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All Journal HAYATI Journal of Biosciences Jurnal Respirologi Indonesia
Eric Hayes
Porsolt SAS
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Health Professions Medicine & Pharmacology

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Isolation and Characterization of C-C Chemokine Ligand 7 (CCL7) in Cynomolgus Macaques Sela S. Mariya; Fitriya N. Dewi; Villiandra Villiandra; Yasmina A. Paramastri; Diah Iskandriati; Uus Saepuloh; Eric Hayes; Joko Pamungkas; Dondin Sajuthi
HAYATI Journal of Biosciences Vol. 26 No. 3 (2019): July 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.18 KB) | DOI: 10.4308/hjb.26.3.129

Abstract

Cynomolgus macaques (Macaca fascicularis) are an established animal model of asthma, which exhibit different responses to allergen exposure that are clinically relevant. The chemokine ligand gene (CCL7) encodes Monocyte Chemotactic Protein-3, which has an important role in asthma pathogenesis. While CCL7 polymorphism in humans is associated with asthma phenotype, very little is known about CCL7 in nonhuman primate models of respiratory disease. The objective of this study was to isolate and characterize CCL7 gene in cynomolgus macaques of Indonesian origin. In this study, we used sequencing and bioinformatics technique for gene isolation, characterization, and protein 3D structure prediction. We isolated a 2253 base-pair (bp) sequence of CCL7 in cynomolgus macaques, which exhibited 95% similarity in coding sequence to human CCL7. The amino acid sequence was more closely clustered with human CCL7 than with that of rodents. Importantly, the predictive protein structure of CCL7 was similar to that in humans. These similarities in CCL7 suggests the potential of cynomolgus macaque as a translational model to study asthma, particularly in the context of genetics and role of chemokines such as CCL7.
CCL2 and CCR2 Expression in Broncoalveolar Lavage Fluid of Cynomolgus Macaque Model Of Asthma Sela S Mariya; Fitriya N Dewi; Villiandra Villiandra; Yasmina A Pramastri; Diah Iskandriati; Eric Hayes; Joko Pamungkas; R.P Agus Lelana; Ligaya I Tumbelaka; Dondin Sajuthi
Jurnal Respirologi Indonesia Vol 38, No 2 (2018)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v38i2.166

Abstract

Background: Animal models are essential for the development and improvement of novel and effective methods for diagnostic and treatment of human diseases. Cynomolgus monkeys have been used as animal model in asthma studies wherein they exhibited different responses to allergen exposure in the airway; some were low responders while others were high responder. CCL2 is a potent chemotactic factor for monocytes and the gene expression was high in animal model of asthma. The aim of this study was to evaluate CCL2 and CCR2 expression between the low and high responders. Methods: Realtime PCR technique was used to evaluate CCL2 and CCR2 gene expression in bronchoalveolar lavage samples. The subject of this study was asthmatic cynomolgus monkeys, consisted of 4 low responders and 4 high responders. This study was held in Biotechnology Laboratory of Primate Research Center LPPM IPB on October 2016- January 2017. Results: CCL2 and CCR2 expression in low responders were higher than those in high responders at 24hours post airway challenge. Conclusion: CCL2 may potentially be developed as target for therapy or a genetic marker for asthma responsiveness in individuals. (J Respir Indo 2018; 38(2): 115-22)
Co-Authors Dewi, Fitriya Nur Annisa Diah Iskandriati Diah Iskandriati Dondin Sajuthi Pamungkas, Joko R.P Agus Lelana Sela S Mariya Sela S. Mariya Uus Saepuloh Villiandra Villiandra Villiandra Villiandra Yasmina A Pramastri Yasmina A. Paramastri