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Study of morphological and physiological characteristics of the tolerant and sensitive mungbean genotypes to shading was carried out in the Station Research of the Indonesian Legume and Tuber Crops Research Institute (ILETRI) from September to December 2004. Nine tolerant genotypes (MMC 87 D-KP-2, MLG 369, MLG 310, MLG 424, MLG 336, MLG 428, MLG 237, MLG 429, and VC2768B) and three sensitive genotypes to shading (Nuri, MLG 460, and MLG 330) were tested in two shading levels, that were without sh TITIK SUNDARI
HAYATI Journal of Biosciences Vol. 16 No. 4 (2009): December 2009
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.16.4.127

Study of morphological and physiological characteristics of the tolerant and sensitive mungbean genotypes to shading was carried out in the Station Research of the Indonesian Legume and Tuber Crops Research Institute (ILETRI) from September to December 2004. Nine tolerant genotypes (MMC 87 D-KP-2, MLG 369, MLG 310, MLG 424, MLG 336, MLG 428, MLG 237, MLG 429, and VC2768B) and three sensitive genotypes to shading (Nuri, MLG 460, and MLG 330) were tested in two shading levels, that were without shading and shading of 52%. The randomized complete block design with three replications analysis. The results showed that leaf characters of shading tolerant and sensitive genotypes were different. The shading tolerant mungbean genotypes had good response to light stress so that the growth and development of the leaves were better than that of sensitive genotypes. The shading tolerant mungbean genotypes had bigger and thicker leaves than that of sensitive genotypes. The shading treatments caused reducing rate of PAR absorption, transpiration, photosynthesis, and CO2 stomata conductance. The reduction of all parameters in tolerant genotype was smaller than that of sensitive genotype. The specific leaf area at four weeks after planting could be used as shading tolerant indicator of mungbeans. Key words: mungbean, characteristics, morphology, physiology, leaves, tolerant, sensitive, shading

Tingkat Kritis Intensitas Cahaya Relatif Lima Genotip Kacang Hijau (Vigna radiatus L.) Titik Sundari; , Soemartono; , Tohari; W. Mangoendidjojo
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 3 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The aim of this experiment was to determine the critical relative light intensities (RLI) level of mungbean. The critical level was determined by 50% decreasing of grain yield. The experiment was conducted at the ILETRI (Indonesian Legume and Tuber Crops Research Institute), Malang, from February to May 2004. Five genotypes of mungbean, i.e. VC2768B, Kenari, Local Wongsorejo, Nuri and MLG 431 were grown in four relative light intensities (RLI) levels, respectively 100%, 75%, 50% and 25%, that were prepared before planting with artificial shading. The experimental design was a randomized complete block with three replicates. The results showed that reducing RLI increased specific leaf area, but decreased leaf number, leaf area, leaf, stem and root dry weight, as well as pod number, pod dry weight and grain yield per plant. Reducing RLI from 100% to 75%, 50% and 25% did result in 15%, 56% and 71% decreased grain yield of mungbean. The critical level of RLI on mungbean was 48% or was found at 52% shading. Key words: Vigna radiatus L., genotype, critical level, relative light intensities, growth, yield

Anatomi Daun Kacang Hijau Genotipe Toleran dan Sensitif Naungan Titik Sundari; , Soemartono; , Tohari; W. Mangoendidjojo
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The research to study the leaf anatomy of tolerant and sensitive mungbean genotypes to shading was conducted at Research Station of the Indonesian Legume and Tuber Crops Research Institute (ILETRI) from September to December 2004. Nine tolerant genotypes to shading including MMC 87 D-KP-2, MLG 369, MLG 310, MLG 424, MLG 336, MLG 428, MLG 237, MLG 429 and VC2768B and three sensitive genotypes including Nuri, MLG 460 and MLG 330 were tested in two shading levels, i.e., 0% (control) and 52% using randomized complete block design with three replications. The results showed that the leaves of tolerant genotypes have fewer thrichomes, thicker leaves, thinner epidermis cells, longer palisade tissues and greater number of stomata than those of sensitive genotypes when planted under shading condition. Key words: Mungbean, leaf anatomy, tolerant, sensitive, shading

Identifikasi Kesesuaian Genotipe Kedelai untuk Tumpang Sari dengan Ubi Kayu Titik Sundari; Siti Mutmaidah
Jurnal Ilmu Pertanian Indonesia Vol. 23 No. 1 (2018): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Intercropping is a modification of crop arrangements that can provide significant improvements in yields, with minimal labor investment. Increased productivity in soybean intercropping with cassava can be achieved through the use of suitable soybean genotypes for intercropping, as each genotype shows different responses to intercropping. The research aimed to identify the suitability of soybean genotypes for intercropping with cassava based on yield and land productivity assessed by the land equivalent ratio (LER). The research in 2016 was conducted at Kendalpayak Station Research, Malang, using a split plot design, repeated three times. The main plot is fifty-five soybean genotypes (50 promising lines and five control varieties, namely: Argopuro, Panderman, Dena 1, Dena 2, and Grobogan), and the sub plot is cropping systems (monoculture and intercropping). The results showed that intercropping of soybean with cassava caused soybean plants to experience shade stress, with shade rate of 62-90, 43-77, and 0-40% respectively for first, second and third-row positions. There are six soybean genotypes suitable for intercropping with cassava, Grob/Argom313-2, Grob/Pander395-3, Grob/Pander405-3, Grob/Pander428-1, Grob/Pander429-2, and Grob/IAC439-2, with an LER value of ≥1.8 which is a 10% selection limit to LER. Four combinations of them (Grob/Pander395-3, Grob/Pander405-3, Grob/Pander428-1, and Grob IAC439-2) provide an increase in cassava yields between 7.7-19.7% and reduction of soybean yields between 21.4-38.5%. While the two combinations other (Grob/Argom313-2 and Grob/Pander429-2) provide a reduction of cassava yields by 1.4 and 8.5% and reduction of soybean yield by 23.5 and 7.1%.

Pertumbuhan dan Hasil Beberapa Genotipe Kedelai pada Pola Tanam Baris Tunggal dan Baris Ganda Rina Artari; Titik Sundari
Buletin Palawija Vol 16, No 2 (2018): Buletin Palawija Vol 16 no 2, 2018
Publisher : Balai Penelitian Tanaman Aneka Kacang dan Umbi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bulpa.v16n2.2018.p74-83

Penelitian bertujuan untuk mengetahui respons beberapa genotipe kedelai terhadap pola pengaturan baris tanaman. Percobaan dilakukan di Kebun Percobaan (KP) Kendalpayak, Malang mulai Februari hingga Juni 2017, menggunakan rancangan split plot tiga ulangan. Petak utama adalah pengaturan pola baris, yaitu B1 (baris tunggal = 40 cm ×15 cm) dan B2 (baris ganda = 60 cm ×(20 cm ×15 cm), anak petak adalah 15 genotipe kedelai, terdiri dari 12 galur, dan tiga varietas (Dena 1, Dena 2, dan Grobogan) sebagai pembanding. Pemupukan 50 kg Urea + 150 kg SP36 + 150 kg KCl/ha dilakukan pada saat tanam. Pengendalian gulma dilakukan pada umur 2-4 minggu setelah tanam (MST). Pengamatan tinggi tanaman, jumlah cabang dan daun, diameter batang, luas daun, dan indeks klorofil daun umur 3, 5, 7, dan 9 MST dilakukan secara destruktif. Pada saat panen diamati jumlah polong isi dan polong hampa, dan bobot biji per tanaman. Hasil penelitian menunjukkan adanya perbedaan respons genotipe kedelai terhadap pengaturan pola baris tanaman. Genotipe IBK/Argop-276-3, Grob/Pander-395-2, dan Grob/IT-7-2 memberikan respons positif terhadap pola baris tunggal dengan hasil biji lebih tinggi dibanding ditanam dengan baris ganda. Genotipe Grob/Pander-397-6 dan Grob/Pander-428-1 memberikan respons positif terhadap pola baris ganda, dan mampu menghasilkan biji lebih tinggi daripada ditanam baris tunggal. Genotipe Grob/IT-7-5 memberikan hasil tinggi, baik pada pola baris tunggal maupun baris ganda.

Ubi Kayu Sebagai Bahan Baku Industri Bioetanol Erliana Ginting; Titik Sundari; Nasir Saleh
Buletin Palawija No 17 (2009): Buletin Palawija No 17, 2009
Publisher : Balai Penelitian Tanaman Aneka Kacang dan Umbi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bul palawija.v0n17.2009.p1-10

Ubi kayu sebagai bahan baku industri bioetanol. Penggunaan sumber energi alternatif terbarukan yang berasal dari hasil pertanian seperti bioetanol perlu dilakukan karena meningkatnya harga Bahan Bakar Minyak (BBM) di pasaran dunia dan menipisnya cadangan fosil. Ubi kayu cukup berpotensi sebagai bahan baku industri etanol karena mampu memproduksi etanol sebanyak 2.000–7.000 l/ha/th. Kandungan pati yang tinggi pada ubi kayu merupakan substrat yang baik untuk menghasilkan glukosa sebagai produk antara pada pembuatan etanol. Proses pengolahan ubi kayu menjadi etanol meliputi gelatinisasi pati, diikuti hidrolisis pati secara enzimatis menjadi glukosa dengan menggunakan enzim amilase dan glukoamilase (likuifikasi dan sakarifikasi), lalu difermentasi menjadi etanol dan dilanjutkan dengan distilasi dan dehidrasi untuk mendapatkan bioetanol dengan kadar 99,5% (fuel grade). Berdasarkan kadar gula total, pati dan ratio fermentasinya, beberapa varietas/klon ubi kayu, di antaranya CMM 99008-3, MLG 0311, OMM 9908-4 dan UJ-5 sesuai untuk bahan baku industri etanol dengan nilai konversi 4–4,5 kg umbi kupas segar/liter etanol 96%. Departemen Pertanian melalui program Peningkatan Mutu Intensisifikasi (PMI) dan perluasan areal tanam, telah memproyeksikan secara bertahap pengembangan ubi kayu untuk mendukung industri bioetanol. Program tersebut perlu mendapat dukungan semua stake holder, termasuk pengusaha/industri serta kebijakan serius dari Pemerintah untuk mendorong realisasi substitusi 10% premium dengan bioetanol (Gasohol E-10).

UJI INTEGRITAS BAHAN BAKAR NUKLIR BEKAS (BBNB) NOMOR RI-68, RI-190 DAN RI-187 Titik Sundari; Darmawan Aji; Arifin Arifin; Yhon Irzon; Marhaeni Joko Puspito; Lucia Kwin Pudjiastuti, S.K.M.
Reaktor : Buletin Pengelolaan Reaktor Nuklir Vol 13, No 2 (2016): Oktober 2016
Publisher : Direktorat Pengelolaan Fasilitas Ketenaganukliran (DPFK) RSG-GAS

ABSTRAK UJI INTEGRITAS BAHAN BAKAR NUKLIR BEKAS NOMOR RI-68, RI-190 DAN RI-187. Saat ini ada 245 bahan bakar nuklir bekas disimpan dengan tipe basah. Korosi galvanik antara kelongsong bahan bakar dan penyimpanan dalam rak stainless steel bisa terjadi meskipun karakteristik air kolam yang sangat baik juga akan menghambat terjadinya korosi. Korosi menyebabkan kebocoran pada bahan bakar bekas. Kegiatan uji integritas bahan bakar nuklir bekas dilakukan dengan metode uji cicip bahan bakar nuklir bekas. Uji cicip adalah metode tidak merusak yang digunakan untuk menguji bocornya bahan bakar bekas berdasarkan produk fisi yang terlepas dari kelongsong bahan bakar. Sistem uji cicip yang digunakan telah dipasang pada intermediate platform area kolam penyimpanan sementara bahan bakar nuklir bekas. Makalah ini membahas uji integritas bahan bakar nuklir bekas nomer identifikasi RI-68, RI-190 dan RI-187. Bahan bakar nuklir bekas yang diuji diambil dari rak kemudian dimasukkan ke dalam tabung uji cicip dan mengalami masa pengujian pada hari-1, ke-2, ke-3, ke-4, dan ke-8 dengan pengambilan sampel masing-masing sebanyak 500 ml air. Air uji masing-masing bahan bakar kemudian dianalisis kadar radionuklidanya menggunakan spektrometer gamma. Dari hasil analisis air uji cicip bahan bakar nuklir bekas nomor RI-68, RI-190, dan RI-187 ketiganya hanya terdeteksi radionuklida I-133 dan Sb-124 yang merupakan radionuklida yang sering terdeteksi pada air kolam. Tidak ditemukan radionuklida hasil belah lain maupun radionuklida yang terkandung dalam bahan bakar nuklir bekas pada ketiga BBNB tersebut sehingga dapat disimpulkan bahwa ketiga BBNB tersebut tidak terjadi kebocoran kelongsong bahan bakar atau dapat dikatakan mempunyai integritas yang baik.Kata Kunci: bahan bakar nuklir bekas, KH-IPSB3, analisis radionuklida, uji cicip. ABSTRACTINTEGRITY TEST OF SPENT NUCLEAR FUEL OF RI-68, RI-190 AND RI-187. Currently there are 245 bundles of spent nuclear fuel stored in the wet type. Galvanic corrosion between the fuel cladding and stainless steel storage rack can occur even though the characteristics of excellent water will also inhibit corrosion. Corrosion caused a leakage in the spent fuel. This integrity test for spent nuclear fuel is done by sipping test method for spent nuclear fuel. Sipping test is non-destructive method used to test the leaking of spent fuel based on fission product released from the cladding. This paper is aimed to discuss the integrity of spent nuclear fuel identification number of RI-68, RI-190 and RI-187. Sipping test system used has been installed at intermediate plat-form in interim storage area for spent nuclear fuel. Spent nuclear fuel being tested is taken from the rack and then these are put in a sipping tube. After experiencing a period of sipping on the 1st, 2nd, 3rd, 4th, and 8th day then they were do sampling each of 500 ml. Water sample each fuel was then analyzed using a gamma spectrometer to find the radionuclides contaminant. The result of the analysis show that the spent fuel number RI-68, RI-190, and RI-187 were not detected but only I-133 and SB-124. The two are often detected in the pond water. There were not found another fission product or radio nuclides contained in spent nuclear fuel so it can be concluded that the SNF do not leak or can be concluded that this three of SNF still have a good integrity.Keywords: spent nuclear fuel, TC-ISFSF, analysis of radionuclides, sipping test.

KESESUAIAN HASIL SWAB MENGGUNAKAN ALAT TCM (TES CEPAT MOLEKULER) DENGAN RT-PCR SEBAGAI GOLD STANDARD DIAGNOSTIK PEMERIKSAAN LABORATORIUM COVID-19 Pipit Sofiana; Christina Destri Wiwis Wijayanti; Halik Wijaya; Titik Sundari
Jurnal SainHealth Vol 6, No 1 (2022): Maret 2022
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v6i1.1728.17-21

Since the WHO announced the global pandemic caused by COVID-19 in February 2020, various steps have been taken to deal with it, one of which is increasing laboratory tests to support the diagnosis. This study aimed to describe the suitability of the COVID-19 swab results using the TCM (Molecular Rapid Test) tool with RT-PCR as the gold standard for diagnostic COVID-19 laboratory examinations at Dr. Wahidin Sudiro Husodo Hospital, Mojokerto City, reaching 100% or not. This research is descriptive with a retrospective approach, taking data from the examination results in August 2020, which were carried out at the Clinical Pathology Laboratory and PCR Biomolecular Laboratory, Dr. Wahidin Sudiro Husodo Hospital Mojokerto City. The results of the 20 samples examined using the TCM tool obtained postpositive results. In contrast,e results in as many as seven samples and 13 samples with negative results. In comparison, with the RT-PCR tool, 6 samples had positive results and 14 samples with negative results, so that 1 (5%) did not come according to the results, namely in samples with code C the results with the TCM tool showed p those with the RT-PCR tool showed negative results. Then the research results using the TCM if the percentage according to RT-PCR is 95%. Many factors could cause this difference in results. If viewed from pre-analytic factors, it can be caused by improper sampling process, patient cooperation, poor quality of the specimen or containing only very few samples, inaccurate sample delivery and storage conditions, sample contamination, and pipetting errors during sample preparation. At the analytical stage, the presence of cross-contamination, virus mutation, or PCR reaction barriers, the quality of the reagent kit, and the skills of the analyst working on it also affect the results because the more skilled you are, the more precise and correct standard procedures can be performed. Keywords: COVID-19, TCM (Molecular Rapid Test), RT-PCR

Pertumbuhan dan Hasil Beberapa Genotipe Kedelai pada Pola Tanam Baris Tunggal dan Baris Ganda Rina Artari; Titik Sundari
Buletin Palawija Vol 16, No 2 (2018): Buletin Palawija Vol 16 no 2, 2018
Publisher : Balai Penelitian Tanaman Aneka Kacang dan Umbi

Penelitian bertujuan untuk mengetahui respons beberapa genotipe kedelai terhadap pola pengaturan baris tanaman. Percobaan dilakukan di Kebun Percobaan (KP) Kendalpayak, Malang mulai Februari hingga Juni 2017, menggunakan rancangan split plot tiga ulangan. Petak utama adalah pengaturan pola baris, yaitu B1 (baris tunggal = 40 cm ×15 cm) dan B2 (baris ganda = 60 cm ×(20 cm ×15 cm), anak petak adalah 15 genotipe kedelai, terdiri dari 12 galur, dan tiga varietas (Dena 1, Dena 2, dan Grobogan) sebagai pembanding. Pemupukan 50 kg Urea + 150 kg SP36 + 150 kg KCl/ha dilakukan pada saat tanam. Pengendalian gulma dilakukan pada umur 2-4 minggu setelah tanam (MST). Pengamatan tinggi tanaman, jumlah cabang dan daun, diameter batang, luas daun, dan indeks klorofil daun umur 3, 5, 7, dan 9 MST dilakukan secara destruktif. Pada saat panen diamati jumlah polong isi dan polong hampa, dan bobot biji per tanaman. Hasil penelitian menunjukkan adanya perbedaan respons genotipe kedelai terhadap pengaturan pola baris tanaman. Genotipe IBK/Argop-276-3, Grob/Pander-395-2, dan Grob/IT-7-2 memberikan respons positif terhadap pola baris tunggal dengan hasil biji lebih tinggi dibanding ditanam dengan baris ganda. Genotipe Grob/Pander-397-6 dan Grob/Pander-428-1 memberikan respons positif terhadap pola baris ganda, dan mampu menghasilkan biji lebih tinggi daripada ditanam baris tunggal. Genotipe Grob/IT-7-5 memberikan hasil tinggi, baik pada pola baris tunggal maupun baris ganda.

Ubi Kayu Sebagai Bahan Baku Industri Bioetanol Erliana Ginting; Titik Sundari; Nasir Saleh
Buletin Palawija No 17 (2009): Buletin Palawija No 17, 2009
Publisher : Balai Penelitian Tanaman Aneka Kacang dan Umbi

Ubi kayu sebagai bahan baku industri bioetanol. Penggunaan sumber energi alternatif terbarukan yang berasal dari hasil pertanian seperti bioetanol perlu dilakukan karena meningkatnya harga Bahan Bakar Minyak (BBM) di pasaran dunia dan menipisnya cadangan fosil. Ubi kayu cukup berpotensi sebagai bahan baku industri etanol karena mampu memproduksi etanol sebanyak 2.000–7.000 l/ha/th. Kandungan pati yang tinggi pada ubi kayu merupakan substrat yang baik untuk menghasilkan glukosa sebagai produk antara pada pembuatan etanol. Proses pengolahan ubi kayu menjadi etanol meliputi gelatinisasi pati, diikuti hidrolisis pati secara enzimatis menjadi glukosa dengan menggunakan enzim amilase dan glukoamilase (likuifikasi dan sakarifikasi), lalu difermentasi menjadi etanol dan dilanjutkan dengan distilasi dan dehidrasi untuk mendapatkan bioetanol dengan kadar 99,5% (fuel grade). Berdasarkan kadar gula total, pati dan ratio fermentasinya, beberapa varietas/klon ubi kayu, di antaranya CMM 99008-3, MLG 0311, OMM 9908-4 dan UJ-5 sesuai untuk bahan baku industri etanol dengan nilai konversi 4–4,5 kg umbi kupas segar/liter etanol 96%. Departemen Pertanian melalui program Peningkatan Mutu Intensisifikasi (PMI) dan perluasan areal tanam, telah memproyeksikan secara bertahap pengembangan ubi kayu untuk mendukung industri bioetanol. Program tersebut perlu mendapat dukungan semua stake holder, termasuk pengusaha/industri serta kebijakan serius dari Pemerintah untuk mendorong realisasi substitusi 10% premium dengan bioetanol (Gasohol E-10).

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