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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Animal Production : Indonesian Journal of Animal Production
A Rosyidi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Phenotypic and Genotypic Detection of Campylobacter jejuni at Local Chicken and Chicken Meat Rosyidi, A; Budhiharta, S; Asmara, W; Yudhabuntara, D
ANIMAL PRODUCTION Vol 12, No 2 (2010): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The Objective of this study was to identify the existence of Campylobacter jejuni based on  phenotypic and genotypic characteristic in local chicken and chicken meats. Samples of local chicken  intestine and meat were tested  for the bacterial existence. Phenotypic examination was carried out by means of cultivation followed  by gram staining and biochemical tests. Genotypic examination was conducted by polymerase chain reaction (PCR) using genus specific16S rRNA gene at 816 bp  and membrane-associated protein A (mapA) gene  at 589 bp as Campylobacter jejuni species-specific gene. The result of phenotypic detection revealed the  existence of  Campylobacter spp as  gram negative, curved rod shape, oxidase positive,  urease negative and motile. Genotypic examination also indicated the existence of  bacteria using both primers. However, no Campylobacter jejuni detected from meat of the chickens. The results suggest that the method of PCR using a primer  detecting species-specific gene of Campylobacter jejuni gives a rapid and accurate detection of the bacteria as compared to that using phenotypic and biochemical test. Identification of Campylobacter spp from chicken meats  should be improved with enrichment method and sample collection. (Animal Production 12(2): 128-134 (2010)Key Words: Campylobacter jejuni, mapA gene, local chicken
Penghambatan Proliferasi Limfosit Mencit Balb/c oleh Ekstrak Testis Sapi Bali: Peran TGF-β S N Depamede; A Rosyidi
Media Peternakan Vol. 32 No. 2 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Testis extract (ET) of several animals such as rats, mice, boars, and bulls, has been reported able to suppress lymphocyte proliferation. In order to elucidate if ET of bali cattle has the suppressive activity, the present study was conducted. ET was isolated aseptically from the testis of bali cattle slaughtered at a slaughter house in Mataram, Lombok. After decapsulating, the testis were minced, homogenized (1 g tissue per 3 ml phosphate buffered saline, PBS, pH 7.2). The ET (40, 20, and 10µg/ml) was added on to lymphocytes isolated from Balb/c spleen, then cultured for 72 hours in RPMI media containing 10% foetal bovine serum in 96 well plates with the concentration of 250,000 cells per well, with- or without concanavalin A (10 µg/ml), or IL-2 (0.2 ng/ml). After 72 h of culture, cell proliferation was examined by colorimetric assay. The results showed that bali cattle ET were able to suppress the proliferation of lymphocytes in vitro through the process of apoptosis. Western blot analysis revealed that bali cattle ET contain TGF-β1. The suppression effects of the ET were eliminated by anti-TGF-β1 antibody. It can be concluded that ET of bali cattle expressed TGF-β  which inhibited lymphocyte proliferation through an apoptotic cell mechanism. The actual roles of TGF-β  in the bali cattle testis need to be elucidated further. Key words: bali cattle testis, lymphocyte, proliferation, TGF-β1, apoptosis
Co-Authors D Yudhabuntara S Budhiharta, S Sulaiman Ngongu Depamede W Asmara