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All Journal HAYATI Journal of Biosciences Caraka Tani: Journal of Sustainable Agriculture
Windri Handayani
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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The Development of Gametophyte Sterilization Method for Liverworts Acrolejeunea fertilis (Reinw., Blume and Nees) Schiffn. In vitro Culture Mouleidi Dwi Putri; Windri Handayani; Astari Dwiranti; Andi Salamah; Niarsi Merry Hemelda; Afiatry Putrika
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.39

Abstract

In vitro culture gametophytes of leafy liverworts often have problems in their sterilization process. These problems due to the high level of contamination and the fragile structure of the gametophyte leafy liverworts. The structures can be easily to damage after exposure to disinfectant. This study aimed to observe the concentration and the exposure time of “Bayclin” commercial bleach to suppress contamination with the viability of Acrolejeunea fertilisgametophytic explants. This research was conducted using control and 6 combination treatments with “Bayclin” concentration (1.00%, 1.25%, and 1.50%) and exposure time 60 and 120 seconds, then accompanied by the addition of Tetracycline 2.5 mg/ml. The qualitative parameters observed were the explant color, the type and location of contamination, and the growth of explants. The quantitative parameters were the percentage of contamination, the percentage of growth, and the number of new branches. The results showed that “Bayclin” 1.25% and 1.50% with 60 seconds exposure time has the lowest percentage of contamination which is 70% until the 7th days after planting. The most common type of internal contamination from the explant is bacteria and fungi. However, the growth of the new branch still occurs in some explants even though it has been contaminated and browned.
The Effect of Silver Nanoparticles Stabilized with Tannic Acid for Nano-Priming on Zea mays L. Seeds Germination Windri Handayani; Richard Owen Tanadi; Aminah Umar
Caraka Tani: Journal of Sustainable Agriculture Vol 40, No 1 (2025): January
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v40i1.85977

Abstract

Silver nanoparticles (AgNPs) have various benefits for application in the agricultural sector, such as nano-seed priming to enhance seedling growth and development. In this research, the effectiveness of AgNPs sizes and concentration to enhance Zea mays seeds germination has been investigated. The AgNPs were synthesized using various concentrations of tannic acid (0.025, 0.25, and 5 mM) to produce AgNPs with different sizes to know their optimum size and concentration. The synthesized AgNPs were characterized using a UV-Vis spectrophotometer to determine the absorption spectrum of AgNPs within 400 to 500 nm. Besides that, a transmission electron microscope (TEM) was used to determine the size and shape of the AgNPs, and an atomic absorption spectrophotometer was used to determine the concentration. The results show AgNPs with sizes of 13.39±2.40, 27.25±4.09, and 46.7±10.75 nm, respectively. Subsequently, AgNPs with concentrations of ~8, ~16, and ~24 mg l-1 were exposed to Z. mays seeds for 24 hours, then germinated for 14 days. The results revealed that AgNPs with a size of ~27 nm and a concentration of ~24 mg l-1 showed the highest germination rate and growth despite the control and other treatments. This indicates that the AgNPs with those properties have the potential as a seed nano-priming agent.
Co-Authors Aminah Umar Andi Salamah Dwiranti, Astari Mouleidi Dwi Putri Niarsi Merry Hemelda Putrika, Afiatry Richard Owen Tanadi