Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Sri Rianawati
Balai Penelitian Tanaman Hias
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Embriogenesis Somatik dari Eksplan Daun Anggrek Phalaenopsis sp L. (Somatic Embryogenesis from Leaf Eksplant of Phalaenopsis Orchids) Sri Rianawati; Agus Purwito; Ridho Kurniati; Budi Marwoto; Suryanah Suryanah
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 3 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (606.295 KB) | DOI: 10.24831/jai.v37i3.1241

Abstract

Somatic embryogenesis has been recoqnized as one of the process on plant micropropagation  techniques. This process occured through regeneration by direct embryo formation and through an intermediary callus phase. This research was conducted through an intermediary callus phase. The experiment was initiated with callus induction from leaf explant on five modifications of MS medium i.e :1/2MS without plant hormone  (MI-0); ½ MS containing 1mg/L BA + 0.5 mg/L 2.4-D + 1mg/L NAA  (MI-1);1/3 MS containing 2 mg/L  2.4-D (MI-2); ½ MS supplemented with 0.5 mg/L 2.4-D + 0.5 mg/L BAP +0.2 mg/L thidiazuron (MI-3); ½ MS supplemented 2 mg/L thidiazuron and 1 mg/L BAP (MI-4). After the tissues were swollen, the  explants  were  placed on callus proliferation medium  ½ MS supplemented with 0.2 mg/L thidiazuron and 0.5 mg/L 2.4-D (MP). After two months, calli were  regenerated in regeneration medium ½ MS supplemented with 0.4  mg/L BAP and 0.2 mg/L  2.4-D (MR). The results of this research  showed that  MI-1 and MI-3 were the best swelling explant mediums   before the callus  produced in both MP and MR medium. Callus produced was increased in every subculture. However, the level of callii production decreased on the following subculture. Plantlets were regenerated from somatic embryos derived from  callii on MR medium. The results of this study may contribute to our advancement of scientific knowledge achievements tissue culture techniques to support inconventional plant improvement.   Key words:  embryo somatic induction, in vitro, embryogenic callii  
Co-Authors Agus Purwito Budi Marwoto Ridho Kurniati Suryanah Suryanah