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All Journal Plumula : Berkala Ilmiah Agroteknologi International Journal on Food, Agriculture and Natural Resources (IJFANRES)
Indrawan, Arga Dwi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Energy Immunology & microbiology Materials Science & Nanotechnology

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The Potency of Streptomyces spp. from Shallot Land as Entomopathogen of Onion Caterpillar Pest Spodoptera exigua Pratista, Ariq Dies; Windriyanti, Wiwin; Suryaminarsih, Penta; Mujoko, Tri; Indrawan, Arga Dwi; Harsiyanti, Harsiyanti
International Journal on Food, Agriculture and Natural Resources Vol 6, No 4 (2025): IJ-FANRES
Publisher : Food, Agriculture and Natural Resources - NETWORKS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46676/ij-fanres.v6i4.569

Abstract

Spodoptera exigua is a major pest of shallots (Allium ascalonicum L.), capable of causing up to 100% yield loss if uncontrolled. Streptomyces sp. is a soil-dwelling Actinomycetes bacterium that can be used to control S. exigua. This study aimed to determine the potential of Streptomyces spp. isolates from shallot fields as an entomopathogen against S. exigua. The research employed a Factorial Complete Randomized Design (CRD). The first factor consisted of one Streptomyces spp. isolate obtained from the Pare-Kediri region (BMP) and three isolates from Sidera-Palu (BMS1, BMS2, and BMS3). The second factor was the concentration of the Streptomyces sp. suspension consist of 0%; 5%; 10%; and 15% with three replication per treatment (48 units total). Data on larval mortality and damage intensity were analyzed using RStudio via ANOVA and DMRT (α=5%). The BMP (15%) treatment demonstrated the highest efficacy, achieving 76.67% larval mortality while maintaining the lowest damage intensity of 0.20% on shallot plants. These results highlight BMP isolate at 15% concentration as the most promising biocontrol agent and support its further field evaluation as a sustainable alternative to chemical insecticides.
Potensi Trichoderma spp. dari Koleksi Tanah Tanaman Perkebunan asal Jawa Timur sebagai Antagonis Fusarium oxysporum Amara, Khansa; Indrawan, Arga Dwi; Af’idzatuttama
Plumula : Berkala Ilmiah Agroteknologi Vol. 13 No. 2: Juli 2025
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/plumula.v13i2.261

Abstract

Aplikasi Trichoderma spp. hasil eksplorasi pada tanah tanaman sehat dapat berpotensi dalam pengendalian penyakit layu yang ramah lingkungan dan dapat diterapkan dalam jangka panjang. Penelitian ini bertujuan untuk menilai kualitas spora dan kemampuan antagonis Trichoderma spp. yang diisolasi dari tanah tanaman kakao, kopi, dan cengkeh terhadap F. oxysporum secara in vitro. Penelitian disusun dengan rancangan acak lengkap (RAL), dengan perlakuan jenis Trichoderma dari sumber sampel tanah berbeda. Variabel pengamatan meliputi: kerapatan dan viabilitas spora, serta tingkat hambatan relatif yang selanjutnya dianalisis ragam (ANOVA) menggunakan SAS on Demand, dan uji lanjut Tukey pada taraf 5%. Seluruh isolat Trichoderma spp. menunjukkan hasil melebihi kriteria penilaian standar mutu sebagai agens hayati berdasarkan SNI 8027.3:2014, dengan kerapatan spora 108 spora/ml. Namun, hanya isolat Trichoderma spp. asal tanah tanaman kopi yang memiliki kualitas spora dan persentase penghambatan terbaik dalam menekan pertumbuhan diameter koloni F. oxysporum, yaitu sebesar 59%.
Co-Authors Af’idzatuttama Amara, Khansa Harsiyanti, Harsiyanti Penta SURYAMINARSIH Pratista, Ariq Dies Tri MUJOKO Windriyanti, Wiwin