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All Journal Lantanida Journal Jaringan Laboratorium Medis
Dhini Annisa Rahmasari Kanto
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Public Health

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KITOSAN DARI JAMUR TIRAM (Pleurotus ostreatus) DAN APLIKASINYA SEBAGAI ADSORBEN LOGAM KROM (Cr) Dhini Annisa Rahmasari Kanto; Riza Apriani; Mira Ilhami; Ai Eva
Lantanida Journal Vol 9, No 1 (2021)
Publisher : UIN AR-RANIRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/lj.v9i1.8335

Abstract

Oyster mushroom is one of the organisms that can be used as a chitosan source. Chitosan can be produced from chitin through deacetylation. As a chitin derivative compound, chitosan has immense application. In this study, chitin and chitosan were isolated from oyster mushrooms as much as 1.696% and 0.174% from 500 g of dried mushroom. The degree of deacetylation of chitosan is 80.70%. As a polycationic polymer, one of its applications is a metal adsorbent. Cr metal is a heavy metal that is mostly found in leather industrial waste. Chitosan is used as a Cr metal adsorbent to reduce Cr metal content in the solution. This study showed that there was a reduction of Cr metal content as much as 82.75%. Thus, chitosan isolated from oyster mushrooms in this study has the potential to be a bio-adsorbent in order to reduce Cr metal content in leather industrial waste.
Optimasi Variasi Konsentrasi Asam Trikloroasetat dalam Penanganan Sampel Ikterik pada Pemeriksaan Kadar Asam Urat Metode Uricase Sulthan Abyan Surya Durachman; Ani Riyani; Dhini Annisa Rahmasari Kanto; Dewi Nurhayati
Jaringan Laboratorium Medis Vol 8 No 1 (2026): May 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v8i1.14414

Abstract

Uric acid examination of the uricase method can be disturbed due to bilirubin in icteric serum. Bilirubin absorbs light at a similar wavelength to uricase reaction products, so it can cause gout results to appear higher than they actually are. To overcome this, proper pre-analytical handLing is needed with the addition of trichloroacetic acid (TCA). The purpose of this study was to determine the difference in uric acid levels before and after the addition of TCA to icteric serum and to determine the optimal concentration of TCA to reduce bilirubin disorders in uric acid examination. This research method uses a quasi-experiment using pooled sera that were recognized using catfish bile, with bilirubin levels of 2.13; 2.83; and 3.96 mg/dL. Each sample was treated with TCA at concentrations of 4%, 5%, and 6%. Uric acid levels were checked using the uricase method and data were analyzed using ANOVA and Post Hoc tests. The results showed a decrease in uric acid levels as TCA concentrations increased. The concentration of 6% TCA provided the most significant reduction, but the 5% TCA at bilirubin level of 2.83 mg/dL showed no significant difference compared to normal pooled sera (p > 0.05), suggesting that 5% TCA was the optimal concentration in the handLing of icteric samples on uric acid examination.
Co-Authors Ai Eva Ani Riyani Dewi Nurhayati Mira Ilhami Riza Apriani Sulthan Abyan Surya Durachman