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All Journal HAYATI Journal of Biosciences ILMU KELAUTAN: Indonesian Journal of Marine Sciences Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Perikanan Universitas Gadjah Mada

Alim Isnansetyo

Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta

Author-ID : 2420346

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology

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Articles

Metode Antagonistik Langsung untuk Skrining Bakteri Laut Penghasil Senyawa Anti-methicillin-resistant Staphylococcus aureus (MRSA) Isnansetyo, Alim; Kamei, Yuto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Metode yang berbasis antagonistik langsung antara bakteri laut dan Staphylococcus aureus resistan metisilin (Methicillin-resistant Staphylococcus aureus, MRSA) berhasil digunakan untuk skrining bakteri laut penghasil senyawa anti-MRSA. Metode ini menggunakan medium agar lapis ganda untuk mendukung pertumbuhan bakteri laut dan MRSA. Beberapa bakteri laut penghasil senyawa anti-MRSA berhasil diisolasi dengan metode ini. Suatu bakteri laut, strain O-BC30 merupakan bakteri yang paling kuat menghambat pertumbuhan MRSA. Isolat-isolat bakteri yang didapat menunjukkan aktivitas yang stabil terhadap MRSA dan aktivitas ini juga konsisten dengan aktivitas ekstrak yang diperoleh dari sel bakteri yang dikultur pada medium agar. Metode ini sangat berguna dan efisien untuk skrining bukan hanya bakteri penghasil senyawa anti-MRSA tetapi juga bakteri penghasil antibiotik.

Independent Effects of Temperature, Salinity, Ammonium Concentration and pH on Nitrification Rate of the Ariake Seawater Above Mud Sediment ALIM ISNANSETYO; SAI GETSU; MASAHIRO SEGUCHI; MASUMI KORIYAMA
HAYATI Journal of Biosciences Vol. 21 No. 1 (2014): March 2014
Publisher : Bogor Agricultural University, Indonesia

The Ariake Sea located in the west parts of Kyushu Island is a semi-closed and macro-tidal shallow sea, and has the largest tidal flat in Japan. A large mud tidal flat with a productive ecosystem found along the western shoreline of the sea makes this area ideal as a major production site of nori (Porphyra yezoensis) in Japan. We determined the independent effect of temperature, salinity, ammonium concentration and pH on nitrification rates (NR) in the Ariake seawater above the mud sediment. The NR was determined by measuring accumulation of NO2-N production after adding sodium chlorate, an inhibitor of NO2-N to NO3-N oxidation. NRs were relatively high at 20-35 oC (optimum at 29.5 oC), but the rates were very low at 5, 10, and 40 oC. NRs increased sharply when increasing the salinity from 13 to 20 ppt, but it decreased drastically at salinity levels more than 35 ppt (optimum at 19 ppt). The relationship between ammonium concentration and NR showed a typical kinetic curve of enzymatic reaction with the maximum NR (Vmax) of 0.029 mM N.h-1 at 200 mM NH4-N (the half saturation constant (Ks) = 35 mM NH4-N). High NRs were determined at pH 7.5-8.0 (optimum pH 7.8). This is the first report on the independent effects of temperature, pH, salinity and NH4-N concentration on the NR of seawater, specifically the Ariake seawater.

Distribution of Ammonium-Oxidizing Bacteria in Sediment with Relation to Water Quality at the Musi River, Indonesia Melki Melki; Alim Isnansetyo; Jaka Widada; Murwantoko Murwantoko
HAYATI Journal of Biosciences Vol. 25 No. 4 (2018): October 2018
Publisher : Bogor Agricultural University, Indonesia

The Musi River is located in the southern Sumatra, Indonesia. Most of activities, i.e. agricultural, industrial, and urban activities are considered as being major sources of chemicals and nutrients with their waste products effluent into the river. Nitrification, the microbial oxidation of ammonia to nitrite and nitrate, occurs in a wide variety of environments and naturally remove anthropogenic N pollution. The purpose of this research was to determine of distribution of ammonium-oxidizing bacteria (AOB) in sediment with relation to water quality at the Musi river area. This study was conducted on rainy and dry season 2016 at five sampling sites from the freshwater to seawater at high and low tide conditions, the sampling sites are station St1 (Gandus), station St2 (Palembang city), station St3 (Upang), station St4 (Sungsang), and station St5 (Sea). Sediment samples were collected from the surface layer by using an Ekman grab. Some water quality such as salinity, temperature, pH, and dissolved oxygen (DO) were directly analyzed in the field, while other water quality such as NH4-N, NO2-N, and NO3-N were analyzed in the laboratory. The Density of AOB was determined by the most probable number of (MPN) method. The PCA was used to correlate variations of the AOB with physicochemical properties using software Xlstat. The results showed that the physicochemical properties had a range of salinity of 0 to 20 ppt, temperature of 29.21 to 31.82oC, pH of 4.88 to 7.93, DO of 3.44 to 11.33 mg/l, NH4-N in sediment of 0.04 to 0.87 mg/l, NO2-N in sediment of 0.01 to 1.77 mg/l, NO3-N in sediment of 0.09 to 2.08 mg/l. The density of AOB ranged from 7.2 x 102 to 6.1 x 103 cells/g sediment. Principal component analyses showed that temperature, pH, DO, and concentrations of nutrient contributed to density of AOB.

Characterizing the Three Different Alginate Type of Sargassum siliquosum Ervia Yudiati; Alim Isnansetyo
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 1 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

This research was aimed to identify the brown seaweed, to characterize the acid, sodium and calcium alginate, and to examine the alginate yield. The identification was done phaenotypically. The extraction method was pretreated by ethanol depigmentation, followed by the extraction of Na2CO3/EDTA and CaCl2 and presipitated with absolute ethanol. The characterization of alginate was done by FT-IR spectroscopy and Thin Layer Chromatography by comparing the samples with standard alginate (Sigma, USA). The key of identification showed that the species was Sargassum siliquosum. There are similarities in signal vibration and TLC spots among the samples and the standard. The TLC test was also showed that those alginates contain mannuronic and guluronic acid. The highest yield was produced by Sodium alginate (40,34% + 0,21), followed by Acid alginate (11,51% + 0,15) and Calcium alginate (4,8% + 0,09). Keywords: alginate, characterization, Sargassum siliquosum, yield

Isolation of Cellulolytic Bacterium Staphylococcus sp. JC20 from the Intestine of Octopus (Octopus sp.) for Fish Probiotic Candidate Indah Istiqomah; Alim Isnansetyo; Imelda Novita Atitus; Ahmad Fauzi Rohman
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 2 (2019)
Publisher : Universitas Gadjah Mada

Aim of this study was to isolate, characterize, and identify cellulolytic bacteria from the digestive tract of marine vertebrates and invertebrates as a candidate of fish probiotics. The bacteria were isolated from the digestive tract and grown on a cellulose agar plate. The bacteria were screened based on the cellulolytic activity, acid resistance, antagonist activity against fish pathogens, antibiotics sensitivity, ability to live in fish digestive tract and non-pathogenic test. Selected bacterium was identified molecularly, based on the 16S rDNA gene sequences, and phenotipically. A total of 14 bacteria demonstrated celulolitic index of 1.1-1.8. The bacteria with cellulolytic index of > 1.6 were screened by the selection criteria, resulted a selected strain, JC20 isolate which was isolated from the digestive tract of octopus (Octopus sp.). The selected bacterium was sensitive to antibiotics, resists to acidic environment, able to live in the fish digestive tract, and non-pathogen. Thus, the bacterium was potential for further characterization as fish probiotics candidate. Molecular and phenotypic identification revealed that JC20 isolate was Staphylococcus sp.

Co-Authors Ahmad Fauzi Rohman Ervia Yudiati Imelda Novita Atitus INDAH ISTIQOMAH JAKA WIDADA Kamei, Yuto MASAHIRO SEGUCHI MASUMI KORIYAMA Melki Melki Murwantoko . SAI GETSU

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