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All Journal HAYATI Journal of Biosciences Jurnal Pendidikan dan Pembelajaran Khatulistiwa (JPPK) Jurnal Perikanan Universitas Gadjah Mada Aquacultura Indonesiana Journal of Aquaculture and Fish Health SIGANUS: Journal of Fisheries and Marine Science Journal of Fisheries & Marine

INDAH ISTIQOMAH

Departemen Perikanan, Fakultas Pertanian, Universitas Gadjah Mada, Yogyakarta, Indonesia

Author-ID : 290084

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Other

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Growth, Total Production and Feed Efficiency of Catfish (Clarias sp.) Orally Administered with Shrimp Waste Hydrolyzate Hariyadi, Dimas Rizky; Isnansetyo, Alim; Istiqomah, Indah; Hardaningsih, Ign.; Wahyudi, Wahyudi; Kim, Sung Sam
Aquacultura Indonesiana Vol 19, No 1 (2018)
Publisher : Indonesian Aquaculture Society (MAI)

Shrimp Waste Hydrolyzate (SWH) /Shrimp Soluble Extract (SSE) is a product produced from the enzymatic process of shrimp waste. SWH contains essential and non-essential amino acids which useful for fish. This study aimed to determine effect of SWH on the growth and total production of catfish. The parameters observed were average growth rate, specific growth rate (SGR), food conversion ratio (FCR), protein efficiency ratio (PER), total production and length weight relationship from beginning until the end of rearing period. Growth performance was observed by calculating average growth rate and SGR of catfish. The results of this study showed that oral administration of SWH significantly increased SGR of catfish length at 4th sampling period with value 1.54% body length/day. Administration of SWH did not affect on the AGR, FCR, PER and total production. The growth pattern of catfish is negative allometric.

PENGARUH PENDAPATAN ORANG TUA TERHADAP MINAT SISWA MELANJUTKAN PENDIDIKAN KE PERGURUAN TINGGI Istiqomah, Indah; Genjik S, Bambang; BU, Bambang
Jurnal Pendidikan dan Pembelajaran Khatulistiwa Vol 5, No 5 (2016): Mei 2016
Publisher : Jurnal Pendidikan dan Pembelajaran Khatulistiwa

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstrak: Pendapatan orang tua merupakan semua pendapatan yang dapat dipergunakan oleh para penerimanya untuk membeli barang-barang dan jasa-jasa yang mereka inginkan. Pendapatan orang tua merupakan salah satu faktor yang mempengaruhi minat siswa melanjutkan pendidikan ke perguruan tinggi. Tujuan penelitian ini adalah untuk mengetahui apakah pendapatan orang tua dapat mempengaruhi minat siswa melanjutkan pendidikan ke perguruan tinggi. Metode yang digunakan adalah metode deskriptif. Data diambil dengan teknik komunikasi tidak langsung yaitu berupa angket dan teknik studi dokumenter. Teknik analisis data menggunakan regresi linear sederhana.Hasil penelitian menunjukkan bahwa pendapatan orang tua terhadap minat siswa melanjutkan pendidikan ke perguruan tinggi ditunjukkan dengan formulasi Y = 17,920 + 0,321X. Kontribusi R2=0,077 menyatakan bahwa pendapatan orang tua dalam meningkatkan minat siswa melanjutkan pendidikan ke perguruan tinggi sebesar 7,7% sedangkan sisanya sebesar 92,3% dipengaruhi oleh variabel lain. Â Kata Kunci : Pendapatan Orang Tua, Minat Â Abstract: Revenue parents are all revenues that can be used by recipients to buy goods and services they want. The income of parents is one of the factors that affect the interest of the students continue their education to college. The purpose of this study was to determine whether the parents' income can affect the interests of students continue their education to college. The method used is descriptive method. Data taken with the technique of indirect communication in the form of a questionnaire and documentary study engineering. Data were analyzed using linear regression sederhana.Hasil research shows that parents' income against the interest of students continue their education to higher education is shown by formulations Y = 17.920 + 0,321X. Contributions R2 = 0.077 states that the income of parents in improving student interest continuing education to higher education by 7.7% while the remaining 92.3% is influenced by other variables. Keywords: Income Parents, Interests Â

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.3.129

In vitro activity test is a critical evaluation to screen the potential biocontrol agent. We developed a selective medium for quantitative in vitro activity evaluation of bacterial biocontrol agents against pathogenic Vibrio in aquaculture. Sensitivity test of bacterial biocontrol and Vibrio spp. to nine antibiotics showed that oxytetracycline inhibited the growth of Vibrio spp., but did not inhibit the growth of the bacterial biocontrol. This selective inhibition activity of oxytetracycline suggested this antibiotic might be supplemented to establish a selective medium. The MIC of oxytetracycline against V. harveyi, V. parahaemolyticus, and V. algosus were 120, 250, and 120 mg/ml, respectively. These concentration did not inhibit the growth of bacterial biocontrols. Therefore, oxytetracycline was supplemented at 250 µg/ml in Zobell agar medium. This medium was used as a selective medium to enumerate the density of bacterial biocontrol. In vitro activity test of bacterial biocontrol (RLP1) against V. parahaemolyticus showed that strain RLP1 at density of 104 and 106 cells/ml was able to kill V. parahaemolyticus during 6 h incubation. At lower density, 102 cells/ml, this bacterial biocontrol agent was able to kill the pathogenic Vibrio during 12 h incubation. This study discovered a selective medium for the bacterial biocontrol and Vibrio spp. and provided the results of its application in the evaluation of in vitro activity of a bacterial biocontrol agent against V. parahaemolyticus. The results also revealed that strain RLP1 is a potential bacterial biocontrol against vibriosis in marine aquaculture.

Patogenisitas Vibrio Fluvialis 24SK terhadap Kerapu Tikus (Cromileptes altivelis) Indah Istiqomah; Alim Isnansetyo; Triyanto Triyanto; Kamiso Handoyo Nitimulyo; Muhammad Murdjani
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.159

This research was aimed to investigate the pathogenicity of Vibrio fluvialis 24SK in humpback grouper (Cromileptes altivelis) based on its Lethal Dosage 50 (LD50). V. fluvialis 24SK was isolated from ren of humpback grouper cultured in floating net cage at Brackishwater Aquaculture Development Center (BADC) Situbondo, with vibriosis signs. The bacterium was cultured in Tryptone Soy Broth (TSB) medium dissolved in trisalt solution (KCl, 0.75 g/l; MgSO4.7H2O; 14.2 g/l; NaCl, 18.4 g/l), incubated at 300C for 24 h. Infection was carried out by interperitoneal injection to humpback grouper (8-9 cm of total length) at 102, 104, 106, and 108 cfu/fish. Control fishes were injected with 0.2 ml trisalt solution. Disease sign and mortality of fishes were observed every eight hour for 40 days. LD50 was calculated based on Dragstedt Behrens method (Hubert, 1980). Result indicated that infection of the bacteria at 106 and 108 cfu/fish caused sub-acute disease signs, such as haemorhagic on operculum, base of fins (pinnae pectorales, pinnae abdominales, pinna analis), and also head and abdomen, while infection at 102 and 104 cfu/fish caused chronic disease signs, such as haemorhagic on fins base which was followed by necrotic on fins and skin tissue in prolonged time. Histopathologically, infection of the bacteria caused atrophy on the gills, infiltrations of lymphocyte, heterofel and plasma cell on the gills and fins base, vacuolar degeneration on the liver, and also present the bacteria colony on the fins base and intestine tissues. V. fluvialis 24SK has LD50 at (1,1±0,5)x107 cfu/fish.

ISOLASI, IDENTIFIKASI DAN KARAKTERISASI Vibrio spp. PATOGEN PENYEBAB VIBRIOSIS PADA KERAPU DI BALAI BUDIDAYA AIR PAYAU SITUBONDO Kamiso Handoyo Nitimulyo; Alim Isnansetyo; Triyanto Triyanto; Indah Istiqomah; Muhammmad Murdjani
Jurnal Perikanan Universitas Gadjah Mada Vol 7, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9053

This research was conducted to isolate, identify, and characterize pathogenic Vibrio spp., causative agents of vibriosis in grouper at Brackishwater Aquaculture Development Center (BADC), Situbondo. Twenty-nine isolates were isolated from wound, gills, eyes, liver, and ren of grouper with vibriosis on Thiosulphate Citrate Bile Sucrose Agar (TCBSA) medium. Koch Postulate Test was conducted to determine pathogenic Vibrio spp., by intraperitoneal injection to humpback grouper (Cromileptes altivelis) (9-10 cm of total length) at 106 cells/fish in triplicates. Results indicated that the pathogenic Vibrio spp., causative agents of vibriosis in grouper at BADC, Situbondo were identified to be V. alginolyticus, V. anguillarum, V. metchnikovii, V. vulnificus, V. fluvialis, V. furnisii, and V. parahaemolyticus. Infection of the pathogenic Vibrio spp. caused acute mortality within 17-46 hours with specific disease signs like haemorhagic on fins (pinnae pectorales, pinnae abdominales, pinna analis) and also on the body. These results suggested that vibriosis should be monitored and controlled properly and quickly.

ISOLASI, KARAKTERISASI, DAN PATOGENITAS BAKTERI PENYEBAB PENYAKIT PADA GURAMI (Osphronemus goramy) DI KABUPATEN BANTUL Murwantoko Murwantoko; Rozi Rozi; Indah Istiqomah; Kamiso H. Nitimulyo
Jurnal Perikanan Universitas Gadjah Mada Vol 15, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9102

Gouramy (Osphronemusgoramy) is an important ﬁsh comodity and preferred to be consumed by people. One of the limiting factor in freshwater aquaculture is bacterial infections. The objective of this research was to identify the species of pathogenic bacteria on gourami from Bantul Regency and to determine their pathogenicity. Eighteen diseased gouramy were collected from seven different ﬁ sh farms. Bacteria were isolated from kidney and purifyon TSA medium. Characterization was conducted on the morphology of bacterial colonies and cells, biochemical tests. Koch Postulate Test was done to conﬁrm pathogenic properties of the isolated bacteria. Pathogenicity test was performed by intraperitoneal injection of a serial doses of bacteria into gouramy and the Lethal Dosage 50 (LD50) was calculated. Twenty isolates of Aeromonas hydrophila, 5 isolates of Aeromonas salmonicida and one isolate of Vibrio cholerae,Vibrio anguillarum and Pseudomonas ﬂuorescens respectively were isolated from and conﬁ rmed as pathogen ingouramy. Pathogenic diversity was found among isolated bacteria, which a group of virulent bacteria as Aeromonas hydrophila GBNB, GGRB, and GCMA3 with LD50 values of 6.28 x 105, 2.09 x 105 and 5.37 x 105 CFU /ﬁsh; and a group of non virulent bacteria as A. hydrophila GKRA isolate and Vibrio cholera GPDA isolat with LD50 value of 7.14 x 107 and 3.45 x 107 CFU/ﬁsh respectively.

Toxicity Test for Evaluating Food Safety of New Edible Seaweeds, Enteromorpha sp. and Laurencia sp. Alim Isnansetyo; Indah Istiqomah; Retno Widaningroem; Triyanto Triyanto; Rina Anggraeni Safia; Riesa Yosita; Senny Helmiati
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 1 (2019)
Publisher : Universitas Gadjah Mada

Seaweeds are potential foodstuff and source of bioactive substances that are very useful in the food and pharmaceutical industries. The objective of this study was to evaluate toxicity of aqueous extracts of seaweeds by Brine shrimp lethality test (BLT) and cytotoxicity test. The seaweeds used in this study were Enteromorpha sp. and Laurencia sp. collected from the Gunungkidul coastal line. The cytotoxicity test was performed against a normal cells line of Vero cells and a cancer cells line of HeLa cells. The cells lines were cultured in M119 medium with addition of 10% FBS and incubated at 37°C in CO2 incubator until confluent. Cells were harvested and cultured in 96 microplate with an initial density of 2x104 cells / 100 ml / well. The cells lines were treated with seaweed extracts at 24 hours after incubation. After additional 24 h incubation, the cell proliferation was observed by MTT method (3- [4,5-dimethylthiazol-2-yl] -2,5- diphenyltetrazolium bromide.The results showed that the two aqueous extract were not toxic in the BLT test, and did not exhibited any cytotoxicity activity against HeLa and Vero cells lines. Concentration-dependent cell proliferation was also not found. This results indicated that Enteromorpha sp. and Laurencia sp. extracts were no cytotoxic and might be consumed safely. These overall results suggested that the two seaweeds might be developed to be a new foodstuff for humans.

Isolation of Cellulolytic Bacterium Staphylococcus sp. JC20 from the Intestine of Octopus (Octopus sp.) for Fish Probiotic Candidate Indah Istiqomah; Alim Isnansetyo; Imelda Novita Atitus; Ahmad Fauzi Rohman
Jurnal Perikanan Universitas Gadjah Mada Vol 21, No 2 (2019)
Publisher : Universitas Gadjah Mada

Aim of this study was to isolate, characterize, and identify cellulolytic bacteria from the digestive tract of marine vertebrates and invertebrates as a candidate of fish probiotics. The bacteria were isolated from the digestive tract and grown on a cellulose agar plate. The bacteria were screened based on the cellulolytic activity, acid resistance, antagonist activity against fish pathogens, antibiotics sensitivity, ability to live in fish digestive tract and non-pathogenic test. Selected bacterium was identified molecularly, based on the 16S rDNA gene sequences, and phenotipically. A total of 14 bacteria demonstrated celulolitic index of 1.1-1.8. The bacteria with cellulolytic index of > 1.6 were screened by the selection criteria, resulted a selected strain, JC20 isolate which was isolated from the digestive tract of octopus (Octopus sp.). The selected bacterium was sensitive to antibiotics, resists to acidic environment, able to live in the fish digestive tract, and non-pathogen. Thus, the bacterium was potential for further characterization as fish probiotics candidate. Molecular and phenotypic identification revealed that JC20 isolate was Staphylococcus sp.

Protective Effect of Microbubble Aeration and Dietary Probiotics BALSS on Survival and Immunity of White Leg Shrimp (Litopenaeus vannamei) Postlarvae against Acute Low Salinity Stress Shima Bhaskara Ayuningrum; Indah Istiqomah; Rustadi Rustadi; Bambang Triyatmo; Alim Isnansetyo; Wiratni Budhijanto; Deendarlianto Deendarlianto
Jurnal Perikanan Universitas Gadjah Mada Vol 22, No 1 (2020)
Publisher : Universitas Gadjah Mada

Survival rate and immunity of white leg shrimp ((Litopenaeus vannamei) postlarvae against acute salinity stress were evaluated in indoor experimental trials. The research was conducted with 2x2 factorial design with two levels of aeration (microbubble and macro bubble) and two levels of diet (with and without probiotics) resulting four treatments in triplicate: application of microbubble with gut probiotics (MiP), without gut probiotics (MiC), and macro bubble with gut probiotics (MaP) and without probiotics (MaC). White leg shrimps measuring 7.6 g were maintained in the tanks for 60 days and fed five times a day at a dose of 5% biomass. Harvested shrimp were tested for low salinity stress (5 ppt) for 3 hours. Glucose level, total protein, bactericidal activity, natural agglutination, phenoloxidase, respiratory burst, and superoxide dismutase activities in the hemolymph and the shrimp survival rate (SR) were analyzed before and after the stress tests. Although all examined parameters remained the same between groups before the stress test, significant differences were found among them after the stress test. Microbubble aeration obtained stabilization of hemolymph glucose level, best survival rate, bactericidal activity, and agglutination activities. No significant effect was resulted by the dietary probiotic treatments, but the interaction with aeration treatments revealed the importance to compensate shrimp survival rate when microbubble aeration is unavailable.

Struktur Komunitas Plankton pada Air Kolam Ikan Lele yang Berbeda Warna Karunia Adetera Nungki Wijayanti; Murwantoko Murwantoko; Indah Istiqomah
Jurnal Perikanan Universitas Gadjah Mada Vol 23, No 1 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.62733

Plankton dalam perairan memiliki peranan yang penting sebagai produsen serta penyuplai nutrisi maupun indikator lingkungan. Struktur komunitas dan faktor pendukung lingkungan berpengaruh terhadap keadaan ekosistem perairan budidaya serta pengaruhnya pada komoditas budidaya. Penelitian ini membahas mengenai struktur komunitas plankton pada air kolam lele yang berbeda warna. Kolam yang digunakan adalah kolam budidaya lele air hijau di Kalasan, cokelat di Prambanan, dan merah di Berbah, Sleman. Parameter kualitas air yang meliputi pH, suhu, bahan organik, dan oksigen terlarut diukur sebanyak 3 kali setiap 2 minggu sekali dan kadar nitrat, nitrit, amonia diukur pada saat awal sampling. Pengambilan sampel plankton dilakukan dengan menggunakan plankton net sebanyak 3 kali setiap 2 minggu sekali. Jenis dan jumlah plankton diamati secara mikroskopis. Penentuan jenis plankton dilakukan dengan membandingkan ciri-ciri yang tampak dengan literatur. Dominansi plankton, diversitas plankton, kepadatan serta pengaruh kualitas air terhadap struktur komunitas plankton dianalisis secara statistik. Hasil pegamatan menunjukan plankton yang mendominansi adalah Chlorophyta dengan nilai indeks diversitas maupun dominansi di semua jenis kolam tergolong rendah-sedang. Berdasarkan uji statistik menunjukkan kualitas air yang berpengaruh terhadap struktur komunitas plankton adalah bahan organik, amonia, dan nitrit. Struktur komunitas plankton pada air kolam berbeda warna menunjukkan terdapat 15 filum yang terdiri dari 32 kelas. Plankton yang dominan pada kolam warna air hijau adalah Microcystis aeruginosa, sedangkan kolam cokelat dan merah adalah Chlorella varigatus. Nilai diversitas dan dominansi pada ketiga warna air cenderung stabil moderat, karena termasuk dalam kategori sedang yang menunjukan kondisi kolam tergolong stabil dan baik untuk budidaya.

Co-Authors . MUHTADI . TRIYANTO Abdullah, Faisal Zain Ahmad Fauzi Rohman Alim Isnansetyo Alim Isnansetyo Alim Isnansetyo Alim Isnansetyo Alim Isnansetyo ALIM ISNANSETYO Alim Isnansetyo Alim Isnansetyo ALIM ISNANSETYO Amir Husni Andita Ratih Dewanti Anggi Octari Putri ANIK KUSMIATUN Arsana, I Made Putra Bambang BU Bambang Genjik S Bambang Triyatmo Buana Basir Deendarlianto Hariyadi, Dimas Rizky Ign. Hardaningsih Imelda Novita Atitus Ismail, Norshida Jabbar, Firmansyah Bin Abd Kamiso H. Nitimulyo Kamiso Handoyo Nitimulyo Kamiso Handoyo Nitimulyo KAMISO HANDOYO NITIMULYO Karunia Adetera Nungki Wijayanti Kim, Sung Sam Latifah, Ma'rufa Nurul Muhammad Murdjani Muhammmad Murdjani Murti, Fauziah Arelia Usmiara Murwantoko . Murwantoko Murwantoko Muwantoko Murwantoko Nikmah, Nur Lailatul Fitrotun Retno Widaningroem Riesa Yosita Rina Anggraeni Safia Rozi Rozi Rustadi Rustadi Senny Helmiati Senny Helmiati Shima Bhaskara Ayuningrum Syakuri, Hamdan Toni Kuswoyo Triyanto Triyanto Triyanto Triyanto Triyanto Triyanto Wahyudi Wahyudi Wiratni Budhijanto Yasa, Ngurah Sedana

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