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All Journal Jurnal Primatologi Indonesia Jurnal Belantara Journal of Tropical Biodiversity
Sri Suci Utami Atmoko
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Education Environmental Science Immunology & microbiology Medicine & Pharmacology

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Teknik Analisis Non-Invasif Mitokondria DNA (MtDNA) BILOU (Hylobates klossii, Miller 1903) Melalui Polymerase Chain Reaction Ike N. Nayasilana; Sri Suci Utami Atmoko; Firman .
Jurnal Primatologi Indonesia Vol. 7 No. 1 (2010)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7643.287 KB)

Abstract

Abstrak : Teknik analisis non-invasif untuk mitochondria DNA bilou (Hylobates klossii) dengan Polymerase Chain Reaction (PCR) adalah teknik molekuler yang dapat mendukung kesejahteraan hewan. Analisis non-invasif dari sampel tinja dapat menentukan sampel DNA dari daerah kontrol pada rRNA 12S. Sampel tinja dikumpulkan dari tiga pulau yang berbeda (Sipora, Siberut dan Pagai) dengan total 39 sampel yang diekstraksi untuk DNA. Analisis dilakukan di laboratorium Genetika Zoologi LIPI, Cibinong pada bulan Juli sampai Agustus 2005. Hasil tes PCR yang digunakan Qiagen QIAamp ® DNA stool mini kit dan elektroforesis dapat menunjukkan bahwa sampel DNA dapat ditampilkan bahkan dalam konsentrasi terendah dari pengukuran spektrofotometer. Dengan demikian, hal itu dapat menjadi referensi baru di penelitian masa depan sebagai indikator konservasi manajemen yang sesuai dengan peraturan kesejahteraan hewan yang baik. Polaroid foto dari rRNA 12S (L = 1.091, H = 1478) yang diambil dengan transluminator UV dan kamera Polaroid MP4 dari produk PCR dengan annealing digunakan pada suhu 50oC dalam 30 detik, menunjukkan hasil dari pita penanda elektroforesis DNA berukuran antara 750 sampai 815 pb DNA ladder.Abstract: Polymerase Chain Reaction is a non invasive molecular technique that can support animal welfare.  Non-invasie analysis from fecal sample can determine DNA samples from the control region on 12S rRNA.  Fecal samples were collected from three different islands (Sipora, Siberut and Pagai) with a total of 39 samples were extracted for DNA.  The analyses were done at the laboratory of Genetic Zoology LIPI, Cibinong in July until August 2005. The results of the PCR test which used Qiagen QIAamp® DNA stool mini kit and electrophoresis can show that the sample displays the quality DNA from the fecal sample even in the lowest concentration of the spectrophotometer measurements.  Thus, it has become a new reference in future research as an indicator of good management conservation in line with excellent animal welfare rules. The polaroid photos of the 12S rRNA (L=1091, H=1478) taken with UV transluminator and MP4 polaroid camera from the PCR products with annealing used at 50oC in 30 seconds, shows the results of this running process of electrophoresis DNA sized marker band processes between 750 until 815 pb DNA ladder.Key words: Hylobates klossii, bilou, mtDNA, PCR, 12S rRNA, non-invasive technique.
NESTING BEHAVIORAL ADAPTATIONS OF ORANGUTAN (PONGO PYGMAEUS MORIO) IN COAL MINING AREA IN EAST KALIMANTAN Liza Niningsih; Sri Suci Utami Atmoko; Hadi S Alikodra; Yeni A Mulyani
Jurnal Belantara Vol 4 No 2 (2021)
Publisher : Forestry Study Program University Of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1255.701 KB) | DOI: 10.29303/jbl.v4i2.811

Abstract

Coal mining changed forest structure and composition, it caused large negative impacts on the orangutan. In order to mitigate those negative effects, it is crucial to understand their nesting behavior adaptation in mining area. We investigated 74 times nested event in coal mining rehabilition area (CMRA) in East Kutai and 123 times in the Kutai National Park (Prevab) from October 2013 to September 2014 and include only night nests. We compared both habitats. Orangutans in CMRA built the nest later than in Prevab and used 15 species of trees as nests sites intensively on Senna siamea and Gmelina arborea. In Prevab, 35 species with the higher frequencies on Eusideroxylon zwageri and Dracontomelon dao. The average diameter of nest trees in CMRA smaller than Prevab. The height of nest trees in CMRA with the highest frequency was in 10.1-15 m, while in Prevab was as in 20.1-25 m. Orangutans in CMRA nested at the height of <15 m, lower than in Prevab was >20 m. Reused nest in CMRA was higher than in Prevab. Orangutans in CMRA more often built nests at the peak and limb, while in Prevab at the limb and peak. Orangutans in CMRA had learned to utilize various species and dimensions of trees as the place to build nests.
Distribution and Abundance of Javan Primates in Gunung Sanggabuana, Karawang, West Java Lindayu, Feriska; Utami, Marcelita Putri; Saidatinah, Salsa Mumtaz; Al Hanif, Syivaul Jannah; Priandani , Tasya Azzahra; Atmoko, Sri Suci Utami
Journal of Tropical Biodiversity Vol 5 No 1 (2024): December 2024
Publisher : Universitas Nasional Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59689/bio.v5i1.247

Abstract

Indonesia, as the country with the highest number of primate species in Asia, also holds the largest number of endemic primates, including five species on the island of Java. This study aimed to assess the distribution and abundance of primate species in Gunung Sanggabuana, Karawang, West Java, one of the closest tropical rainforests to Jakarta. Field surveys were conducted during the dry season (11–14 September 2023) by the Primate Student Team of Field Biology Study (SBL). Recce transects were used for primate censuses and fruit trails for recording fruit-bearing plant abundances along two paths: the tourist area and semi-tourist area. Results indicated three primate species were directly encountered: Javan langur (Trachypithecus mauritius), Surili (Presbytis comata), and Javan gibbon (Hylobates moloch). In the Kejayaan-Burahol trail, Javan langurs were observed at 1 group/km (1–5 individuals), Surili at 0.5 groups/km (4 individuals), and Javan gibbons at 1 group/km (4–6 individuals). The Cigentis-Cipiit trail yielded higher Javan langur abundance at 1.5 groups/km (5–8 individuals). The dominance of Javan langur was noted with a low value of 0.160. These findings provide insight into primate distribution and abundance, contributing to conservation efforts in Gunung Sanggabuana and other Javan rainforests.
Changes in the Home Range of Bornean Orangutans (Pongo pygmaeus wurmbii) Before and After Forest Fires at Tuanan Research Station, Central Kalimantan Hasan, Silvia; Sri Suci Utami Atmoko
Journal of Tropical Biodiversity Vol 6 No 1 (2025): December 2025
Publisher : Universitas Nasional Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59689/bio.v6i1.324

Abstract

The Bornean orangutan (Pongo pygmaeus wurmbii), an endangered species native to the tropical forests of Borneo, plays a crucial role in the ecosystem as a keystone species and seed disperser. However, habitat degradation, particularly from forest fires, poses a significant threat to their survival. This study investigates the impact of forest fires on the home ranges of Bornean orangutans at the Tuanan Orangutan Research Station (TORS), Central Kalimantan, Indonesia. Data were collected before and after the 2019 forest fire, focusing on changes in orangutan movement patterns and their responses to altered fruit availability. The results showed that, following the fire, most observed individuals decreased their home range size, except for the flanged male (Dado) and the adult female (Juni), who expanded their home ranges. The forest fire led to a significant reduction in fruit tree productivity, which was reflected in the fluctuating fruit availability index (FAI). Additionally, smoke from the fire disrupted pollination, further impacting fruit production and orangutan foraging behavior. Kernel Density Estimator (KDE) analysis revealed changes in the movement paths and home-range sizes of the orangutans before and after the fire. These findings highlight the adaptability of orangutans to habitat changes induced by fire, and the importance of understanding how fire-related environmental changes affect orangutan survival and behavior. The study underscores the need for practical conservation efforts to mitigate the effects of habitat degradation and maintain food resources for orangutans in post-fire landscapes.
Co-Authors Al Hanif, Syivaul Jannah Firman . Hadi S Alikodra Hasan, Silvia Ike N. Nayasilana Lindayu, Feriska Liza Niningsih Priandani , Tasya Azzahra Saidatinah, Salsa Mumtaz Utami, Marcelita Putri Yeni A Mulyani