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Identification of Pediococcus Strains Isolated from Feces of Indonesian Infants With in vitro Capability to Consume Prebiotic Inulin and to Adhere on Mucus ., Widodo; Anindita, Nosa Septiana; Taufiq, Tiyas Tono; Wahyuningsih, Tutik Dwi
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.164 KB)

The aim of this experiment was to identify isolates obtained from feces of Indonesian infants and to evaluate their capability as probiotics. Identification of isolates was carried out based on morphology, physiology and biochemical identifications, and molecular identification based on 16S rRNA sequence. Morphological and physiological identification was carried out based on Gram staining, shape, motility, spore formation and catalase production. Biochemical identifications based on production of CO2 and NH3 from glucose, the ability to grow on different temperature (10 and 45°C) and pH (4.4 and 9.6), and different salt concentration (6.5 and 18%). Probiotics capability of isolates was assayed on the ability to grow on low pH (pH 2.0), on different bile salts concentration (0.3; 0.5; 1.0 and 1.5%), the capacity to grow on media with inulin as the only carbon source, and in vitro adhesion ability on porcine mucin. Morphological, physiological and biochemical identification suggest that all of isolates belong to lactic acid bacteria. Further molecular identification of five isolates showedthat isolates AA, BE and BK were strains of Pediococcus acidilactici (similarity 99%), while isolate AP and AG were strains of Lactobacillus casei (similarity 99-100%). Probiotic assays showed that more than 80% of cells of Pediococcus acidilactici isolates AA, BE and BK were viable after grown on pH 2.0 for 90 min, and around 80% of cells from the same isolates were survived on media supplemented with bile salt 1.5% for 2 h. All of isolates had high adhesion capacity as seen by more than 75% of cells attached on pig gastric mucin. Investigationof isolates to grow on inulin showed Pediococcus acidilactici isolate BE was able to consume inulin as the only carbon source. It is concluded that Pediococcus acidilactici isolate BE was a candidate probiotics and subject to further in vivo evaluation using animal models to examine their beneficial health effects.Key word : Pediococcus acidilactici, Lactobacillus casei, human origin and probiotics.

AKTIVITAS ANTIBAKTERI Lactobacillus paracasei ASAL AIR SUSU IBU (ASI) TERHADAP BAKTERI PATOGEN Anindita, Nosa Septiana
Biomedika Vol 13, No 1 (2021): Biomedika Februari 2021
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v13i1.10830

ABSTRAKAktivitas antibakteri merupakan salah satu kriteria bagi isolat kandidat probiotik untuk dapat diaplikasikan dalam pangan fungsional. Pemanfaatan Air Susu Ibu (ASI) sebagai sumber probiotik didasari bahwa ASI merupakan bahan pangan kaya nutrisi yang mengandung human milk oligosaccharide (HMOs) sehingga menghasilkan efek bifidogenik berupa pertumbuhan bakteri menguntungkan, diantaranya adalah kelompok Bakteri Asam Laktat (BAL). Keunggulan BAL asal ASI yaitu memiliki potensi sebagai probiotik sehingga akan diperoleh isolat lokal probiotik terseleksi. Probiotik yang berasal dari manusia seperti ASI, berpeluang besar memiliki viabilitas tinggi dan adaptif pada saluran pencernaan ketika dimanfaatkan sebagai pangan fungsional. Penelitian ini diharapkan memperoleh kultur lokal probiotik asal ASI yang potensial dalam melawan bakteri patogen. Parameter pengujian yang diamati adalah aktivitas antibakteri yang dimiliki oleh 3 strain Lactobacillus paracasei (L. paracasei) asal ASI yaitu L. paracasei strain AS9, L. paracasei strain AS10 dan L. paracasei strain AS12. Sebanyak 4 bakteri patogen digunakan dalam pengujian ini, meliputi Enterococcus faecalis (Entr. faecalis) 99 EF, Staphylococcus aureus (Stph. aureus) FNCC 0047, Escherichia coli (E. coli) FNCC 0091 dan Shigella flexneri (S. flexnerii) ATCC 12022. Aktivitas penghambatan diperoleh dengan mengukur luasan zona bening yang dihasilkan selama proses inkubasi pada suhu 37ÂºC, 48 jam dengan metode sumuran. Berdasarkan hasil pengujian, menunjukkan bahwa Lactobacillus paracasei strain AS12 memiliki aktivitas penghambatan tertinggi dengan golongan daya hambat kuat berdasarkan luasan zona bening terhadap 4 bakteri pathogen uji. Sehingga Lactobacillus paracasei strain AS12 berpotensi dalam penghambatan bakteri patogen. Â Kata Kunci: Bakteri Asam Laktat (BAL), Air Susu Ibu (ASI), L. paracasei, AntibakteriÂ ABSTRACTÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Antibacterial activity is one of the criteria for probiotic candidate isolates to be applied in functional foods. The utilization of human milk as a probiotics source is due to nutrient-rich food containing human milk oligosaccharides (HMOs) to produce a bifidogenic factor growth of beneficial bacteria, including Lactic Acid Bacteria (LAB). The beneficial role LAB isolated from human milk is that it has the potential as a source of local probiotic isolates. Probiotics derived from humans, such as breast milk, are likely to have high viability and adaptability to the digestive tract when used as a functional food. This research is observed local cultures of probiotics from breast milk that have the potential against pathogenic bacteria. The parameters observed were the antibacterial activity of three strains of Lactobacillus paracasei (L. paracasei) asal ASI yaitu L. paracasei strain AS9, L. paracasei strain AS10 and L. paracasei strain AS12. A total of four pathogenic bacterias were used, including Enterococcus faecalis (Entr. faecalis) 99 EF, Staphylococcus aureus (Stph. aureus) FNCC 0047, Escherichia coli (E. coli) FNCC 0091 and Shigella flexneri (S. flexnerii) ATCC 12022Inhibition zone was obtained by measuring the clear zone area produced during incubation at 37ÂºC for 48 h with the well diffusion method. The results showed that Lactobacillus paracasei strain AS12 had the highest inhibitory activity with the strong inhibition category based on the clear zone area against the four pathogenic bacterias. So that L. paracasei strain AS12 has the potential to inhibit pathogenic bacteria.Â Keywords: Lactic Acid Bacteria (LAB), Human milk, L. paracasei, antibacterial

KAJIAN MOLEKULER PADA PROBIOTIK ASAL AIR SUSU IBU DALAM SINTESIS EKSOPOLISAKARIDA (EPS) Hamiyawati Qoimatu Dini Alfaruqi; Nosa Septiana Anindita; Arif Bimantara
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Molecular Studies on Probiotic of Human Breast Milk in the Synthesis of Exopolysaccharide (EPS) The glucosyltransferase (gtf) gene has an important role in exopolysaccharide (EPS) synthesis in probiotic bacteria. The EPS produced is associated with the adhesion ability of bacteria to the intestinal mucosa. Therefore, the gtf gene can be used as a parameter in the selection of potential probiotic through a molecular approach. This study was conducted to determine the presence of the gtf gene in probiotic from human breast milk using PCR technique. The methods in this study include the following: reculture of probiotic isolates, DNA isolation, amplification of the 16S rRNA gene using universal primers (pA and pB), amplification of specific LAB primers (LABfw and LABrv), specific primary design for the gtf gene, and the amplification of the gtf gene. The results of 16S rRNA gene amplification using universal primers obtained the amplicons of 500-1,000 bp in size. The results of amplification using specific LAB primers obtained an amplicon of about 700 bp in all isolates. The results of amplification of the gtf gene using a specific primer produced an amplicon of 325 bp in all isolates. Based on this study, it was concluded that 16 probiotic isolates from human breast milk were proven to have the gtf gene. Gen glukosiltransferase (gtf) memiliki peran penting dalam sintesis eksopolisakarida (EPS) pada bakteri probiotik. EPS yang diproduksi berhubungan dengan kemampuan adhesi bakteri pada mukosa usus. Oleh karena itu, gen gtf dapat dijadikan sebagai salah satu parameter dalam seleksi probiotik potensial melalui pendekatan molekuler. Penelitian ini dilakukan untuk mengetahui adanya gen gtf pada probiotik asal air susu ibu (ASI) menggunakan teknik PCR. Metode pada penelitian ini meliputi: reculture isolat probiotik, isolasi DNA, amplifikasi gen 16S rRNA menggunakan primer universal (pA dan pB), amplifikasi primer spesifik BAL (LABfw dan LABrv), desain primer spesifik untuk gen gtf dan amplifikasi gen gtf. Hasil amplifikasi gen 16S rRNA menggunakan primer universal diperoleh amplikon berukuran antara 500-1.000 bp. Adapun hasil amplifikasi menggunakan primer spesifik BAL diperoleh amplikon berukuran sekitar 700 bp pada seluruh isolat. Hasil amplifikasi gen gtf menggunakan primer spesifik menghasilkan amplikon berukuran sekitar 325 bp pada seluruh isolat. Berdasarkan penelitian ini dapat disimpulkan bahwa 16 isolat probiotik asal ASI terbukti memiliki gen gtf.

Viabilitas Dan Aktivitas Antibakteri Bifidobacterium bifidum Dalam Susu Bifidus Dengan Suplementasi Ekstrak Cengkeh (Syzygium aromaticum) Nosa Septiana Anindita; Muslih Anwar
agriTECH Vol 41, No 3 (2021)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Pengembangan produk olahan susu kambing terfermentasi dan diperkaya dengan probiotik Bifidobacterium bifidum merupakan salah satu produk pangan fungsional (Functional Food) yang selanjutnya disebut sebagai Susu Bifidus. Susu bifidus sebagai salah satu produk fermentasi susu dapat dikonsumsi oleh masyarakat yang mengalami kesulitan mencerna laktosa (lactose intolerance). Daya terima konsumen terhadap susu kambing masih tergolong rendah dibandingkan dengan susu sapi, sehingga hal tersebut juga perlu diantisipasi. Penambahan ekstrak cengkeh (Syzygium aromaticum) pada susu Bifidus dapat digunakan sebagai alternatif senyawa aromatik alami, untuk menghilangkan aroma goaty pada susu kambing yang kurang begitu disukai oleh konsumen. Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi ekstrak cengkeh terhadap viabilitas (daya hidup) dan aktivitas antibakteri Bifidobacterium bifidum serta kualitas kimia susu Bifidus. Parameter yang diamati pada penelitian adalah total Bifidobacterium bifidum, total bakteri dan luas zona bening susu Bifidus. Suplementasi ekstrak cengkeh sebesar 2% sebagai bahan suplementasi pada Bifidus milk dapat meningkatkan viabilitas probiotik Bifidobacterium bifidum. Ekstrak cengkeh dalam produk Bifidus milk berperan sebagai agen preservatif, didukung dengan adanya aktivitas antibakteri terhadap Staphylococcus aureus dan Salmonella typhimurium dan penurunan total bakteri pada Bifidus milk.

Identification of Pediococcus Strains Isolated from Feces of Indonesian Infants With in vitro Capability to Consume Prebiotic Inulin and to Adhere on Mucus W. Widodo; Nosa Septiana Anindita; Tiyas Tono Taufiq; Tutik Dwi Wahyuningsih
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

The aim of this experiment was to identify isolates obtained from feces of Indonesian infants and to evaluate their capability as probiotics. Identification of isolates was carried out based on morphology, physiology and biochemical identifications, and molecular identification based on 16S rRNA sequence. Morphological and physiological identification was carried out based on Gram staining, shape, motility, spore formation and catalase production. Biochemical identifications based on production of CO2 and NH3 from glucose, the ability to grow on different temperature (10 and 45°C) and pH (4.4 and 9.6), and different salt concentration (6.5 and 18%). Probiotics capability of isolates was assayed on the ability to grow on low pH (pH 2.0), on different bile salts concentration (0.3; 0.5; 1.0 and 1.5%), the capacity to grow on media with inulin as the only carbon source, and in vitro adhesion ability on porcine mucin. Morphological, physiological and biochemical identification suggest that all of isolates belong to lactic acid bacteria. Further molecular identification of five isolates showedthat isolates AA, BE and BK were strains of Pediococcus acidilactici (similarity 99%), while isolate AP and AG were strains of Lactobacillus casei (similarity 99-100%). Probiotic assays showed that more than 80% of cells of Pediococcus acidilactici isolates AA, BE and BK were viable after grown on pH 2.0 for 90 min, and around 80% of cells from the same isolates were survived on media supplemented with bile salt 1.5% for 2 h. All of isolates had high adhesion capacity as seen by more than 75% of cells attached on pig gastric mucin. Investigationof isolates to grow on inulin showed Pediococcus acidilactici isolate BE was able to consume inulin as the only carbon source. It is concluded that Pediococcus acidilactici isolate BE was a candidate probiotics and subject to further in vivo evaluation using animal models to examine their beneficial health effects.Key word : Pediococcus acidilactici, Lactobacillus casei, human origin and probiotics.

AKTIVITAS ANTIBAKTERI Lactobacillus paracasei ASAL AIR SUSU IBU (ASI) TERHADAP BAKTERI PATOGEN Nosa Septiana Anindita
Biomedika Vol 13, No 1 (2021): Biomedika Februari 2021
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v13i1.10830

ABSTRAKAktivitas antibakteri merupakan salah satu kriteria bagi isolat kandidat probiotik untuk dapat diaplikasikan dalam pangan fungsional. Pemanfaatan Air Susu Ibu (ASI) sebagai sumber probiotik didasari bahwa ASI merupakan bahan pangan kaya nutrisi yang mengandung human milk oligosaccharide (HMOs) sehingga menghasilkan efek bifidogenik berupa pertumbuhan bakteri menguntungkan, diantaranya adalah kelompok Bakteri Asam Laktat (BAL). Keunggulan BAL asal ASI yaitu memiliki potensi sebagai probiotik sehingga akan diperoleh isolat lokal probiotik terseleksi. Probiotik yang berasal dari manusia seperti ASI, berpeluang besar memiliki viabilitas tinggi dan adaptif pada saluran pencernaan ketika dimanfaatkan sebagai pangan fungsional. Penelitian ini diharapkan memperoleh kultur lokal probiotik asal ASI yang potensial dalam melawan bakteri patogen. Parameter pengujian yang diamati adalah aktivitas antibakteri yang dimiliki oleh 3 strain Lactobacillus paracasei (L. paracasei) asal ASI yaitu L. paracasei strain AS9, L. paracasei strain AS10 dan L. paracasei strain AS12. Sebanyak 4 bakteri patogen digunakan dalam pengujian ini, meliputi Enterococcus faecalis (Entr. faecalis) 99 EF, Staphylococcus aureus (Stph. aureus) FNCC 0047, Escherichia coli (E. coli) FNCC 0091 dan Shigella flexneri (S. flexnerii) ATCC 12022. Aktivitas penghambatan diperoleh dengan mengukur luasan zona bening yang dihasilkan selama proses inkubasi pada suhu 37ºC, 48 jam dengan metode sumuran. Berdasarkan hasil pengujian, menunjukkan bahwa Lactobacillus paracasei strain AS12 memiliki aktivitas penghambatan tertinggi dengan golongan daya hambat kuat berdasarkan luasan zona bening terhadap 4 bakteri pathogen uji. Sehingga Lactobacillus paracasei strain AS12 berpotensi dalam penghambatan bakteri patogen. Kata Kunci: Bakteri Asam Laktat (BAL), Air Susu Ibu (ASI), L. paracasei, Antibakteri ABSTRACT Antibacterial activity is one of the criteria for probiotic candidate isolates to be applied in functional foods. The utilization of human milk as a probiotics source is due to nutrient-rich food containing human milk oligosaccharides (HMOs) to produce a bifidogenic factor growth of beneficial bacteria, including Lactic Acid Bacteria (LAB). The beneficial role LAB isolated from human milk is that it has the potential as a source of local probiotic isolates. Probiotics derived from humans, such as breast milk, are likely to have high viability and adaptability to the digestive tract when used as a functional food. This research is observed local cultures of probiotics from breast milk that have the potential against pathogenic bacteria. The parameters observed were the antibacterial activity of three strains of Lactobacillus paracasei (L. paracasei) asal ASI yaitu L. paracasei strain AS9, L. paracasei strain AS10 and L. paracasei strain AS12. A total of four pathogenic bacterias were used, including Enterococcus faecalis (Entr. faecalis) 99 EF, Staphylococcus aureus (Stph. aureus) FNCC 0047, Escherichia coli (E. coli) FNCC 0091 and Shigella flexneri (S. flexnerii) ATCC 12022Inhibition zone was obtained by measuring the clear zone area produced during incubation at 37ºC for 48 h with the well diffusion method. The results showed that Lactobacillus paracasei strain AS12 had the highest inhibitory activity with the strong inhibition category based on the clear zone area against the four pathogenic bacterias. So that L. paracasei strain AS12 has the potential to inhibit pathogenic bacteria. Keywords: Lactic Acid Bacteria (LAB), Human milk, L. paracasei, antibacterial

Identifikasi Bakteri Patogen Pada Olahan Pangan Saat Arus Mudik Lebaran Di Terminal Tirtonadi Surakarta Afifah Nurul Falih; Nosa Septiana Anindita; Emmanuel Kristanti
Jurnal Kesehatan Vol 14, No.1 (2021): Jurnal Kesehatan
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/jk.v14i1.11056

Keamanan pangan pada olahan pangan yang diperjual belikan menjadi perhatian bagi masyarakat terutama akibat yang ditimbulkan. Oleh karena itu, perlu dilakukan tindakan pengawasan melalui identifikasi terkait keberadaan bakteri patogen pada olahan pangan. Tujuan dari penelitian ini adalah mengidentifikasi keberadaan bakteri patogen dalam olahan pangan selama mudik lebaran di Terminal Tirtonadi Surakarta. Metode identifikasi yang digunakan meliputi pengambilan dan pengkayaan sampel, uji konfirmasi, uji penegasan dan analisis data. Hasil yang didapatkan dari penelitian ini yaitu dari 11 sampel olahan pangan, semua sampel hasil yang diperoleh negatif (-) terhadap bakteri Salmonella sp, Vibrio cholera (V.cholera), Staphylococcus aureus (S. aureus), Bacillus cereus (B. Cereus) akan tetapi 6 sampel teridentifikasi positif (+) cemaran Eschericia coli (E.coli). Sehingga, dapat disimpulkan bahwa dari beberapa jenis bakteri patogen sebagai indikator cemaran olahan bahan pangan, terdapat satu cemaran bakteri patogen yang positif ditemukan dalam 6 sampel olahan pangan yaitu E.coli.Kata kunci : Bakteri patogen, Escherichia coli, keamanan pangan.

GERAKAN MEMASYARAKATKAN MAKAN IKAN SEJAK USIA DINI MELALUI PELATIHAN PENGOLAHAN IKAN BAGI ORANG TUA SISWA KELAS I SD MUHAMMADIYAH WIROBRAJAN 3 YOGYAKARTA Arif Bimantara; Nosa Septiana Anindita
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol 2, No 2 (2018): Vol 2, No 2 (2018)
Publisher : Yogyakarta State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.777 KB)

Gerakan memasyarakatkan makan ikan (GEMARIKAN) adalah salah satu upaya pemerintah dalam meningkatkan tingkat konsumsi ikan masyarakat Indonesia. Ikan merupakan salah satu sumber nutrien penting bagi manusia khususnya bagi anak-anak. SD Muhammadiyah Wirobrajan 3 Yogyakarta merupakan salah satu sekolah dasar berprestasi di Kota Yogyakarta yang mengedepankan tidak hanya prestasi tetapi juga kesehatan anak didiknya. Akan tetapi, sosialisasi mengenai pentingnya konsumsi ikan bagi anak-anak kepada orang tua murid masih belum dilakukan. Pengabdian ini bertujuan untuk mengenalkan GEMARIKAN kepada orang tua murid beserta pelatihan pengolahan daging ikan menjadi kreasi makanan yang menarik bagi anak-anak.Tahapan pengabdian dimulai dari persiapan meliputi perizinan, persiapan bahan baku dan penyusunan buku panduan. Selanjutnya dilakukan penyuluhan dan pelatihan pengolahan ikan terhadap 20 orang perwakilan orang tua murid kelas 1. Evaluasi dilakukan setelah rangkaian acara pengabdian selesai dengan mengadakan lomba kreasi masakan berbahan dasar daging ikan. Hasil pengabdian menunjukkan bahwa orang tua siswa antusias dengan pengabdian yang dilakukan ditunjukkan dengan peran aktif mereka dalam kegiatan. Selanjutnya pengabdian bisa dilakukan dengan lebih banyak peserta agar manfaat yang diberikan menjadi lebih luas dan mengikutsertakan ahli masak kantin sekolah sebagai perserta.

Isolasi Dan Identifikasi Fenotipik Bakteri Asam Laktat (BAL) Indigenous Asal Air Susu Ibu (ASI) Nosa Septiana Anindita
Jurnal Teknologi Pangan Vol 5, No 1 (2021)
Publisher : Program Studi Teknologi Pangan

Currently the use of probiotic food is growing rapidly, especially in Indonesia along with the development of fermentation technology. Most of the probiotic isolates come from abroad which has an impact on the high price of probiotic food products. Mother's milk is one source of lactic acid bacteria (LAB), which serves to maintain the balance of the digestive tract microflora and enhance the immune system. Lactic acid bacteria as probiotics have the chance to live in the digestive tract if they come from the human body. Based on this matter, it is necessary to conduct research as an effort to obtain indigenous BAL isolates from ASI. This study aims to obtain local BAL isolates from ASI through phenotypic identification. Phenotypic identification includes the morphology of colonies and cells, Gram, catalase test, motility and spore formation and biochemical identification including test of the ability to grow at various growth temperatures, low pH, salt content and formation of CO2 from glucose. Based on biochemical identification results, out of 38 BAL isolates that were successfully identified biochemically consisted of several genera of BAL namely Lactobacillus (9 isolates), Pediococcus (3 isolates) and Weisella (20 isolates).

The Detection of Genes Encoding Enzyme Fructosyltransferase The Exopolysaccharide (EPS) Produce Potential Probiotic Candidates For Breast Milk (ASI) Afifah Nurul Falih; Nosa Septiana Anindita; Arif Bimantara; Annisa Khumaira
International Journal of Health Science and Technology Vol 4, No 1 (2022): July
Publisher : Universitas 'Aisyiyah Yogyakarta

Bacteria is one of the microorganisms that we can easily find in nature. Bacteria can be classified into benefical bacteria and harmful bacteria. One of the bacteria classified as benefical bacteria is lactic acid bacteria (LAB). One of the bacteria included in LAB is probiotic bacteria. Probiotic bacteria can be isolated from several sources, one of which is breast milk. The functionality of probiotics is seen by the production of exopolysaccharides (EPS). EPS is produced through the expression of the fructosyltransferase (ftf) gene. The samples used in this study were 16 isolates of candidate probiotic bacteria from breast milk. The stages of this research included reculture of probiotic isolates, isolation of bacterial genomic DNA, amplification of isolates with 16s rRNA primers, amplification of isolates with primary lactic acid bacteria (LAB) and amplification of isolates with specific primers of ftf gene. The results showed that 10 of 16 probiotic bacterial isolates had the ftf gene indicated by the presence of DNA bands on the electrophoregram with a size 58 bp.

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