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All Journal Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Journal of Biotechnology and Natural Science
Tristiyanti, Alvia Sefie
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Engineering Immunology & microbiology

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The Effectiveness of Solid Waste Extract from Lemongrass (Cymbopogon citratus) as a Bioinsecticide for Controlling Whitefly (Bemisia tabaci) Pests on Pomelo Plants Tristiyanti, Alvia Sefie; Hayani, Alfitra; Ardianto, Sandi Dimas; Kurniasari, Laeli
Journal of Biotechnology and Natural Science Vol. 3 No. 2 (2023): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i2.9669

Abstract

This research aims to analyze the effect of lemongrass waste extract on the mortality of whitefly and determine the optimal concentration of lemongrass waste extract to eradicate whiteflies within 24 hours. Lemongrass waste extract (leaves and stems) can be obtained through extraction using maceration. The waste is dried and ground to 60 mesh, then dissolved in 96% ethanol and ethyl acetate solvents at a ratio of 1:8 (w/v). Extraction is carried out over variable times of 2, 4, and 6 days. The optimal results are then used to create concentrations by dissolving 250 ml of distilled water in the mother solution, resulting in test solutions of 0, 200, 400, 800, and 1200 mg/L. Observations on the treatments are conducted after 24 hours, and the number of dead whiteflies is counted. Subsequently, the mortality of the whiteflies is analyzed. Based on the research findings, the highest mortality percentage after 24 hours is observed in the ethanol extract, with a dose of 1200 mg/L at 81.81%. After determining the mortality rates, probit analysis is conducted to determine the LC50 (Lethal Concentration 50) value. The optimal concentration for ethanol extract is found to be 1200 mg/L, with an LC50 of 885.102 mg/L. This indicates that lemongrass waste extract is moderately toxic to whiteflies.
Penerapan Metode Microwave-Assisted Extraction (MAE) Berbasis Green Solvent Senyawa Pektin Albedo Jeruk Bali (Citrus Maxima) Amaranti, Ratna Bernika; Indarwati, Dewi; Tristiyanti, Alvia Sefie; Maharani, Farikha; Kurniasari, Laeli
Prosiding Sains Nasional dan Teknologi Vol 11, No 1 (2021): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 11 2021
Publisher : Fakultas Teknik Universitas Wahid Hasyim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36499/psnst.v1i1.5371

Abstract

Buah jeruk bali atau Citrus Maxima merupakan salah satu jenis jeruk yang banyak di budidayakan di Indonesia. Pada umumnya jeruk bali dikonsumsi dalan keadaan segar, sehingga menyebabkan limbah berupa kulit atau albedo dari jeruk bali. Seperti yang diketahui albedo jeruk bali mengandung komponen nutrisi yang kaya akan manfaat, diantaranya senyawa alkaloid, flavonoid, likopen, dan yang paling dominan adalah pektin sebesar 16,68% sampai 21,95%. Pektin sendiri sangat bermanfaat dalam industry pangan maupun farmasi. Sehingga diperlukan ekstraksi pektin albedo jeruk bali menggunakan Microwave Assisted Extraction. Dalam proses ekstraksi umumnya menggunakan pelarut organic. Penggunaan pelarut tersebut memiliki dampak buruk baik kepada operator/peneliti hingga lingkungan. Maka dari itu dilakukan ekstraksi berbasis green solvent jenis baru berupa pelarut eutektik alami (NADES) karena memiliki beberapa keunggulan. Tujuan dari penelitian ini adalah (i) untuk mengetahui rasio terbaik pelarut dalam proses ekstraksi, (ii) mengetahui lama waktu optimum proses ekstraksi, dan (iii) untuk mengetahui daya terbaik pada proses ekstraksi. Hasil ekstraksi terbaik diperoleh pada rasio pelarut 1:5:18 (CAS-H2O), dengan lama waktu ekstraksi optimum pada menit ke 20, dan pada daya 50% dari daya maksimum 800 watt.
Co-Authors Amaranti, Ratna Bernika Ardianto, Sandi Dimas Farikha Maharani Hayani, Alfitra Indarwati, Dewi Laeli Kurniasari