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All Journal Agroland : Jurnal Ilmu-Ilmu Pertanian Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrin : Jurnal Penelitian Pertanian
Bambang Hadisutrisno
Jurusan Perlindungan Tanaman, Fakultas Pertanian, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Journal : Agroland : Jurnal Ilmu-Ilmu Pertanian

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KARAKTERISASI PATOGEN HAWAR DAUN BAKTERI SECARA FENOTIPIK PADA BAWANG MERAH (ALLIUM CEPA L. KELOMPOK AGGREGATUM) Asrul Asrul; Triwidodo Arwiyanto; Bambang Hadisutrisno; Jaka Widada
Agroland: Jurnal Ilmu-ilmu Pertanian Vol 26, No 1 (2019)
Publisher : Universitas Tadulako

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Abstract

The research aimed at determining the types of pathogen associated with bacterial leaf blight diseases of shallots. The bacteria were isolated and characterized based on the  morphological, biochemical and physiological morphology of their colony and cell.  There were eight isolates of pathogenic bacteria from pure culture.  Generally, the isolates have Gram-negative characters, short rod-shaped cells, have peritrikus flagellum and mucoid, yellow or beige colonies, round, convex or flat forms, and is shiny. The isolates react positively to catalase, urease, levan, indole production, and tween 8 tests. They also can live at temperature between 20 – 370C, pH 5 – 7 and tolerant to NaCl content ranging from 0 – 8.5%. The isolates react negatively to oxidases, reduce nitrates, fluorescent pigments, arginine, gelatin and starch.  Based on these characteristics, the isolates found generally have a closer resemblance to the properties of P. ananatis with a similarity coefficient of 88%  than bacteria X. axonopodis pv. allii with a similarity coefficient of 78%.  The symptoms appeared in the plant leaves were wilted (water soaked), shrinking, curving down, chlorosis, necrosis, and dieback.
Co-Authors Asrul Asrul Bambang Hendro sunarminto Jaka Widada JAKA WIDADA La Ode Santiaji Bande Nur Prihatiningsih SUSAMTO SOMOWIYARJO Triwidodo Arwiyanto