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Campylobacter jejuni seroepidemiology in native chicken Rosyidi, Anwar; Budiharta, S; Asmara, W; Yudhabuntara, D
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 3 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.581 KB) | DOI: 10.14334/jitv.v17i3.706

Abstract

Campylobacter jejuni is responsible for about 90% of cases of Campylobacteriosis in humans with gastroenteritis. Healthy chickens can carry Campylobacter spp. in the intestinal tract. Efforts to reduce exposure to Campylobacteriosis by humans may be enhanced by knowledge of its prevalence in poultry. This study aimed to identify factors associated with seropositive response to C. jejuni in native chickens in Mataram. Detection of C. jejuni was accomplished using an immunochromatographic serological method. Association between Campylobacter jejuni seropositive response as the dependent variable with various independent variables was analyzed using χ² (Chi square) and Odds Ratio (OR). A total of 216 chicken samples were examined and 44 chicken owners were interviewed and their farms examined. Results showed the prevalence of serological response to C. jejuni in chicken samples to be as high as 35.6% and that as many as 70.5% of farms had affected chickens.  Age of the chicken was the variable most closely associated with incidence of seropositive response, birds older than 3 months more likely to be affected. Variables at the farm level associated with variation in seropositive response were cage type, cage floor material, and origin of drinking water, surface water sources being less desirable. Key Words: Campylobacter jejuni, Seroepidemiology, Native Chicken, Mataram
Phenotypic and Genotypic Detection of Campylobacter jejuni at Local Chicken and Chicken Meat Rosyidi, A; Budhiharta, S; Asmara, W; Yudhabuntara, D
ANIMAL PRODUCTION Vol 12, No 2 (2010): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The Objective of this study was to identify the existence of Campylobacter jejuni based on  phenotypic and genotypic characteristic in local chicken and chicken meats. Samples of local chicken  intestine and meat were tested  for the bacterial existence. Phenotypic examination was carried out by means of cultivation followed  by gram staining and biochemical tests. Genotypic examination was conducted by polymerase chain reaction (PCR) using genus specific16S rRNA gene at 816 bp  and membrane-associated protein A (mapA) gene  at 589 bp as Campylobacter jejuni species-specific gene. The result of phenotypic detection revealed the  existence of  Campylobacter spp as  gram negative, curved rod shape, oxidase positive,  urease negative and motile. Genotypic examination also indicated the existence of  bacteria using both primers. However, no Campylobacter jejuni detected from meat of the chickens. The results suggest that the method of PCR using a primer  detecting species-specific gene of Campylobacter jejuni gives a rapid and accurate detection of the bacteria as compared to that using phenotypic and biochemical test. Identification of Campylobacter spp from chicken meats  should be improved with enrichment method and sample collection. (Animal Production 12(2): 128-134 (2010)Key Words: Campylobacter jejuni, mapA gene, local chicken
Virgin Coconut Oil Increases the Productivity of Broiler Chicken Post Avian Influenza Vaccination Yuniwarti, EYW; Asmara, W; Artama, WT; Tabbu, CR
ANIMAL PRODUCTION Vol 14, No 3 (2012): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract. Chicken productivity is not only determined by body weight increase and feed efficiency but also disease resistance. Avian influenza (AI) is still an endemic in Indonesia. Highly mutative characteristic of AI causes unsuccessful vaccination to preventing chicken mortality; therefore, feed modulation alternatives are sought to raise body weight and body immune as well. Virgin coconut oil (VCO) contains fatty acid potential as antimicrobe and antivirus; VCO intake is therefore expected to increase chicken body immune. This research aimed at feed modulation to increase broiler chicken productivity. Forty broiler chicken of one day old (DOC) were used and the research applied Completely Randomized Factorial Design in which factor one was two vaccine levels namely AI-vaccinated chickens and AI-unvaccinated chickens. Factor two used four levels of VCO: 0, 5, 10, 15 mL/kg feed. DOC chickens were divided into eight treatment groups and repeated in five experiment units. Feed and water were given ad libitum. The result demonstrated that in spite of heterophile increase in AI-vaccinated VCO-given chickens, heterophile/lymphocyte ratio and feed intake were not significantly different among all treatment groups. With the highest body weight found in AI-vaccinated chickens given 10ml/kg feed VCO, it could therefore be concluded that VCO intake of 10mL/kg feed could raise body weight.Key words: heterophile, heterophile/lymphocyte ratio, feed intake, body weightAbstrak. Produktivitas ayam tidak hanya ditentukan oleh kenaikan bobot badan dan efisiensi pakan, tetapi juga ketahanan terhadap penyakit. Avian influenza (AI) masih merupakan wabah endemis di Indonesia. Sifat AI yang mudah bermutasi menyebabkan vaksinasi tidak selalu berhasil untuk mencegah kematian ayam, sehingga dicari alternatif modulasi pakan untuk meningkatkan bobot badan dan kekebalan tubuh. Virgin coconut oil (VCO) mengandung potensi asam lemak sebagai antimikroba dan antivirus, sehingga asupan VCO diharapkan dapat meningkatkan kekebalan tubuh ayam. Penelitian ini bertujuan untuk modulasi pakan untuk meningkatkan produktivitas ayam broiler. Empat puluh ayam broiler umur satu hari  (DOC) digunakan dalam penelitian yang menerapkan Rancangan Acak Lengkap Faktorial dengan faktor pertama dua level vaksinasi  yaitu ayam divaksin AI dan tidak divaksin AI. Faktor kedua adalah empat level VCO: 0, 5, 10, 15 mL  kg pakan. DOC ayam dibagi menjadi delapan kelompok perlakuan dan diulang dalam lima unit percobaan. Pakan dan air minum diberikan ad libitum. Hasilnya menunjukkan bahwa meskipun adanya kenaikan heterofil pada ayam yang divaksin AI dan diberi VCO, rasio heterofil/limfosit dan konsumsi pakan tidak berbeda secara signifikan pada semua kelompok perlakuan. Dengan bobot badan tertinggi ditemukan pada ayam yang divaksin AI dan diberi VCO 10 ml/kg pakan, maka dapat disimpulkan bahwa asupan VCO 10 mL/kg pakan dapat meningkatkan bobot badan. Kata kunci: heterophil, rasio heterophil/limfosit, konsumsi pakan, bobot badan EYW Yuniwartiet al/Animal Production 14(3):192-198, September 2012
Campylobacter jejuni seroepidemiology in native chicken Anwar Rosyidi; S Budiharta; W Asmara; D Yudhabuntara
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 3 (2012): SEPTEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.581 KB) | DOI: 10.14334/jitv.v17i3.706

Abstract

Campylobacter jejuni is responsible for about 90% of cases of Campylobacteriosis in humans with gastroenteritis. Healthy chickens can carry Campylobacter spp. in the intestinal tract. Efforts to reduce exposure to Campylobacteriosis by humans may be enhanced by knowledge of its prevalence in poultry. This study aimed to identify factors associated with seropositive response to C. jejuni in native chickens in Mataram. Detection of C. jejuni was accomplished using an immunochromatographic serological method. Association between Campylobacter jejuni seropositive response as the dependent variable with various independent variables was analyzed using χ² (Chi square) and Odds Ratio (OR). A total of 216 chicken samples were examined and 44 chicken owners were interviewed and their farms examined. Results showed the prevalence of serological response to C. jejuni in chicken samples to be as high as 35.6% and that as many as 70.5% of farms had affected chickens.  Age of the chicken was the variable most closely associated with incidence of seropositive response, birds older than 3 months more likely to be affected. Variables at the farm level associated with variation in seropositive response were cage type, cage floor material, and origin of drinking water, surface water sources being less desirable. Key Words: Campylobacter jejuni, Seroepidemiology, Native Chicken, Mataram