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All Journal Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Acta Aquatica: Aquatic Sciences Journal
Daulay, Anggi Mayulina
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Materials Science & Nanotechnology Other

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Toxicity test of LC-50 (Lethal Concentration) surfactant LAS (Linear Alkylbenzene Sulfonate) against white snapper (Lates calcarifer) fingerlings on a laboratory scale Daulay, Anggi Mayulina; Erniati, Erniati; ˜Akla, Cut Meurah Nurul; Erlangga, Erlangga; Imamshadiqin, Imamshadiqin
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Jurnal Ilmu Perairan, Vol. 9: No. 3 (December, 2022)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v9i3.6780

Abstract

LAS (Linear Alkylbenzene Sulfonate) surfactants are the main cleaning agents from the anionic group found in detergents that are widely used in Indonesia. LAS decomposes in surface water for 1-2 days, in sediments it decomposes for 1-3 days, in marine and estuarine systems it decomposes for 5-10 days. LAS is generally degraded by as much as 90% within 3 months, with a time ranging from 5-30 days (World Health Organization, 1996). LAS surfactants can cause damage to the gill epithelium and blockage of branchiola channels in fish. This study aims to determine the toxic effect of LAS surfactant on the survival of white snapper (Lates calcarifer) fry and to determine the toxicity value of LC50 (Lethal Concentration) of LAS surfactant to white snapper (Lates calcarifer) fry on a laboratory scale. This research was carried out on September 21 - October 04, 2021 at the Marine Science Laboratory, Faculty of Agriculture, Malikussaleh University using laboratory experimental methods with 5 treatments, namely: control treatment A (0 ppm), B (0.83 ppm), C (1.58 ppm). ), D (2.16 ppm), and E (2.91 ppm). The results of this study cause barramundi to experience irregular movements, mouth opening and operculum tend to be fast and irregular, scales are pale and peeling, and cause death, LAS surfactants affect water quality such as pH and dissolved oxygen, but have no effect on salinity and temperature. The 24-hour LC50 value is 1.95 ppm, the 48-hour LC50 value is 1.86 ppm, the 72-hour LC50 value is 1.70 ppm, and the 96-hour LC50 value is 1.58 ppm.Key words: LAS surfactant; LC50; toxicity; white snapper
Pendekatan Metabarcoding eDNA untuk Mendeteksi Keanekaragaman Spons Laut: Sistematik Literatur Review dan Bibliometrik Analisis Daulay, Anggi Mayulina; Subhan, Beginer; Prasetyo, Andhika Prima; Bengen, Dietriech Geoffrey
Jurnal Kelautan Vol 18, No 3: Desember (2025)
Publisher : Department of Marine Sciences, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/jk.v18i3.32404

Abstract

ABSTRAKPenelitian ini bertujuan menganalisis perkembangan, fokus tematik, dan arah riset terkait penggunaan environmental DNA (eDNA) metabarcoding untuk mendeteksi dan mengidentifikasi spons laut (Porifera). Pendekatan yang digunakan adalah systematic literature review (SLR) berbasis PRISMA dan analisis bibliometrik terhadap artikel terindeks Scopus tahun 2009–2025. Dari 116 publikasi awal, 42 artikel memenuhi kriteria inklusi dan dianalisis lebih lanjut menggunakan VOSviewer untuk mengidentifikasi tren publikasi, pola kolaborasi penulis-negara, serta struktur klaster kata kunci. Hasil menunjukkan peningkatan signifikan publikasi sejak 2017, dengan kontribusi terbesar berasal dari Britania Raya, Spanyol, dan Amerika Serikat. Analisis kata kunci menghasilkan lima klaster utama yang mencerminkan arah riset global, yaitu (1) genetika dan biodiversitas, (2) mikrobioma dan metagenomik, (3) ekologi terumbu karang, (4) invertebrata laut, serta (5) lingkungan ekstrem. Meskipun eDNA metabarcoding terbukti sensitif untuk mendeteksi komunitas spons, tantangan utama yang ditemukan mencakup keterbatasan marker gen yang belum universal serta kelengkapan basis data taksonomi yang masih rendah. Novelty penelitian ini terletak pada penggabungan SLR dan bibliometrik secara khusus pada spons laut, sehingga mampu mengungkap kesenjangan geografis, keterbatasan metodologis, serta menawarkan rekomendasi strategis berupa standardisasi protokol, penguatan basis data referensi lokal, dan perluasan kolaborasi internasional, terutama di wilayah megabiodiversitas seperti Indonesia.Kata Kunci: eDNA, metabarcoding, Porifera, bibliometric, keanekaragaman hayati.ABSTRACTThis study analyzes the development, thematic focus, and research direction of environmental DNA (eDNA) metabarcoding applied to the detection and identification of marine sponges (Porifera). A systematic literature review (SLR) following PRISMA and a bibliometric analysis of Scopus-indexed publications from 2009–2025 were conducted. From an initial 116 records, 42 articles met the inclusion criteria and were analyzed using VOSviewer to identify publication trends, author–country collaborations, and keyword cluster structures. Results show a substantial increase in publications since 2017, with major contributions from the United Kingdom, Spain, and the United States. Keyword analysis revealed five major thematic clusters: (1) genetics and biodiversity, (2) microbiome and metagenomics, (3) coral reef ecology, (4) marine invertebrates, and (5) extreme environments. Although eDNA metabarcoding demonstrates high sensitivity for sponge detection, key challenges include non-universal genetic markers and incomplete taxonomic reference databases. The novelty of this study lies in integrating SLR and bibliometric approaches specifically for marine sponges, allowing the identification of geographic gaps, methodological limitations, and providing strategic recommendations for protocol standardization, the development of local reference databases, and enhanced international collaboration, particularly in megabiodiverse regions such as Indonesia.Keywords: eDNA, metabarcoding, Porifera, bibliometric analysis, biodiversity.
Co-Authors Andhika Prima Prasetyo Beginer Subhan Dietriech Geoffrey Bengen Erlangga Erlangga Erniati Erniati Imamshadiqin, Imamshadiqin ˜Akla, Cut Meurah Nurul