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Boy Bachtiar
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Dentistry

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QUANTIFICATION OF STREPTOCOCCUS SANGUINIS ISOLATED FROM DENTAL PLAQUE AND SALIVA OF SUBJECTS WITH AND WITHOUT CORONARY HEART DISEASE – ANALYSIS USING REAL-TIME PCR: KUANTIFIKASI STREPTOCOCCUS SANGUINIS YANG DIISOLASI DARI PLAK DAN SALIVA GIGI SUBYEK DENGAN DAN TANPA PENYAKIT JANTUNG KORONER DENGAN MENGGUNAKAN PCR REAL-TIME Nasution, Aini Hariyani; Kemal, Yulianti; Lessang, Robert; Bachtiar, Boy
Dentika: Dental Journal Vol. 20 No. 1 (2017): Dentika Dental Journal
Publisher : TALENTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.018 KB) | DOI: 10.32734/dentika.v20i1.645

Abstract

Coronary Heart Disease (CHD) is the major cause of death in most countries in the world. Gram-positive and Gram-negative bacteria have been identified in bacteremia cases and known to have a role in various vascular diseases, including Streptococcus sanguinis which is most frequently isolated from endocarditis patients and often associated with CHD. The purpose of this study was to analyze the number of Streptococcus sanguinis isolated from dental plaque and saliva of subjects with and without CHD. Bacterial colonies isolated from the dental plaque and saliva of 16 subjects without CHD and 8 subjects with CHD were planted in Mitis salivarius agar, and then the DNA was extracted and quantified with a Real-Time PCR technique using 16S rRNA specific primers. The quantification of Real-Time PCR showed that there was a difference in the number of S. sanguinis between the two groups of subjects, but an unpaired T-test showed that the difference was not statistically significant. Furthermore, the number of S. sanguinis from dental plaque in CHD subjects tends to be higher than that of non-CHD subjects whereas the number of S. sanguinis from saliva in non-CHD subjects tends to be higher than that of CHD subjects.
Phenotype and Genotype of Enterococcus faecalis Isolated from Root Canal and Saliva of Primary Endodontic Patients Mubarak, Zaki; Asmara, Widya; Wibawa, Tri; Bachtiar, Boy
Journal of Dentistry Indonesia Vol. 23, No. 1
Publisher : UI Scholars Hub

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Abstract

Objective: To investigate the phenotype and genotype of E. faecalis isolated from the root canal and saliva of primary endodontic patients with periapical lesions. Methods: Eighteen adult male and female individuals suffering from primary endodontic infection, either with or without periapical lesions, were involved in this study. Root canal scraping and saliva were collected from each subject and used for bacterial quantitation using a real-time polymerase chain reaction (RT-PCR). Enterococci were isolated using ChromAgar medium and then identified using both biochemical (Gram staining and catalase tests) and molecular biology (conventional PCR) methods. Gelatinase activity, polysaccharide capsul profile and mRNA ace expression level were determined using microbiological, biochemical and molecular biology approach, respectively. Genotype of E. faecalis was determined based on nucleotide sequence of ace and gelE genes analyzed using web-based 3730xl DNA Analyze software. Results: The results showed that except for its proportion, no significant difference was found in phenotypes (gelatinase activity and mRNA ace expression levels) and genotypes (polymorphism of Cps operon and variation of ace and gelE nucleotide sequences) of E. faecalis isolated from the root canal and saliva of primary endodontic patients had or had no periapical lesions. Conclusion: It can be concluded that E. faecalis proportion had a role in the occurrence of periapical lesions in the primary endodontic patients, but not gelatinase activity, mRNA ace expression level, Cps operon polymorphism or ace and gelE nucleotide sequence variations.
Co-Authors Nasution, Aini Hariyani Robert Lessang Tri Wibawa Widya Asmara Yulianti Kemal Yundari, Yundari