Article Per Year (5 Year)
p-Index From 2020 - 2025
0.61
P-Index
This Author published in this journals
All Journal Jurnal Ilmiah Farmasi Pharmaciana: Jurnal Kefarmasian Jurnal Farmasi Sains dan Komunitas
Safira, Rizka
Unknown Affiliation
Medicine & Pharmacology

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Model Hewan Uji Agen Terapeutik Alami pada Stroke Iskemik Botutihe, Lisa Agustina; Safira, Rizka; Kintoko
Jurnal Ilmiah Farmasi Vol. 21 No. 2 (2025): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol21.iss2.art2

Abstract

Background: Ischemic stroke is an acute neurological injury resulting from focal damage to the central nervous system due to vascular obstruction and subsequent decrease in cerebral blood flow. Numerous animal models of ischemic stroke have been established to investigate its mechanism, pathophysiology, and risk factors. The animal model of ischemic stroke includes a global ischemia model and a focal ischemia model. This article describes various parameters, including hematological, biochemical, cytological, histological, and molecular factors, along with diverse biomarkers, that may support research in the development of novel, safer, and more effective therapeutic agents for ischemic stroke using animal models.Objective: This research seeks to determine the appropriate test animal model and parameters for ischemic stroke experiments.Method: A complete literature search was conducted across multiple databases, including NCBI, PubMed, and additional sources.Results: According to reference studies, the animal model test in the ischemic stroke experiment comprised a focal ischemia model and a global ischemic model. The focal ischemic model is more pertinent to ischemic stroke in humans compared to the global ischemic model. In addition, focal ischemic models, including Middle Cerebral Artery Occlusion (MCAO), have been utilized in over 40% of 2,582 nerve protection trials. The wide variety of test animal models possesses distinct advantages and disadvantages, making it crucial to select the appropriate model. The parameters of ischemic stroke, including hematology, biochemistry, cytology, histology, and molecular analysis, together with their biomarkers, can help in identifying the incidence of ischemic stroke in test animals.Conclusion: The focused ischemia model is a more pertinent animal model for ischemic stroke in relation to humans than the global ischemic model. Parameters utilized for the identification of ischemic stroke encompass hematology, biochemistry, cytology, histology, and molecular biology.
Combination of Spirulina platensis powder and Stichopus variegatus powder against Bcl2 expression in the hippocampus of dementia Rats Botutihe, Lisa Agustina; Safira, Rizka; Yuliani, Sapto; Kintoko, Kintoko
Pharmaciana Vol. 14 No. 1 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i1.26205

Abstract

Spirulina (Spirulina platensis) and golden sea cucumber (Stichopus variegatus) are known to have antioxidant activity that has the potential to prevent neurodegeneration disease. The aim of this study was to examine the effect of the combination of spirulina and golden sea cucumber on Bcl2 gene expression in pyramidal hippocampus cells of trimethyltin-induced dementia (TMT) rats. The study used Sprague Dawley rats  which were divided into 6 groups, namely the normal control group (CMC-Na and NaCl 0.9%), pain control (CMC-Na and TMT), positive control (citicoline dose 200 mg / kg and TMT) and test control injected with TMT and given a combination of spirulina and golden sea cucumber dose 200 mg/KgBB with three ratios namely 3: 1, 1: 1 and 1: 3. Extract and citicoline were given on day 1 to day 28, while TMT injection was given a single dose of 8 mg/KgBB on day 8. On the 36th day the rats were sacrificed, brains were removed and the right hemispherium cerebri was fed to 10% formalin in pbs. After 6 days the hippocampus was separated for immunohistochemical observation. The test result data was statistically analyzed with a one-way ANOVA test then followed by post hoc tukey to see the differences between groups. Results showed the combination of spirulina and golden sea cucumber can increase the expression of the Bcl2 gene in the hippocampus. The combination of spirulina and golden sea cucumber (ST1: 3) dose of 200 mg / kg body weight was able to increase hippocampus Bcl2 expression with the number of Bcl2 cell expression almost the same as citicoline in both CA1 and CA2-CA3 regions.
Effectiveness of the Combination of Spirulina platensis and Stichopus variegatus on Prevention of Caspase-3 Gene Expression of Dementia Rat Models Safira, Rizka; Botutihe, Lisa Agustina; Yuliani, Sapto; Kintoko, Kintoko
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 21, No 1 (2024)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/jpsc.006051

Abstract

The death of cells in the brain, especially the hippocampus, manifests in a decrease in memory, language, and behavior. Golden sea cucumber (Stichopus variegatus) andblue-green algae (Spirulina platensis) are reported to have high antioxidant content which has the potential to prevent cell death in the brain due to oxidative stress thereby preventing memory decline.This study aimed to investigate the effect of the combination of Stichopus variegatus and Spirulina platensis on preventing caspase 3 gene expression in pyramidal hippocampal cells in a rat model of trimethlytin-induced dementia (TMT). This study used Sprague Dawley rats, about 2 months old, weighing 180-200 g, divided into 6 treatment groups, with each group consisting of 8 rats. The hippocampus was taken from the right cerebral hemisphere for histological observations of pyramidal caspase-3 gene expression in the CA1 and CA2-CA3 regions.The combination of Spirulina platensis and Stichopus variegatus with a dose 200 mg/kg BB can prevent the expression of caspase-3 in the CA1 and CA2-CA3 areas of the hippocampus. Conclusion: The combination of spirulina and golden sea cucumber extracts has the potential to prevent caspase 3 gene expression in pyramidal cells of TMT-induced dementia rat models. 
Co-Authors Botutihe, Lisa Agustina Kintoko Kintoko Kintoko Sapto Yuliani