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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Hemera Zoa
. Hidayati
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Single Nucleotide Polymorphisms (SNPs) in Exon 6 of Lecithin Cholesterol Acyltransferase (LCAT) Gene in Indonesian Local Sheep . Hidayati; Cece Sumantri; RR Noor; R Priyanto
Media Peternakan Vol. 37 No. 2 (2014): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1040.268 KB) | DOI: 10.5398/medpet.2014.37.2.71

Abstract

Lecithin cholesterol acyltransferase (LCAT) is a soluble enzyme that converts cholesterol and lecithin to cholesteryl esters and lysolecithins on the surface of high density lipoprotein and plays an important role in lipoprotein metabolism. The research was aimed to explore single nucleotide polymorphisms of LCAT gene in Indonesian local sheep. A total of 118 genomic DNA of Indonesian local sheep were used in this research, consisted of Sumatera Thin Tail (43 heads), Garut (19 heads), Javanese Thin Tail (17 heads), Javanese Fat Tail (6 heads), Rote Island (7 heads), Kissar (7 heads), Sumbawa (10 heads), and Lembah Palu (9 heads). Polymerase chain reaction was used to amplify genomic DNA for exon 6 (250 bp) and direct sequencing method was used to identify polymorphism sequences. The sequences were analyzed with BioEdit and MEGA 5.2 software. The BLAST sequence was obtained from Gene Bank GQ 150556.1. The results showed three novel SNPs, i.e. c.742C>T, c.770 T>A and c.882C>T. Substitution of cytosine to thymine c.742 is a synonymous mutation; thymine to adenine c.770 and cytosine to thymine c.882 are non-synonymous mutations. Polymorphisms of LCAT gene exon 6 was found in Sumatera Thin Tail, Javanese Thin Tail, Javanese Fat Tail, Garut, Lembah Palu, and Rote Island.
PF-13 Fracture Fixation between Os Lumbalis II and III on a Three Month Old Kitten Using Parallel Technique Siti Zaenab; Ivan Satriawan; Osye S Alamsari; Eva Zulfiati; Benda Alifianti; . Hidayati; Deni Noviana
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (482.274 KB)

Abstract

Spinal fractures, luxations, and fracture- luxations in dogs and cats are common and are often the result of vehicular injury. Other frequent causes of injury include animal attacks or falling from a height. Reports that describe the distribution of spinal column injuries indicate that they affect the lumbar vertebrae most frequently, followed by sacrococcygeal, thoracic, and cervical vertebrae2,3.
Co-Authors Benda Alifianti Cece Sumantri Deni Noviana Eva Zulfiati Ivan Satriawan Osye S Alamsari R Priyanto RR Noor Siti Zaenab