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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences JSFK (Jurnal Sains Farmasi & Klinis) Berita Biologi
Janatiningrum, Isra
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Isolation of Escherichia coli bacteriophages from Domestic Wastewater in Tangerang Selatan Amelia, Puteri; Adila, Ersa Fiki; Novitri, Suci Ahda; Janatiningrum, Isra
JSFK (Jurnal Sains Farmasi & Klinis) Vol 10 No 3 (2023): J Sains Farm Klin 10(3), Desember 2023
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.10.3.368-373.2023

Abstract

In recent years, there has been a continuous increase in the prevalence of antibiotic resistance among bacteria responsible for infectious diseases. Several studies have shown that the occurrence of the COVID-19 pandemic is a major factor contributing to this rapid increase. To overcome this challenge, one promising approach is the use of bacteriophages as a treatment option. Therefore, this study aims to isolate E. coli lytic bacteriophages as an alternative antibiotic for treating secondary bacterial infections of COVID-19. Isolation of bacteriophages was carried out using the plaque assay method with double layer agar technique. The isolate obtained was purified and tested for effectiveness in lysing E. coli ATCC 25922 bacterial cells. The results showed that E. coli had resistance to antibiotics, such as Amoxicillin, Clindamycin, Ciprofloxacin, Azithromycin, and Trimethoprim/sulfamethoxazole. BEB1 isolate obtained from domestic waste had activity in inhibiting the growth of the test bacteria with an effectiveness of 72% at the 25th hour. Based on these findings, it had the potential to be developed as an alternative antibiotic agent to overcome infectious diseases caused by E. coli ATCC 25922.
Antibacterial Activity Against Acne-Causing Bacteria and Compound Profiling of Bacteria Associated with the Black Gum Sea Cucumber (Holothuria leucospilota) Arumawati, Novia Ashilia; Janatiningrum, Isra; Fitriyanti, Fitriyanti; Anggia, Vivi
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 31, No 1 (2026): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.31.1.79-86

Abstract

Sea cucumbers (Holothuria leucospilota) have significant potential as sources of antibacterial compounds. These organisms live in association with bacteria that produce secondary metabolites exhibiting biological activities similar to those of their hosts, including antibacterial effects. Acne is an inflammatory condition that can be exacerbated by acne-causing bacteria such as Staphylococcus epidermidis and Propionibacterium acnes. Although antibiotics are commonly used for treatment, their effectiveness has increasingly been compromised by the emergence of antibiotic resistance. Bacteria associated with H. leucospilota therefore represent a promising alternative source of antibacterial agents due to their ability to produce bioactive secondary metabolites. This study aimed to characterize the macroscopic and microscopic diversity of these bacteria, evaluate their antibacterial activity against S. epidermidis and P. acnes, and analyze the chemical profiles of their ethyl acetate fractions. Bacterial isolation was conducted using Sea Water Complete Agar (SWCA), and antibacterial activity was assessed using the TLC bioautography method with iodonitrotetrazolium chloride (INT) as a growth indicator. A total of 12 bacterial isolates were obtained, comprising 10 isolates from the internal tissues and 2 isolates from the external surface of H. leucospilota. Antibacterial screening showed that 11 of the 12 isolates were active against S. epidermidis, while 8 isolates exhibited activity against P. acnes. Phytochemical screening indicated that phenolic and terpenoid compounds were the bioactive constituents responsible for the observed antibacterial activity.
KERAGAMAN DAN POTENSI BAKTERI ENDOFIT MATOA (Pometia pinnata) SEBAGAI PENGHASIL AGEN ANTIMIKROBA Janatiningrum, Isra; Najah, Lailatun; Permatasari, Intan; Fitriyanti; Anggia, Vivi; Putri, Eka
Berita Biologi Vol 25 No 1 (2026): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2026.7177

Abstract

Bakteri endofit merupakan mikroorganisme yang hidup pada jaringan tanaman tanpa menimbulkan dampak negatif, namun memberikan manfaat bagi inangnya. Matoa (Pometia pinnata) diketahui memiliki senyawa antimikroba. Senyawa metabolit sekunder yang ada pada tanaman tidak terlepas dari interaksinya dengan mikroba endofit yang hidup di jaringan tanaman. Meningkatnya resistensi antibiotik menyebabkan pencarian senyawa antibiotik terus dilakukan. Bakteri endofit dari P. pinnata diharapkan dapat menjadi sumber senyawa antibiotik baru. Penelitian ini bertujuan untuk mengisolasi, mengetahui keanekaragaman bakteri endofit tanaman matoa yang dapat dikulturkan, dan mengetahui potensinya sebagai antimikroba. Metode skrining untuk mengetahui aktivitas antimikroba menggunakan metode agar plug dan metode difusi cakram. Hasil isolasi bakteri endofit dari tanaman matoa diperoleh 32 isolat bakteri. Karakterisasi morfologi dari 32 isolat bakteri endofit yang diperoleh menunjukkan bahwa warna koloni terbanyak adalah signal white, traffic yellow dan light pink. Uji skrining aktivitas antimikroba diperoleh 2 isolat yang mempunyai daya hambat terhadap seluruh mikroba uji yaitu DMS4 dan PK1. Ekstrak isolat DMS4 dan PK1 diuji antimikroba, hasilnya menunjukkan adanya penghambatan pada konsentrasi 1000 ppm pada ekstrak etil asetat. Berdasarkan analisis sekuensing gen 16S rRNA isolat PK1 mempunyai kemiripan 99,18% dengan Stenotrophomonas rhizophila dan DMS4 99,85% mirip dengan Brucella haematophila.  
Co-Authors Adila, Ersa Fiki Amelia, Puteri Arumawati, Novia Ashilia Eka Putri Fitriyanti Fitriyanti Fitriyanti Intan Permatasari Najah, Lailatun Novitri, Suci Ahda Vivi Anggia, Vivi