Garuda - Garba Rujukan Digital

Purwantara B

Unknown Affiliation

Author-ID : 238160

Veterinary

Published : 3 Documents Claim Missing Document

Claim Missing Document

Articles

Development of spermatogenic cells and sperm quality after administration of pegagan extract (Centella asiatica) M, Solihati; B, Purwantara; I, Supriatna; A, Winarto
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 3 (2013)
Publisher : Indonesian Animal Sciences Society

The purpose of this study was to determine the development of spermatogenic cells and sperm quality after administration extracts of pegagan (Centella asiatica) in various doses and duration of administration. The research was carried out with complete randomized design (CRD), consist of 16 combinations of dose and duration of treatment. Parameters measured consist of population of spermatogenic cells (spermatogonia, primary spermatocytes, late spermatids) and sperm quality (concentration, motility, abnormality). The data were processed using analysis of variance (ANOVA), and differences between treatments followed by Duncan test. The results show that both the dose and duration have very significantly (p < 0.01) affect on decreasing of late spermatid population, sperm motility and concentration, but not for population of spermatogonia, primary spermatocytes and sperm abnormalities. The decrease of population and quality may due to antifertility effect of pegagan, eventhough still in the normal range. It is concluded that spermatogenic cells development and sperm quality reduce after administration of pegagan extract, although infertility is not yet found up to the dose of 450 mg/kg BW for 49 days duration of administration. Key Words: Spermatogenic, Sperm Quality, Pegagan Extract

Development of spermatogenic cells and sperm quality after administration of pegagan extract (Centella asiatica) Solihati M; Purwantara B; Supriatna I; Winarto A
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 3 (2013): SEPTEMBER 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The purpose of this study was to determine the development of spermatogenic cells and sperm quality after administration extracts of pegagan (Centella asiatica) in various doses and duration of administration. The research was carried out with complete randomized design (CRD), consist of 16 combinations of dose and duration of treatment. Parameters measured consist of population of spermatogenic cells (spermatogonia, primary spermatocytes, late spermatids) and sperm quality (concentration, motility, abnormality). The data were processed using analysis of variance (ANOVA), and differences between treatments followed by Duncan test. The results show that both the dose and duration have very significantly (p < 0.01) affect on decreasing of late spermatid population, sperm motility and concentration, but not for population of spermatogonia, primary spermatocytes and sperm abnormalities. The decrease of population and quality may due to antifertility effect of pegagan, eventhough still in the normal range. It is concluded that spermatogenic cells development and sperm quality reduce after administration of pegagan extract, although infertility is not yet found up to the dose of 450 mg/kg BW for 49 days duration of administration. Key Words: Spermatogenic, Sperm Quality, Pegagan Extract

Microencapsulation of bull spermatozoa: Its viability in alginate-egg yolk media Kusumaningrum DA; Purwantara B; Yusuf TL; Situmorang P
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 1 (2015): MARCH 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Microencapsulation of spermatozoa is a process to entrap a number of spermatozoa in microcapsule. Alginate, as a natural polymer polysaccharide is commonly used in cell microencapsulation. Tris Yolk Citrate buffer is a good buffer for spermatozoa dilution, therefore this experiment aimed to determine optimal concentration of alginate and egg yolk to sperm quality in bull spermatozoa microencapsulation. Concentration of egg yolk and alginate in media of encapsulation were determined in applications of sperm microencapsulation. Four bulls were used as semen source and only semen with good quality were used in this study. Poolled semen was diluted using the medium to get final concentration 100 x 106 cell/ ml. The first study was conducted to determine the effect of concentration of alginate (0, 1, and 1.5%) on viability of spermatozoa. The second study to determine the effect of alginate concentration, egg yolk and its interaction was done by comparing two levels of alginate (1 and 1.5%) with four levels of egg yolk (5, 10, 15 and 20%). Viability of spermatozoa, motility (M), live spermatozoa (L) and Intact Apical Ridge (IAR) were observed at 0, 1, 2 and 3 h incubation at room temperature. Results indicated that alginate concentration increased the osmolality and viscosity but did not affect pH of the medium. The osmolality and viscosity of medium were 275, 325, 425 and 1.12, 26.62, 47.98 for concentration of alginate 0, 1 and 1.5% respectively. Percentage of motility is significantly lower (P<0.05) in alginate medium than those of control, and 1.5% alginate could produce more uniform beads. Concentration of alginate, egg yolk and its interaction did not significantly affect viability of sperm. It is concluded that the combination of 1.5% alginate with 5, 10, 15 or 20% egg yolk can be used as media for sperm encapsulation.

Title

Found 3 Documents
Search

Abstract

Abstract

Abstract

Title Search

Found 3 Documents Search

Abstract

Abstract

Abstract

Title

Found 3 Documents
Search