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Soybean is a good source of protein. It has two major fractions, b-conglycinin (7S) and glycinin (11S). b-conglycinin’s function was known to suppress food intake, and this effect may be due to stimulating endogenous cholecystokinin (CCK) release. The aims of this study were to determine the highest content of total b-conglycinin and b-conglycinin sub unit-b level obtained from two varieties of soybean i.e. Wilis and Detam 1 varieties using different preparation and extraction methods. These two MEILINAH HIDAYAT; MUCHTAN SUJATNO; NUGRAHA SUTADIPURA; . SETIAWAN; AHMAD FARIED
HAYATI Journal of Biosciences Vol. 18 No. 1 (2011): March 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.1.37

Soybean is a good source of protein. It has two major fractions, b-conglycinin (7S) and glycinin (11S). b-conglycinin’s function was known to suppress food intake, and this effect may be due to stimulating endogenous cholecystokinin (CCK) release. The aims of this study were to determine the highest content of total b-conglycinin and b-conglycinin sub unit-b level obtained from two varieties of soybean i.e. Wilis and Detam 1 varieties using different preparation and extraction methods. These two soybean varieties were prepared into tempeh. Then the seed and tempeh were extracted using Deak and Panthee methods. There were six extracts analysed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) and coomassie brilliant blue (CBB) staining. The result was shown that Detam variety and raw seed contained the highest total b-conglycinin level. And Panthee method was the best method for extraction of total b-conglycinin, while Deak method was the best method for extraction of b-conglycinin subunit-b.

Muscle Mass Difference among Patients With Chronic Kidney Disease Stage 3 to Stage 5 Ivena Ivena; Rudi Supriyadi; Setiawan Setiawan
Althea Medical Journal Vol 6, No 1 (2019)
Publisher : Faculty of Medicine Universitas Padjadjaran

Background: Low muscle mass is one of the complications of chronic kidney disease (CKD), that may occur due to the accumulation of uremic toxins and other mechanisms related to CKD. The aim of this study was to explore the difference of muscle mass among CKD patients stage 3 to stage 5.Methods: A cross-sectional study was conducted with comparative analysis, using secondary data collected during the year 2017 by total sampling method. Subjects were patients aged over 18 years with CKD stage 3 to stage 5 from three hospitals in Bandung. Chronic Kidney Disease stages were determined based on Glomerular Filtration Rate. Muscle mass data was determined using Bioelectrical Impedance Analysis, collected from previous research. Data were analyzed usingone-way ANOVA or Kruskal-Wallis test.Results: One hundred subjects consisting of 57 men and 43 women were included in in this study with the mean muscle mass was 26.29%±4.86, of whom 67% of the patients had low muscle mass. There was no difference between male (27.21%±3.82) and female (28.36%±4.67). The muscle mass among patients withCKD stage 3 to stage 5 showed no significant results (p>0.05).Conclusions: There is no difference in muscle mass among CKD stage 3 to stage 5.

Pulmonary Function of Tuberculosis Patients in Medication at Dr. Hasan Sadikin General Hospital Bandung 2013–2014 Shalahuddin Galih Pradipta; Hendarsyah Suryadinata; Setiawan Setiawan
Althea Medical Journal Vol 4, No 3 (2017)
Publisher : Faculty of Medicine Universitas Padjadjaran

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Background: Tuberculosis (TB) is a dangerous global disease that already affects millions of people in the world and acts as the second most frequent cause of death in infectious diseases. Tuberculosis mostly attacks the lung and the inflammation process causes lung damage. The lung damage causes a decrease in pulmonary function. There has been no study about tuberculosis patient lung function in the advanced stage of medication.Methods: This study was conducted from August–October 2014 at DOTS Policlinic of Dr. Hasan Sadikin General Hospital Bandung. The study population was lung TB patients. The inclusion criteria were 1st category lung TB patients with anti-TB drug treatment on intensive phase. The exclusion criteria were extrapulmonary TB patients, patients with lung surgery history, and patients with asthma or chronic obstructive pulmonary disease (COPD). This study used purposive sampling. The subjects were given a spirometry test where the forced expiratory volume 1 second (FEV1), forced vital capacity (FVC), and their ratio were collected and then interpreted.Results: Among the 60 subjects included in this study, the data showed that 83.4% of the subjects had a decreased pulmonary function consisting of obstructive (6.7%) and restrictive patterns (76.7%).Conclusions: The majority of pulmonary TB patients treated with 1st category anti-TB drugs during intensive phase have a decrease in pulmonary function and most of them have restrictive pattern of pulmonary function.

Effect of Exercise Duration on Klotho and GluN2B Gene Expressions in Hippocampus of Wistar Rats with Moderate Intensity Exercise Hanna Goenawan; Bela Ita Karina; Titing Nurhayati; Julia Windi Gunadi; Setiawan Setiawan; Ronny Lesmana
Majalah Kedokteran Bandung Vol 52, No 3 (2020)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v52n3.2026

Exercise could delay the degeneration process by increasing Klotho, which is an endogen antioxidant. Klotho protects against oxidative damages, regulates hippocampal synaptic plasticity, and enhances GluN2B expression. This study aimed to analyze the effect of 14-day and 8-week moderate-intensity exercise on Klotho and GluN2B mRNA expressions in rat hippocampus. This study was an animal experimental study using Wistar rats that was performed from June to September 2019 at the Central Laboratory, Universitas Padjadjaran, Indonesia. Rats were divided into 4 groups: control (14-day control and 8-week control) and exercise group (14-day exercise and 8-week exercise). The exercise groups ran at a moderate intensity (20m/min) for 30min/day, 5x/week. Hippocampus was then subjected to klotho and GluN2B gene expression analysis. The exercise groups showed a higher Klotho expression, both in 14 days (0.844±0.124 vs. 1.057±0.013) and 8 weeks (0.897±0.072 vs. 1.380±0.168) although statistically insignificant (p=0.24). No difference in GluN2B gene expression was observed between control and exercise groups on 14 days (1±0.09 vs. 1.22±0.09) and 8 weeks (1.24±0.03 vs. 1.38±0.05; p=0.11). In conclusion, 14-day and 8-week moderate-intensity exercise did not alter Klotho and GluN2B expressions in rat hippocampus. Pengaruh Durasi Olahraga terhadap Perubahan Ekspresi Gen Klotho dan GluN2B di Hipokampus Tikus Galur Wistar yang Mendapatkan Perlakuan Lari Intensitas SedangOlahraga dapat menghambat proses degenerasi saraf dengan meningkatkan salah satu antioksidan endogen, yaitu Klotho. Klotho dapat melindungi sel neuron dari kerusakan oksidatif dan meningkatkan sinaptik plasticity pada hipokampus. Penelitian ini bertujuan untuk melihat pengaruh olahraga lari pada hewan coba pada 14 hari dan 8 minggu terhadap perubahan ekspresi gen Klotho and GluN2B di hipokampus. Penelitian ini merupakan penelitian eksperimental dilaksanakan pada bulan Juni hingga September 2019 di Laboratorium Sentral Universitas Padjadjaran. Penelitian ini menggunakan tikus jantan galur wistar. Tikus dibagi menjadi 4 kelompok perlakuan dan kontrol. Olahraga dilakukan dengan treadmill tikus, kecepatan 20m/min, 30 menit perhari selama 5 kali perminggu. Kemudian, mRNA otak digunakan untuk PCR dengan menggunakan primer klotho dan GluN2B. Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan yang bermakna (p=0.24) pada ekspresi gen Klotho pada 14 hari (0.844±0.124 vs 1.057±0.013) dan 8 minggu (0.897±0.072 vs 1.380±0.168) antara kelompok kontrol dan kelompok lari. Ekspresi gen dari GluN2B juga tidak menunjukkan perbedaan yang signifikan (p=0.11) pada 14 hari (1±0.09 vs 1.22±0.09) dan 8 minggu (1.24±0.03 vs 1.38 ± 0.05). Simpulan, bahwa 8 minggu olahraga tidak meningkatan ekspresi Klotho dan GluN2B yang signifikan

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