Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Ternak Veteriner
Kusumaningrum DA
Unknown Affiliation
Veterinary

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Female calves born resulted from an artificial inseminatioan (AI) using sexed sperm separated with egg albumin coloum p, Situmorang; RG, Sianturi; DA, Kusumaningrum; ., Ross; ., Maidaswar
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 3 (2013)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.483 KB) | DOI: 10.14334/jitv.v18i3.320

Abstract

An efforts to alter the sex ratio of calves born could be made by separating spermatozoa bearing X and Y chromosome. The object of this study is to increase the female dairy calves born resulted from artificial insemination using the sexed sperm. There were 2 activities conducted which were 1) Spermatozoa separation based on two egg albumin column with a concentration of 10% as the upper column (UC) and 30% as the lower column (LC) and 2) AI using semen from UC. Experiment designed was completely randomized designed with three separation time (10, 20 and 30 minutes) for the treatments. Semen from UC which is expected rich in spermatozoa X, diluted in Tris Citrate extender to give a final concentration of 100 million sperm/cc and frozen to - 196°C for AI purposes. Results showed that separation of semen using egg albumin column, affects the size of head of spermatozoa. The means of length, width and area size of head spermatozoa were 8.7 µm, 4.7 µm, 35.7 µm2 and 8.4 µm, 4.6 µm, 33.7 µm2 for UC and LC respectively. The livability of sperm from UC and LC were not statistically significant different. The mean percentage of motile (%M), live sperm (%L) and intact apical ridge (%IAR) were 77.8, 85.5, 78.1 and 76.1, 83.5, 78.4 for UC and LC respectively. Separation time did not significantly affect the size of spermatozoa heads of UC semen. The mean length, width and area size of head of spermatozoa were 8.7 µm, 4.5 µm, 36.1 µm2; 8.7 µm, 4.8 µm, 36.2 µm2 and 8.5 µm, 4.7 µm, 34.8 µm2 for 10; 20 and 30 minutes respectively. From total of 160 calves born showed that the female calves born was 65%. It is concluded that AI using sexed sperm separated with egg albumin could increase the percentage of female calve born. Key Words: Egg Albumin, Column, Separation Time, Size Sperm, Female Calve
Female calves born resulted from an artificial inseminatioan (AI) using sexed sperm separated with egg albumin coloum Situmorang p; Sianturi RG; Kusumaningrum DA; Ross .; Maidaswar .
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 3 (2013): SEPTEMBER 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.483 KB) | DOI: 10.14334/jitv.v18i3.320

Abstract

An efforts to alter the sex ratio of calves born could be made by separating spermatozoa bearing X and Y chromosome. The object of this study is to increase the female dairy calves born resulted from artificial insemination using the sexed sperm. There were 2 activities conducted which were 1) Spermatozoa separation based on two egg albumin column with a concentration of 10% as the upper column (UC) and 30% as the lower column (LC) and 2) AI using semen from UC. Experiment designed was completely randomized designed with three separation time (10, 20 and 30 minutes) for the treatments. Semen from UC which is expected rich in spermatozoa X, diluted in Tris Citrate extender to give a final concentration of 100 million sperm/cc and frozen to - 196°C for AI purposes. Results showed that separation of semen using egg albumin column, affects the size of head of spermatozoa. The means of length, width and area size of head spermatozoa were 8.7 µm, 4.7 µm, 35.7 µm2 and 8.4 µm, 4.6 µm, 33.7 µm2 for UC and LC respectively. The livability of sperm from UC and LC were not statistically significant different. The mean percentage of motile (%M), live sperm (%L) and intact apical ridge (%IAR) were 77.8, 85.5, 78.1 and 76.1, 83.5, 78.4 for UC and LC respectively. Separation time did not significantly affect the size of spermatozoa heads of UC semen. The mean length, width and area size of head of spermatozoa were 8.7 µm, 4.5 µm, 36.1 µm2; 8.7 µm, 4.8 µm, 36.2 µm2 and 8.5 µm, 4.7 µm, 34.8 µm2 for 10; 20 and 30 minutes respectively. From total of 160 calves born showed that the female calves born was 65%. It is concluded that AI using sexed sperm separated with egg albumin could increase the percentage of female calve born. Key Words: Egg Albumin, Column, Separation Time, Size Sperm, Female Calve
Microencapsulation of bull spermatozoa: Its viability in alginate-egg yolk media Kusumaningrum DA; Purwantara B; Yusuf TL; Situmorang P
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 1 (2015): MARCH 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1048.335 KB) | DOI: 10.14334/jitv.v20i1.1110

Abstract

Microencapsulation of spermatozoa is a process to entrap a number of spermatozoa in microcapsule. Alginate, as a natural polymer polysaccharide is commonly used in cell microencapsulation. Tris Yolk Citrate buffer is a good buffer for spermatozoa dilution, therefore this experiment aimed to determine optimal concentration of alginate and egg yolk to sperm quality in bull spermatozoa microencapsulation. Concentration of egg yolk and alginate in media of encapsulation were determined in applications of sperm microencapsulation. Four bulls were used as semen source and only semen with good quality were used in this study. Poolled semen was diluted using the medium to get final concentration 100 x 106 cell/ ml. The first study was conducted to determine the effect of concentration of alginate (0, 1, and 1.5%) on viability of spermatozoa. The second study to determine the effect of alginate concentration, egg yolk and its interaction was done by comparing two levels of alginate (1 and 1.5%) with four levels of egg yolk (5, 10, 15 and 20%). Viability of spermatozoa, motility (M), live spermatozoa (L) and Intact Apical Ridge (IAR) were observed at 0, 1, 2 and 3 h incubation at room temperature. Results indicated that alginate concentration increased the osmolality and viscosity but did not affect pH of the medium. The osmolality and viscosity of medium were 275, 325, 425 and 1.12, 26.62, 47.98 for concentration of alginate 0, 1 and 1.5% respectively. Percentage of motility is significantly lower (P<0.05) in alginate medium than those of control, and 1.5% alginate could produce more uniform beads. Concentration of alginate, egg yolk and its interaction did not significantly affect viability of sperm. It is concluded that the combination of 1.5% alginate with 5, 10, 15 or 20% egg yolk can be used as media for sperm encapsulation.
Co-Authors Maidaswar . Purwantara B Ross . Sianturi RG Situmorang p Yusuf TL