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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology agriTECH JHeS (Journal of Health Studies)
Muslih Anwar
BPTBA - LIPI Gunung Kidul
Agriculture, Biological Sciences & Forestry Humanities Education Health Professions Nursing Public Health

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Isolation of Lactic Acid Bacteria with Cholesterol-Lowering Activity from Digestive Tracts of Indonesian Native Chickens H. Julendra; Ade Erma Suryani; Lusty Istiqomah; Ema Damayanti; Muslih Anwar; Naditya Fitriani
Media Peternakan Vol. 40 No. 1 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (543.536 KB) | DOI: 10.5398/medpet.2017.40.1.35

Abstract

The aim of the study was to identify the cholesterol-lowering activity of indigenous lactic acid bacteria isolated from the small intestine, cecum, and colon of Indonesian native chickens and evaluated for bile salt hydrolase (BSH) activity in vitro by using MRS media added taurodeoxycholic acid (TDCA) and CaCl2. The quantitative measurement of cholesterol-lowering activity of LAB was investigated by using soluble cholesterol containing MRS broth (100 µg/mL of cholesterin) and incubated at 37 °C for 48 h. Cholesterol content in supernatant was analyzed using microplate reader. The highest percentage of cholesterol reduction found in isolates from colon of native chicken with the value of 17.43% and identified as Lactobacillus plantarum. Based on phylogenetic tree analysis, this isolate was closely related to L. plantarum strain LGFCP4 (accession number KM199683.1) isolated from GIT of Guinea fowl from India. It could be concluded that L. plantarum AKK-30 had cholesterol-lowering activity.
Viabilitas Dan Aktivitas Antibakteri Bifidobacterium bifidum Dalam Susu Bifidus Dengan Suplementasi Ekstrak Cengkeh (Syzygium aromaticum) Nosa Septiana Anindita; Muslih Anwar
agriTECH Vol 41, No 3 (2021)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (52.641 KB) | DOI: 10.22146/agritech.40882

Abstract

Pengembangan produk olahan susu kambing terfermentasi dan diperkaya dengan probiotik Bifidobacterium bifidum merupakan salah satu produk pangan fungsional (Functional Food) yang selanjutnya disebut sebagai Susu Bifidus. Susu bifidus sebagai salah satu produk fermentasi susu dapat dikonsumsi oleh masyarakat yang mengalami kesulitan mencerna laktosa (lactose intolerance). Daya terima konsumen terhadap susu kambing masih tergolong rendah dibandingkan dengan susu sapi, sehingga hal tersebut juga perlu diantisipasi. Penambahan ekstrak cengkeh (Syzygium aromaticum) pada susu Bifidus dapat digunakan sebagai alternatif senyawa aromatik alami, untuk menghilangkan aroma goaty pada susu kambing yang kurang begitu disukai oleh konsumen. Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi ekstrak cengkeh terhadap viabilitas (daya hidup) dan aktivitas antibakteri Bifidobacterium bifidum serta kualitas kimia susu Bifidus. Parameter yang diamati pada penelitian adalah total Bifidobacterium bifidum, total bakteri dan luas zona bening susu Bifidus. Suplementasi ekstrak cengkeh sebesar 2% sebagai bahan suplementasi pada Bifidus milk dapat meningkatkan viabilitas probiotik Bifidobacterium bifidum. Ekstrak cengkeh dalam produk Bifidus milk berperan sebagai agen preservatif, didukung dengan adanya aktivitas antibakteri terhadap Staphylococcus aureus dan Salmonella typhimurium dan penurunan total bakteri pada Bifidus milk.
Kemampuan Metabolisme Asam Linoleat oleh Kandidat Probiotik Potensial Asal Feses Bayi Asli Indonesia Nosa Septiana Anindita; Muslih Anwar Widodo; Tiyas Tono Taufik; Tutik Dwi Wahyuningsih
JHeS (Journal of Health Studies) Vol 2, No 1: Maret 2018
Publisher : Universitas 'Aisyiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (539.43 KB) | DOI: 10.31101/jhes.433

Abstract

Six probiotic candidates were grown overnight at 37 °C in De Man Rogosa Sharpe(MRS) broth supplemented with 0.4 mg/mL of linoleic acid. The concentrations of the metabolite products in the culture media were determined using GC-MS after extraction and direct transesterification of the fatty acids. Linoleic acid was added to the sample as an internal standard. The result showed that Lactobacilluscasei strainAPwas able to convertfreelinoleicacidto conjugated linoleic acid in themedia more than 60% while five other potential probiotics such as Lactobacillus casei AF, Lactobacillus casei AG, Pediococcus acidilactici AA, Pediococcus acidilactici BE and Pediococcus acidilactici BK have abilities to convert free linoleic acid to palmitic acid, stearic acid and oleic acid with varying percentages.
Co-Authors Ade Erma Suryani Ema Damayanti H. Julendra Lusty Istiqomah Naditya Fitriani Nosa Septiana Anindita Tiyas Tono Taufik Tutik Dwi Wahyuningsih