Kanicnan Intui
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Estimation of Chronological Age from Advanced Glycation End Products Level in Vitreous Fluid Kanicnan Intui; Churdsak Jaikang; Somlada Watcharakhom; Yutti Amornlertwatana
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14301

Abstract

Background: Chronological age estimation is an important process for corpse identification. Advancedglycation end products (AGEs) are accumulated during lifetime and have been used for forensic identification.The AGEs in vitreous humor still lack of information for age estimation. Objective: To investigate correlationbetween the AGEs level contained in vitreous humor and chronological age in postmortem cases. Methods:The vitreous humor samples were collected from the postmortem cases (n=142). The AGEs levels weredetermined by spectrofluorometer and presented in microgram of quinidine hemisulfate equivalent (?g QE/mL) and nanogram of pentosidine equivalent (ng PE/mL). Stepwise linear regression was used to generatethe equation. Results: The QE and PE showed mild positive correlation (r = 0.350 and 0.195; p <0.001,respectively) while the PE/QE ratio showed moderate negative correlation with age (r = -0.561, p <0.001).The predicted equation showed error value 12 years and gave accurate prediction in range 40-59 years old.Conclusion: The AGEs contained in the vitreous humor correlated with chronological age in postmortemcases. The other fluorescence molecules might be interfere during the AGEs measurement then the specificmethods should be developed for measuring the AGEs level to decrease the error value.
Gender Differentiation in Tryptophan Metabolism in Postmortem Cases Somlada Watcharakhom; Kanicnan Intui; Yutti Amornlertwatana; Churdsak Jaikang
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15955

Abstract

Tryptophan (TRP) is an essential amino acid and is metabolized via kynurenine and serotonin pathway. Both pathways are significant role in many diseases and have been studied as biomarkers for differentiate and clarify in many diseases including forensic works. Metabolism in the body might be affected by both intrinsic and extrinsic factors. This study aimed to compare postmortem blood level of TRP and its metabolites in male and female to be applied in gender identification in forensic medicine cases. Blood samples were collected from male group (n=11) and female group (n=11) and the level of the TRP and its metabolites were identified by HPLC-DAD. The values of the TRP, metabolites and metabolite ratios did not different between the groups. While, the value of ratio of picolinic acid/ quinolinic acid different significantly (p <0.05) among the groups which might be applied for gender identification in postmortem cases. In conclusion, we found that gender have no effect on the TRP and its metabolites. By the way, the effect of gender should be expanded sample size and develop to gender identification method.