Introduction: NipahVirus infection is a newly appear animal disease that develop a serious disease in bothanimal group and human being. NiV is a contagious agent which develops the serious illnesses by the Nipah(genus Henipavirus). NiV may be spread from animal (e.g. bats or pigs) or infected foodstuffs to man andmay also be spread directly from mans to mans. Case mortality levels are reported at 40% to 75%, based onepidemiological monitoring and clinical intervention. This incidence can vary by epidemic. A self-instructionmodule on knowledge regarding Nipah virus infection and its prevention among general population helps toimprove and update the knowledge regarding Nipah virus infection. Hence the investigator felt the need todevelop a self instruction module for preventing Nipah virus infection.Aim: To determine the effectiveness of self instruction module on knowledge regarding Nipah virus infectionand its prevention among general population.Materials and Methods: A quasi-experimental research design was used to conduct this study. Nonprobability convenient sampling technique used to collect data from general population on the basis ofstructured knowledge questioner. After collecting pretest data, self-instructional module was given forintervention on knowledge regarding Nipah virus infection and its prevention among general population.Seven days were given to the samples for utilizing self instructional module which was organized for 45-50 minutes. Post-test information was gathered after seven days from the day of intervention. The samplecharacteristics were described by frequency, percentage and t-test was used to describe the differencebetween pre-test and post-test knowledge score. Chi-square test was also used to find out the associationbetween knowledge of general population regarding Nipah virus infection and its prevention with selecteddemographic variables.Result: The findings showed that in pretest 112 (93.33 %) general population had average knowledge,08(6.67%) general population had good knowledge, whereas post test 7(5.8 %) had good knowledge, 113(94.17%) had excellent knowledge.Conclusion: Nipah Virus is a newly emerging animal disease that leads to a serious disease in both animalsand humans. Nipah virus may be transmitted to mans from animals (e.g. bats or pigs) or infected foods, andmay also be transmitted directly from mans to mans so as this intervention will helpful to aware the generalpopulation to prevent Nipah virus infection and its complication.