The results showed that in general fighting sheep had body weights and body measurements significantly higher (P < 0.05) than meat type sheep. However they were almost the same as common type sheep Margawati. The length and width of ears, tail width and chest width were the most discriminant variables to determine the differences of fighting type and meat type sheep. The highest similarities between individuals inside the group were obtained from Margawati common type sheep (71.57%), followed by Wanaraja meat type sheep (69.57%), Sukawening meat type sheep (69.44%), Wanaraja fighting type sheep (69.14%) and Sukawening fighting type sheep (59.55%). The Wanaraja fighting type sheep (T) had the closest genetic distance (1.16) to the Sukawening fighting type sheep (A), while the longest genetic distance (6.17) was between the Wanaraja meat type sheep and the Margawati common type sheep. The phenogram tree showed that the Margawati common type sheep was outside Wanaraja and Sukawening fighting type and meat type sheep, but the genetic distances were closer for the Wanaraja and Sukawening fighting type sheep. Key words: Garut sheep, genetic distance, body measurements

Keragaman Genetik DNA Mikrosatelit dan Hubungannya dengan Performa Bobot Badan pada Domba Lokal C Sumantri; A Farajallah; J F Salamena
Media Peternakan Vol. 31 No. 1 (2008): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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The objectives of this study were to evaluate polymorphism of microsatellite DNA through the investigation of microsatellite loci of CSSM18, ILST54 and IDVGA30 and its possible association with body weight of local sheeps. A total of 266 head of DNA samples was collected from 8 local sheep populations, i.e. Garut fighting type from Ciomas-Bogor (25); Garut meat type from Margawati-Garut (29); thin tail sheep (TTS) from Jonggol-Bogor (33); and fat tail sheep (FTSs) from Indramayu (40), Madura (34), Sumbawa (26), Rote (35) and Donggala (44). Genetic polymorphism among groups of local sheep was calculated in frequency of alleles and genotypes. Association between genotypes and body weight was calculated by general linear model (GLM). Results showed that three loci showed high polymorphisms. CSSM18 locus had 12 genotypes with the highest frequency for CC (46,15%) then for AC (25,64%); whilst frequency of the other 10 genotypes providing AA, AE, BB, BC, BD, BE, CD, CE, DD and EE were less than 7%. IDVGA30 locus had 9 genotypes with three highest frequencies for DD (39,10%), CC (37,97%) and BB (10,90%); while the other 6 genotypes were less than 6 %. ILST54 locus had 8 genotypes with three highest frequencies for AA (39,85%), BB (21,43%) and BC (18,05%) respectively. It could be verified that local sheep possessing typical genotypes of CE in CSSM18 locus and CD in IDVGA30 locus was apparently related with a higher body weight compared to another genotypes; on the contrary, those possessing DD genotypes in ILSTS54 was seemingly associated with a lower body weight. Key words: local sheep, microsatellite DNA, genotypes, body weight

Kappa-Casein Genotypic Frequencies in Holstein-Friesian Dairy Cattle in West Java Province A Anggraini; C Sumantri; A Farajallah; E Andreas
Media Peternakan Vol. 33 No. 2 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Kappa casein (ĸ-cn) gene as one of the four casein genes commonly has two variant alleles. B allele is positively correlated with milk proteins, so potentially be used as MAS to improve milk protein content. This study aimed to identify genetic variation of the ĸ-casein gene in Holstein-Friesian (HF) from several dairy regions in West Java. Blood samples were taken through jugular vein of lactating heifers and cows, from intensive managements under the Local Government Dairy Breeding Station - Cikole (BBPT Cikole-SP) (82 hds), Embryo Transfer Station (BET) (50 hds), and Eco Farm of Animal Science Faculty of Bogor Agricultural University (EcoFarm) (20 hds); as well as from semi-intensive managements of small dairy farmers under the supervision of the North Lembang Cooperative Unit (KPSBU Lembang) in two villages of Cilumber (98 hds) and Pasar Kemis (92 hds). Blood samples were also taken via cocsigalis vein of AI (active and non active) HF bulls at the two National AI Stations in Lembang (25 hds) and Singosari (32 hds). Identification of genetic variation was by applying PCR-RFLP method. Three genotypes were identified, namely AA, AB, and BB, resulting two alleles of A and B. Results entirely showed that the frequencies of the BB HF female were very low, with the range of 0%-6% (vs AA genotype= 10%-54% and AB genotype= 46%-85%), despite of the relatively high frequency of the B allele over the A allele (23%-48% vs. 52%-77%). The low frequency of the observed BB females might be due to the limited AI active BB bulls used for services by the two national AI stations (0%-4%).

Polymorphism Identification of Pit1 Gene in Indonesian Buffaloes (Bubalus bubalis) and Holstein-Friesian Cows R Misrianti; C Sumantri; A Farajallah
Media Peternakan Vol. 33 No. 3 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Pit-1 gene has been identified as the pituitary specific transcription factor that regulates the expression of the growth hormone (GH) and prolactin (PRL) genes in the anterior pituitary. The use of polymorphic markers in breeding programmes could make selection more accurate and efficient. A total of 320 Indonesian buffaloes from four populations and 45 FH cows from nine populations were genotyped for polymorphism of Pit1|Hinf1 gene by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) methods. The gene polymorphism was detected only in FH cattle. The frequency of AA, AB, and BB genotype was 2%, 44%, and 53% respectively. The frequency of A allel was 25% and B allel was 75%, but no polymorphism was detected in 320 Indonesia buffaloes.

Association of Polymorphisms Calpastatin Gene with Body Weight of Local Sheep in Jonggol, Indonesia Sutikno Sutikno; M Yamin; C Sumantri
Media Peternakan Vol. 34 No. 1 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Calpastatin (CAST) gene is located on the fifth chromosome of sheep and plays important roles in formation of muscles and meat tenderness after slaughtering. Association of genetic polymorphism in the CAST gene locus MspI and NcoI with body weight was examined in local sheep from Jonggol Animal Science Teaching and Research Unit (JASTRU), Faculty of Animal Science, Bogor Agricultural University. The genotypes for CAST were determined by the PCR-RLFP method. Blood samples were collected from 264 local sheep belonging to JASTRU located in Singosari Village, Bogor District, West Java Province. Extraction of genomic DNA was based on the phenol chloroform method. CAST locus MspI had three genotypes including in MM, MN and NN with frequencies of 0.75, 0.23, and 0.02 respectively. CAST locus NcoI had two genotypes including in MM and MN with frequencies of 0.92, 0.08 respectively. Chi-square test confirmed Hardy-Weinberg equilibrium for the CAST locus MspI and NcoI. There was no significant effects (P>0.05) of CAST locus MspI and NcoI genotypes on body weight of local sheep in JASTRU.

Genetic Polymorphisms of the Coding Region (Exon 6) of Calpastatin in Indonesian Sheep M I.A Dagong; C Sumantri; R R Noor; R Herman; M Yamin
Media Peternakan Vol. 34 No. 3 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Calpastatin (CAST) is an indigenous inhibitor of calpain that involved in regulation of protein turn over and growth. The objective of this research was to identify genetic polymorphisms in the entire exon 6 of calpastatin gene in Indonesian local sheep. A PCR-SSCP method was carried out to identify genetic variation of CAST gene. In total 258 heads of local sheep from 8 populations were investigated, three groups of samples were Thin Tail Sheep (TTS) from Sukabumi, Jonggol, and Kissar. The rest samples were Priangan sheep (PS) from Margawati (Garut meat type) and Wanaraja (Garut fighting type) and Fat Tail Sheep (FTS) from Donggala, Sumbawa, and Rote islands. SSCP analysis revealed that three different SSCP patterns corresponded to three different alleles in the CAST locus (CAST-1, 2, and 3 allele) with five different genotypes. Genetic variation between local sheep populations were calculated based on genotypic and allelic frequencies. Most populations studied were polymorphic, with genotype frequencies of CAST-11, CAST-12, CAST-22, CAST-32, and CAST-33 were 0.286, 0.395, 0.263, 0.046, and 0.007 respectively. CAST-1 and 2 alleles were most commonly found in all populations with total frequency was 0.970, while CAST-3 was a rare allele 0.030 and only found in TTS population. Variation in the CAST gene could be used for the next research as genetic diversity study or to find any association between CAST polymorphism with birth weight, growth trait and carcass quality in Indonesian local sheep.

The Use of Cytochrome b Gene as a Specific Marker of the Rat Meat (Rattus norvegicus) on Meat and Meat Products H Nuraini; A Primasari; E Andreas; C Sumantri
Media Peternakan Vol. 35 No. 1 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Falsification of the origin of livestock meat and its processed with rat meat is a problem that must be overcome to ensure food safety. One way that is often used to detect forgeries by using cytochrome b gene as a marker. The purpose of this study was to create a specific primer derived from cytochrome b sequences in rat (Rattus norvegicus) as the DNA marker to detect any contamination of rat meat on fresh livestock meat and its processed meat products. Meatballs were made from beef meat with the addition of rat 1%-25%, and the meatballs were obtained from traditional markets. DNA extraction was conducted from seven species (goat, chicken, cattle, sheep, pig, horse, and rat) by using phenol-chloroform. The highest success rate in detecting the presence of rat meat in a mixture of beef meatballs at concentration of 15% was 100%. The specific fragment of cytochrome b gene in R. norvegicus has no similarity with the cytochrome b gene from six other species, so it can be used as molecular markers to detect the presence of rat meat contamination in the processed of meat products. Amplified fragment length for goats, chickens, cattle, sheep, pigs, horses, and rats 157, 227, 274, 331, 398, 439 and 603 bp respectively. The amplification of cytochrome b gene in seven species of animals with different fragment length indicated the specificity of cytochrome b gene sequences among species.

A Novel Single Nucleotide Polymorphism in Exon 4 of Insulin-Like Growth Factor-1 Associated with Production Traits in Bali Cattle . Maskur; C Arman; C Sumantri; E Gurnadi; . Muladno
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Insulin-like growth factor-1 (IGF-1) is one of the gene candidates that can be used in selection strategy by using DNA markers (marker assisted selection). Gene candidate strategy is a molecular biology techniques to identify quantitative trait loci directly, with the assumption that genetic variation associated to quantitative trait variation. This study was designed to identify any new mutations in exon 4 that can cause the IGF-1 gene polymorphism and then affect the production traits on Bali cattle. Single nucleotide polymorphism (SNP) discovery was conducted by using the direct sequencing technique. Genetic variation of the genes candidate was identified by using PCR-RFLP technique. The results of this study indicate the presence of a new SNP in exon 4 of IGF-1 gene caused by the T/C transition, which can be identified using Rsa1 restriction enzyme. Genotypic polymorphism of IGF-1/Rsa1 has a significant influence on birth weight, weaning weight and average daily gain of Bali cattle. CC genotype had a birth weight rate, weaning weight and average daily gain of: 15.64±1.83; 83.15±9.00, and 0.439±0.07 respectively, higher than the TT and CT genotype. IGF-1/Rsa1 can be used as a genetic marker for selection of birth weight, weaning weight, and daily body weight gain.

Co-Authors . Irine . Mahmudah . Maskur A Anggraeni A Anggraini A Einstiana A Muzani A O Rini A Primasari A Tapyadi A Winarto ACHMAD FARAJALLAH B Brahmantiyo B Tiesnamurti B W Putra Bambang Ngadji Utomo Bambang Ngaji Utomo C Arman D Perwitasari E Andreas E Gurnadi E M Sari E Sari E.D Gunardi Eko Handiwirawan F Saputra Gurnadi E H Nuraini Han J L I Inounu I Khaerunnisa I Supriatna J F Salamena J.F Salamena Jakaria Jakaria M I.A Dagong M Yamin M Yunus Maria Ulfah Muhammad Ihsan Andi Dagong Nena Hilmia Niken Ulupi Nurhidayat - R Diyono R Herman R Misrianti R Priyanto R R Noor R.R Noor R.R. Noor Ronny R Noor S S Mansjoer Subandriyo . Sutikno Sutikno T Kertanugraha

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