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All Journal Agrin : Jurnal Penelitian Pertanian Agroista: Jurnal Agroteknologi Acta Solum
Jaka Widada
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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INOKULASI MIKORIZA DAN DISTRIBUSI ASIMILAT PADA TANAMAN KEDELAI Sri Suryanti; Didika Indradewa; Jaka Widada
AGROISTA : Jurnal Agroteknologi Vol. 1 No. 2 (2017): NOVEMBER
Publisher : Program Studi Agroteknologi INSTIPER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.308 KB) | DOI: 10.55180/agi.v1i2.15

Abstract

Soybean is a cheap source of vegetable protein. Effort to increase of soybean production have constrained the narrowness of fertile land. One way to increase soybean production in less fertile land is inoculation of mycorrhizal. The objective of this study are to conduct effect of mycorrhizal inoculation on physiological of soybean cultivars. The 18 x 2 factorial experiment was set up in completely randomize design using three replications during June to October 2013. The eighteen soybean cultivars (Grobogan, Burangrang, Sibayak, Kaba, Tanggamus, Anjasmoro, Argomulyo, Wilis, Panderman, Ijen, Baluran, Galunggung, Petek, Garut, Gepak Kuning, Malabar, Seulawah, Sinabung) were assigned as the first factor. The second factor was mycorrhizal inoculation with without mycorrhizal inoculation and mycorrhizal inoculation. Data were recorded for dry weight of shoot, dry weight of root, dry weight of plant, root shoot ratio at 56 days after planting, and harvest index at 84 days after planting. The result showed that mycorrhizal inoculation significantly increased the dry weight of shoot, dry weight of root, dry weight of plant and harvest index but significantly decreased of root shoot ratio.
SELEKSI MUTAN ANTIBIOSIS Bacillus subtilis B315 UNTUK PENGENDALIAN Ralstonia solanacearum Pr7 Nur Prihatiningsih; Triwidodo Arwiyanto; Bambang Hadisutrisno; Jaka Widada
Agrin Vol 18, No 1 (2014): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2014.18.1.213

Abstract

Bacillus subtilis B315 adalah bakteri antagonis terhadap patogen tanaman seperti Ralstonia solanacearum penyebab penyakit layu bakteri.  Salah satu mekanisme antagonisme adalah antibiosis.  Mutan antibiosis dibuat untuk membuktikan bahwa B. subtilis B315 mempunyai mekanisme antibiosis dalam mengendalikan R. solanacearum.  Tujuan penelitian adalah untuk menyeleksi mutan antibiosis B. subtilis B315 dibandingkan dengan B. subtilis B315 tipe alaminya, 2) mendeteksi sifat antibiosis dari  B. subtilis B315.  Metode yang digunakan adalah eksperimen melalui mutagenesis dengan EMS, seleksi mutan berdasarkan pengujian antibiosis, waktu generasi, pola nutrisi dan konsistensi koloni.  Sifat antibiosis dideteksi dengan ekstraksi metabolit sekunder.  Hasil penelitian menunjukkan bahwa mutagenesis dengan EMS yang optimum adalah pada waktu 274,7 menit dengan kematian maksimum 81,7%, Terdapat tiga kelompok mutan antibiosis yaitu yang tidak menghambat R. solanacearum, menghambat dengan zona hambatan 1-3 mm dan menghambat  dengan zona hambatan >3 mm. Mutan antibiosis terpilih yang kehilangan sifat  menghambat, namun waktu generasi dan pola nutrisi serta konsistensi koloni sama dengan B. subtilis B315 tipe alami adalah mutan M16.  Sifat antibiosis B. subtilis B315 ditunjukkan dengan metabolit sekunder yang diekstrak dengan metanol, menghasilkan puncak spot yang berbeda dengan mutan antibiosis M16. Kata kunci: Bacillus subtilis B315, mutan antibiosis, pengendalian, Ralstonia solanacearum  Pr7 ABSTRACT            Bacillus subtilis B315 is an antagonistic bacterium against plant pathogens such as Ralstonia solanacearum that causes bacterial wilt disease.  One of the antagonistic mechanisms is antibiosis. Antibiosis mutant is made to prove that B. subtilis B315 has an antibiosis mechanism in controlling R. solanacearum.  Aims of the research were 1) to select the B. subtilis B315 antibiosis mutant compared with B. subtilis B315 wild type, and  2) to detect antibiosis characters owned by B. subtilis B315.  The method used was an experiment through mutagenesis with EMS, mutant selection based on antibiosis test, generation time, nutrition pattern and colony consistency.  Antibiosis characters were detected by extraction of secondary metabolites.  Results of the research performed that optimal mutagenesis with EMS was at 274.7 minutes by maximum lethality of 81,7%.  There were 3 groups of antibiosis mutants i.e. not inhibiting R. solanacearum, inhibition with 1-3 mm of inhibiting zone, and inhibition with >3 mm of inhibiting zone.  The selected antibiosis mutant lost its inhibiting character, but the generation time and the nutrition pattern  and the colony consistency similar to B. subtilis B315 wild type was the M16 mutant.  Antibiosis characters of B. subtilis B315 were shown by secondary metabolites extracted with methanol to produce the peak spot that was different from the M16 antibiosis mutant. Key words: Bacillus subtilis B315, antibiosis mutant, control, Ralstonia solanacearum  Pr7 
Keragaman Jamur Mikoriza Arbuskular pada Rizosfer Tiga Varietas Tanaman Tebu: Diversity of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Three Sugarcane Varieties Zulfa Fatmawati; Jaka Widada; Donny Widianto; Anjar Cahyaningtyas; Devanda Ayu Lidya Permata Putri
Acta Solum Vol. 3 No. 2 (2025): Maret 2025
Publisher : Department of Soil, Faculty of Agriculture, Lambung Mangkurat University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/actasolum.v3i2.3293

Abstract

Arbuscular mycorrhizal fungi (AMF) form associations with more than 80% of the terrestrial plants. These associations enhance nutrient sequestration and plant resistance to environmental stress. The diversity of AMF is highly influenced by the host plants. The types of AMF in the Bululawang, Kidang Kencana, and Pasuruan Jengkol 922 sugarcane varieties grown in a similar location were identified through morphological and molecular methods. AMF spores were isolated by the wet sieving method. The morphological features of the AMF spore mounted in PVLG and Melzer’s reagent were observed under a microscope. The DNA of the fungi was extracted, and the ribosomal RNA genes were amplified by nested PCR with the NS1-NS4 primer pairs, followed by the AML1-AML2 primer pairs. A moderate diversity of AMF was observed, with a total of 269 spores found in the Kidang Kencana variety, 191 spores in the Bululawang variety, and 142 spores in the Pasuruan Jengkol 922 variety. Four AMF species have been identified morphologically and molecularly, namely Glomus flavisporum, Acaulospora koskei, Gigaspora Margarita, and Scutellospora savannicola. These findings indicate that the number and type of AMF associated with sugarcane roots are influenced by plant varieties, in which the Glomus sp. is the dominant species in the three sugarcane varieties studied.
Co-Authors Anjar Cahyaningtyas Bambang Hadisutrisno Devanda Ayu Lidya Permata Putri Didika Indradewa Donny Widianto Nur Prihatiningsih Sri Suryanti Triwidodo Arwiyanto Zulfa Fatmawati