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The Development of Gametophyte Sterilization Method for Liverworts Acrolejeunea fertilis (Reinw., Blume and Nees) Schiffn. In vitro Culture Mouleidi Dwi Putri; Windri Handayani; Astari Dwiranti; Andi Salamah; Niarsi Merry Hemelda; Afiatry Putrika
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.39

Abstract

In vitro culture gametophytes of leafy liverworts often have problems in their sterilization process. These problems due to the high level of contamination and the fragile structure of the gametophyte leafy liverworts. The structures can be easily to damage after exposure to disinfectant. This study aimed to observe the concentration and the exposure time of “Bayclin” commercial bleach to suppress contamination with the viability of Acrolejeunea fertilisgametophytic explants. This research was conducted using control and 6 combination treatments with “Bayclin” concentration (1.00%, 1.25%, and 1.50%) and exposure time 60 and 120 seconds, then accompanied by the addition of Tetracycline 2.5 mg/ml. The qualitative parameters observed were the explant color, the type and location of contamination, and the growth of explants. The quantitative parameters were the percentage of contamination, the percentage of growth, and the number of new branches. The results showed that “Bayclin” 1.25% and 1.50% with 60 seconds exposure time has the lowest percentage of contamination which is 70% until the 7th days after planting. The most common type of internal contamination from the explant is bacteria and fungi. However, the growth of the new branch still occurs in some explants even though it has been contaminated and browned.
The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp. Anna Widyastuti; Afiatry Putrika; Astari Dwiranti; Andi Salamah; Niarsi Merry Hemelda; Windri Handayani
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.110

Abstract

In vitro cultures of leafy liverworts are still facing significant challenges due to high-level of explant contamination. The sterilization process can easily damage the structure of liverwort after exposure to the disinfectant. This study was to determine the concentration and time exposure of commercial bleach as a disinfectant to suppress contamination using the gametophyte culture of Lopholejeunea sp. The experiment consisted of control and six treatment combinations of commercial bleach with concentration 0.5, 0.75, and 1% (v/v), and exposure time (60 and 90 seconds). The type and location of contamination, the color of the explants after sterilization, and response after 30 days were observed. The results showed that the 0.75% bleach with 60 and 90 seconds exposure time had a lower contamination until the 7th day of culture. The most common type of contamination is bacteria and fungi that arise from the explant. Despite the contamination, it did not inhibit shoot formation. Further studies still needed to determine the type of fungicides and antibiotics with the most potent concentration and exposure time should be tested to obtain an axenic and viable culture of liverworts Lopholejeunea sp.
PEMANFAATAN LEBAH DAN PENGELOLAAN SAMPAH UNTUK EKONOMI RUMAH TANGGA YANG BERKELANJUTAN Afiatry Putrika; Deti Purwanti; Adra Amalia Nur Ahlina; Nadhifa Tazkia Ramadhani; Ratna Yuniati; Muhamad Sahlan; Kenny Lischer; Apriliana Cahya Khayrani; Amy Yayuk Sri Rahayu; Nidaan Khafian; Astari Dwiranti
Dharmakarya Vol 10, No 3 (2021): September, 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/dharmakarya.v10i3.32506

Abstract

Lebah merupakan hewan dari kelas Insecta yang menghasilkan produk kaya manfaat, diantaranya madu, propolis, dan bee pollen. Lebah, khususnya yang tanpa sengat, dapat dibudidayakan oleh masyarakat perkotaan karena relatif aman. Tim Pengabdian Masyarakat Multidisiplin Universitas Indonesia, yang terdiri dari Fakultas Matematika dan Ilmu Pengetahuan Alam, Fakultas Teknik, dan Fakultas Ilmu Administrasi bekerjasama dengan komunitas “3bee” telah melaksanakan serial workshop secara daring dengan topik pemanfaatan lebah. Selain itu, tim pengabdi dengan “holistika institute” juga mengajak masyarakat untuk memanfaatkan sampah rumah tangga agar dapat diolah kembali menjadi produk yang dapat menghidupkan perekonomian rumah tangga. Serial workshop terdiri dari empat seri, masing-masing terdiri dari dua sesi. Topik yang disampaikan meliputi pengenalan lebah dan urban bee; tanaman pakan lebah dan produk lebah; pangan sehat dari lebah; pengolahan sampah organik dan anorganik; serta pemasaran dan kualitas pelayanan. Workshop diselenggarakan selama 4 Pekan mulai tanggal 10 Oktober hingga 7 November 2020. Selain pemaparan narasumber, terdapat pre-test, post-test, dan evaluasi kegiatan dari peserta pada tiap seri. Peserta workshop berjumlah 171 orang yang berasal dari berbagai wilayah di Indonesia. Sebagian besar berasal dari kalangan akademisi, komunitas penggiat lebah, serta pegawai dinas lingkungan dan kehutanan. Berdasarkan hasil evaluasi, sebagian besar peserta merasa sangat puas (rentang kepuasan 4,21-5,00) dengan workshop yang terselenggara dan berencana mengimplementasikan pengetahuan yang diperoleh dari workshop ini (87%). Program pengabdian masyarakat multidisiplin ini dapat diduplikasi pada program lain sehingga penyampaian materi bersifat komprehensif. Program ini diharapkan dapat terus dikembangkan melalui program pendampingan sehingga lebah dan sampah dapat dimanfaatkan untuk meningkatkan ekonomi rumah tangga berkelanjutan di Indonesia.
STRUKTUR KOMUNITAS TUMBUHAN OBAT PASCAPERSALINAN DI PEKARANGAN MASYARAKAT SUNDA DESA CIBURIAL, BANTEN Siti Dian Rosadi; Nisyawati; Afiatry Putrika
Jurnal Pro-Life Vol. 7 No. 3 (2020): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Has conducted research on the structure of postpartum medicinal plants in the yard of the Sundanese community of Ciburial Village. Research developed to study what garden plants are used in postpartum medicine, as well as how they are in the yard. The research method used includes interviews and vegetation analysis. Interview results show that the plants used by the Sundanese community of Ciburial Village for the treatment of postpartum reached 46 species. Vegetation analysis results showed that as many as 36 species of post-delivery medicinal plants were obtained from the yard. Medicinal plants that have the highest Importance Value Index (INP) in the liana group are Areuy Hatta (Lygodium circinatum), terna group namely nampong (Siegesbeckia orientalis), shrub group namely harendong (Melastoma malabathricum), and these groups are called kalapa (Cocos nucifera ). Keywords: Banten, Ciburial, Yard, Medicine, Postpartum
INVENTARISASI TUMBUHAN KAWASAN SEMPADAN DI SITU AGATHIS, UNIVERSITAS INDONESIA, DEPOK, JAWA BARAT Shela Kartika Wijaya; Afiatry Putrika; Dimas Haryo Pradana; Sita Resmi
Al-Kauniyah: Jurnal Biologi Vol 10, No 1 (2017): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.049 KB) | DOI: 10.15408/kauniyah.v10i1.4517

Abstract

Abstrak Situ Agathis adalah salah satu dari enam situ di Universitas Indonesia, Depok. Situ Agathis merupakan situ pertama yang menerima aliran air dari pemukiman warga. Ekosistemnya meliputi badan air dan sempadan yang telah rusak akibat pembuangan sampah dan limbah rumah tangga di sekitar Kawasan Beji, Depok. Namun terdapat beberapa spesies tumbuhan yang dapat bertahan hidup di sekitarnya. Oleh karena itu, dilakukan inventarisasi untuk mengetahui jenis-jenis tumbuhan yang dapat bertahan dalam wilayah tercemar situ. Studi dilakukan menggunakan metode line transect dengan tiga kali pengulangan pada tiap stasiun inlet, midlet, dan outlet. Hasil studi menunjukkan adanya 59 spesies yang berasal dari 51 genus dan 30 famili. Famili dengan jumlah spesies terbanyak adalah Asteraceae, Fabaceae, dan Malvaceae. Habitus yang banyak ditemukan adalah herba (47,46%), semak (6,78%), liana (3,39%), perdu (5,08%), dan pohon (37,29%). Jenis yang banyak ditemui merupakan spesies invasif, gulma, tanaman perintis, dan tanaman bioremediator yang toleran terhadap lingkungan tercemar. Abstract Agathis lake is one of the six lakes around Universitas Indonesia, Depok. It is the first order lake that receives water flow from the settlements. Its ecosystem, which includes a body and border of water, has been damaged as a result of the disposal of garbage and household waste around Beji, Depok. However, there are several species of plants that can survive around Situ Agathis. Therefore, we performed an inventory to determine the types of plant which survive in the polluted region. The study was conducted by using line transect method with three repetitions at each station that were the inlet, midlet, and outlet. The result showed there were 59 species from 51 genera and 30 families in the border area.  Families with the highest number of species were Asteraceae, Fabaceae, and Malvaceae. The most commonly found habitus were herbs (47.46%), bushes (6.78%), liana (3.39%), shrubs (5.08%), and trees (37.29%). The widely encountered species were invasive species, weeds, pioneer plants, and bioremediator plants which tolerant to the polluted environment.
Analisis Morfologi Begonia isoptera Dryand. ex Sm (Begoniaceae) Kompleks di Jawa. Siti Azhyra Dwi Ayu; Afiatry Putrika; Deden Girmansyah
Floribunda Vol. 6 No. 2 (2019)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.056 KB) | DOI: 10.32556/floribunda.v6i2.2019.263

Abstract

Siti Azhyra Dwi Ayu, Afiatry Putrika & Deden Girmansyah. 2019. Analisis Morfologi Begonia isoptera Dryand. ex Sm (Begoniaceae) Kompleks di Jawa. Floribunda 6(2): 53–63. —  Begonia isoptera merupakan salah satu jenis Begonia di Jawa dengan konsep jenis yang luas, sehingga jenis ini menjadi kompleks. Pemeriksaan ulang morfologi jenis ini telah dilakukan terhadap semua koleksi B. isoptera di Herbarium Bogoriense dengan menggunakan 12 karakter vegetatif dan 7 karakter generatif terpilih. Hasil analisis menunjukkan bahwa B. isoptera terbagi menjadi 3 kelompok, yaitu: 1) berbulu (tangkai daun bertrikoma), 2) gundul (tangkai daun gundul), dan 3) kombinasi (campuran antara kelompok berbulu dan gundul). Analisis statistika menggunakan CA (Cluster Analysis) dan PCA (Principal Component Analysis) menunjukan bahwa B. isoptera kelompok berbulu dan B. isoptera kelompok kombinasi tergabung dalam satu kelompok besar, sedangkan B. isoptera kelompok gundul terpisah. Setelah dibandingkan dengan spesimen tipe dan pertelaan awal diketahui bahwa kelompok gundul merupakan B. isoptera yang sesuai dengan deskripsi awal, sedangkan kelompok berbulu sangat berbeda terutama pada sistem perbungaan dan indumentum tangkai daun sehingga akan diteliti lebih lanjut. Berdasarkan distribusi, B. isoptera kelompok berbulu dan kelompok kombinasi tumbuh di ketinggian 1000–2000 m dpl yang tersebar dari Jawa Barat sampai Jawa Tengah, sementara kelompok gundul tumbuh di dataran rendah Jawa Barat dan Banten pada ketinggian 0–600 m dpl. 
KEANEKARAGAMAN SPESIES LUMUT HATI EPIFIT DAN REKAMAN BARU UNTUK JAWA Afiatry Putrika; Shela Kartika Wijaya; Astari Dwiranti; Mega Atria
Floribunda Vol. 6 No. 4 (2020)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1622.535 KB) | DOI: 10.32556/floribunda.v6i4.2020.277

Abstract

Afiatry Putrika, Shela Kartika Wijaya, Astari Dwiranti, Mega Atria. 2020. Epiphytic Liverworts Diversity and New Record for Java. Floribunda 6(4): 133–140. —  Indonesia University (IU) has an open green space area, that consists of urban forest and the garden inside campus. The existence of these areas makes that location has various species of plants, especially tree species which become substrate for leafy liverworts. The objective of this study is to identify the leafy liverworts species diversity in the Campus of IU Depok. This research was conducted in the IU urban forest and campus main road by broad survey method. Those leafy liverworts sample was collected at 4 different direction of 0–2 m high of tree trunk. The liverworts samples were observed using a light microscope in the laboratory. There are 10 species of liverworts were recorded from Indonesia University which belong to 2 families and 6 genera. Two of them are new record for Java. Afiatry Putrika, Shela Kartika Wijaya, Astari Dwiranti, Mega Atria. 2020. Keanekaragaman Spesies Lumut Hati Epifit dan Rekaman Baru untuk Jawa. Floribunda 6(4): 133–140. —  Universitas Indonesia (UI) me-miliki kawasan ruang terbuka hijau yang terdiri atas hutan kota dan taman sekitar kampus. Keberadaan ruang terbuka tersebut membuat UI memiliki beragam jenis flora, termasuk pohon yang dapat menjadi substrat tumbuhnya lumut hati berdaun. Tujuan penelitian ini untuk mengetahui keanekaragaman spesies lumut hati epifit yang ada di Kampus UI Depok. Penelitian dilakukan di Hutan Kota dan jalan utama kampus UI Depok. Pengambilan sampel lumut hati di Hutan Kota dan jalan utama kampus dilakukan dengan metode jelajah bebas. Sampel lumut pada pohon inang dikoleksi dari 4 arah mata angin pada ketinggian 0–2 meter dari permukaan tanah. Sampel lumut diamati di laboratorium menggunakan mikroskop cahaya. Hasil penelitian menunjukkan terdapat 10 spesies lumut hati epifit di kampus UI yang berasal dari 2 famili dan 6 genus. Dua spesies merupakan catatan baru untuk Jawa. 
TREES PHYSIOLOGICAL RESPONSES TO AIR POLLUTION IN TAMAN MARGASATWA RAGUNAN AND UI DEPOK CAMPUS DIANA SELVIA HAMID; RATNA YULIATI; AFIATRY PUTRIKA
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 29 No. 3 (2022): BIOTROPIA Vol. 29 No. 3 Desember 2022
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2022.29.3.1764

Abstract

 Air pollution is a common environmental problem. Planting trees can minimize the adverse effects of air pollution. Plants can absorb and accumulate air pollutants through stomata. Biochemical changes in the leaves will appear as a physiological response of plants to air pollution that can be known by calculating the APTI (Air Pollution Tolerance Index) value. This study aimed to analyze the differences in physiological responses of five tree species in Taman Margasatwa Ragunan (TMR) South Jakarta and Universitas Indonesia (UI) Depok Campus as well as to find out the proper tree species planted in areas with high levels of air pollution. The leaves of five species (Hevea brasiliensis, Manilkara kauki, Artocarpus heterophyllus, Ficus septica, and Mangifera indica) were used to examine the effect of air pollution. Biochemical parameters (relative water content, leaf extract pH, total chlorophyll content, and ascorbic acid content) were observed from each species. The value of each parameter was calculated into the APTI equation. H. brasiliensis, F. septica, and M. indica were categorized as moderately tolerant plants, M. kauki were included as intermediate plants, and A. heterophyllus was a sensitive plant to air pollution in both locations. The highest APTI values were observed in M. indica in both locations. Thus, the recommended species planted in a polluted area was M. indica.
Karakteristik Lumut di Ruang Terbuka Hijau (RTH) di Area Permukiman Jakarta Selatan Sarah Tsabituddinillah; Afiatry Putrika; Niarsi Merry Hemelda; Andi Salamah; Windri Handayani; Astari Dwiranti; Mega Atria
Al-Kauniyah: Jurnal Biologi Vol 16, No 1 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i1.21811

Abstract

AbstrakPermukiman merupakan salah satu ruang terbuka hijau (RTH) yang terdapat di daerah urban, khususnya Jakarta. Salah satu kelompok tumbuhan yang ditemui pada RTH tersebut adalah lumut. Keberadaan lumut di permukiman urban menunjukkan adanya kemampuan lumut untuk bertahan pada lingkungan yang terganggu. Penelitian ini bertujuan untuk mengetahui spesies lumut serta karakteristik lumut di salah satu permukiman Jakarta Selatan. Lumut dikoleksi dengan metode transect-line pada 6 titik tepi jalan dan jelajah bebas pada 3 taman di permukiman tersebut Jakarta Selatan. Pengamatan karakteristik morfologi dan anatomi lumut dilakukan dengan penilaian kualitatif dan kuantitatif. Berdasarkan hasil penelitian terdapat 2 divisi lumut, yaitu Bryophyta (lumut sejati) dan Marchantiophyta (lumut hati) di lokasi penelitian. Bryophyta terdiri dari 6 famili, 9 genus, dan 16 spesies. Sementara itu,  Marchantiophyta terdiri dari 2 famili, 2 genus, dan 3 spesies. Pottiaceae merupakan famili dengan jumlah spesies terbanyak ditemukan, yaitu 5 spesies. Fissidens biformis adalah spesies dengan jumlah sampel terbanyak. Lumut tersebut ditemukan pada substrat tanah, batu, dan batang pohon. Kisaran luas tutupan lumut yang ditemukan yaitu 2–100%. Karakteristik seperti ukuran tubuh yang kecil, bentuk hidup, bentuk daun, ornamentasi pada permukaan daun, modifikasi sel daun, serta keberadaan sporofit atau gemma diduga mendukung lumut beradaptasi di lingkungan urban.AbstractSettlement is one of urban green open spaces in Jakarta. One of the plant groups found in the open green spaces is the bryophytes. The presence of bryophytes in the settlement areas indicates the ability of bryophytes to survive in a disturbed environment. This study aims to determine bryophytes species and their characteristic in the settlements area of South Jakarta. Bryophyte collected by transect-line at 6 sites of roadside and broad survey at 3 sites of park. The morphological and anatomical characteristics were observed with qualitative and quantitative assessments. Mosses and liverworts are groups that found in study sites. The mosses consists of 6 families, 9 genera, and 16 species. Meanwhile, the liverworts consists of 2 families, 2 genera, and 3 species. Pottiaceae is has the highest species richness in the location. Meanwhile the highest number of samples was Fissidens biformis. The bryophytes were attached in the soil, rock, and tree trunk. The coverage of bryophyte is about 2–100%. Characteristics such as small body size, life-forms, leaf shape, the ornamentation on the leaf surface, modified leaf cells, and the presence of sporophyte or gemmae are thought to support the adaptation of bryophyte in urban environments.
Six tree species physiological responses to air pollution in Pulogadung Industrial Estate, East Jakarta, Indonesia and Universitas Indonesia Campus, Depok, Indonesia Ananda Putri; Ratna Yuniati; Afiatry Putrika
International Journal of Advances in Applied Sciences Vol 12, No 2: June 2023
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijaas.v12.i2.pp152-162

Abstract

Air pollution is a global issue that has a harmful impact on living things and the environment. It is commonly recognized that bioremediation, including the use of tree plants, helps reduce air pollution. Tree plants can respond physically to air pollution. The value of the air pollution tolerance index (APTI) can be used to determine the physiological response. Based on APTI values, this study seeks to determine the tolerance levels and physiological response differences of six tree plant species (Mangifera indica, Pterocarpus indicus, Cerbera odollam, Pometia pinnata, Syzygium myrtifolium, and Swietenia macrophylla) in Pulogadung Industrial Estate, East Jakarta and Universitas Indonesia (UI) Campus, Depok. Environmental factors and APTI values with relative water content parameters, leaf extract pH, ascorbic acid content, and total chlorophyll content were measured in six kinds of tree plants at both research sites. The maximum APTI score in the Pulogadung Industrial Estate was 9.79 0.13, indicating that Mangifera indica plants are air pollution tolerant. Meanwhile, Pterocarpus indicus is classified as sensitive to air pollution, with the lowest APTI score of 6.59 0.18 at the UI Campus, Depok. The APTI test results revealed that tolerant species had high relative water content (RWC) values and ascorbic acid concentration, whereas sensitive species had low RWC values and poor total chlorophyll content.