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Altering physiological conditions and semen acidity by manipulating dietary cation-anion difference and fish oil supplemenation of Garut breed rams Hidayat, Rahmat; Toharmat, T.; Boediono, A.; Permana, I.G.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 1 (2009)
Publisher : Indonesian Animal Sciences Society

Contribution of Garut breed sheep as protein resources is not optimal yet.Â Acceleration of population growth such as increasing of female offspring is neccesary.Â This study was carried out to obtain information regarding the effect of dietary cation-anion difference (PKAR: meq [(Na + K) â (Cl+S)/100 g of dry matter]) and fish oil supplementation on acidity of blood and semen, blood gas and plasma and semen mineral. The dietary treatmens were as follows:Â Â RN0= basal ration (PKAR +14) without fish oil, RNI= basal ration (PKAR +14) with 3% fish oil, RB0= base ration (PKAR +40) without fish oil, RBI= base ration (PKAR +40) with 3% fish oil, RA0= acid ration (PKAR -40) without fish oil, and RAI= acid ration (PKAR -40) with 3% fish oil.Â The rations contained 150 ppm of zinc and were offered to 18 of Garut rams.Â The result indicated that blood pH, pCO2, and pO2 were not affected by PKAR and fish oil supplementation, but cHCO3 and cBase were affected (P<0.05).Â PKAR and fish oil supplementation affected (P<0.01) plasma Mg and S, but did not affect K, Na, Zn and Cl.Â Semen pH after day 28 of experimental period were highly significant different and that followed PKAR pattern.Â In conclution, PKAR affected cBase, cHCO3, concentration of Mg and S plasma and semen pH of Garut rams.Â The result suggested that PKAR could be applied to manipulate physiological condition and semen pH. Key Words: PKAR, Physiological Condition, Semen, Garut Ram

Performance of Garut breed rams fed diets containing various cation-anion difference with or without fish oil supplementation Hidayat, Rahmat; Toharmat, T.; Boediono, A.; Permana, I.G.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 3 (2011)
Publisher : Indonesian Animal Sciences Society

This study was carried out to evaluate the effect of dietary cation-anion difference (DCAD) and fish oil supplementations on dry matter intake (DMI), dry matter digestibility (DMD) and organic matter digestibility (OMD), weight gain, as well as the acidity of urine in Garut breed rams. The experiment was done based on randomized group design with 6 treatmentsÂ and 3 groups. The dietary treatments were as follows: R0= basal ration (DCAD +14) without fish oil, R1 = basal ration (DCAD +14) with 3% fish oil, R2 = base ration (DCAD +40) without fish oil, R3 = base ration (DCAD +40) with 3% fish oil, R4 = acid ration (DCAD -40) without fish oil, and R5 = acid ration (DCAD -40) with 3% fish oil. All rations contained 150 ppm of zinc and were offered to 18 of Garut breed rams. The results indicated that DCAD +40 and -40 decreased DMI significantly.Â The ration with DCAD +40 had the lowest DMI. Fish oil supplementation decreased DMI. No differences were observed for DMD and OMD.Â DCAD +40 and -40 decreased body weight of rams. However, the body weight of rams was very low. Variation of urine pH followed the DCAD pattern. It was concluded that DCAD +40 and -40, as well as fish oil supplementations decreased DMI, body weight and urine pH followed the DCAD pattern. Keys Words: Cation, Anion, Body Weight, Digestibility, Garut Breed Rams

In Vitro Development Of Holstein And Japanese Black Breeds Embryo A. Boediono; T. Suzuki
Media Veteriner Vol. 3 No. 1 (1996): Media Veteriner
Publisher : Media Veteriner

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Two experiments were conducted with 3,457 oocytes, aspirated from the ovaries of slaughtered Holstein and Japanese Black breeds cows to compare the use of superovulatedcow serum (SCS) and fetal calf serum (FCS) supplementation into medium for in vjtro development (experiment I) and the effect of different source of oocytes (experiment II). Theendpoint was development to cleavage on day 2 and to blastocyst up to day 9 after msermnabon. In experiment I, oocytes were matured and cultured m vltro (after fertilization)in TCM-199 supplemented with 5% SCS or 5% FCS. The cleavage rate of zygotes cultured in medium supplemented with 5% SCS was higher (P

Performance of Garut breed rams fed diets containing various cation-anion difference with or without fish oil supplementation Rahmat Hidayat; T. Toharmat; A. Boediono; I.G. Permana
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 3 (2011): SEPTEMBER 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

This study was carried out to evaluate the effect of dietary cation-anion difference (DCAD) and fish oil supplementations on dry matter intake (DMI), dry matter digestibility (DMD) and organic matter digestibility (OMD), weight gain, as well as the acidity of urine in Garut breed rams. The experiment was done based on randomized group design with 6 treatments and 3 groups. The dietary treatments were as follows: R0= basal ration (DCAD +14) without fish oil, R1 = basal ration (DCAD +14) with 3% fish oil, R2 = base ration (DCAD +40) without fish oil, R3 = base ration (DCAD +40) with 3% fish oil, R4 = acid ration (DCAD -40) without fish oil, and R5 = acid ration (DCAD -40) with 3% fish oil. All rations contained 150 ppm of zinc and were offered to 18 of Garut breed rams. The results indicated that DCAD +40 and -40 decreased DMI significantly. The ration with DCAD +40 had the lowest DMI. Fish oil supplementation decreased DMI. No differences were observed for DMD and OMD. DCAD +40 and -40 decreased body weight of rams. However, the body weight of rams was very low. Variation of urine pH followed the DCAD pattern. It was concluded that DCAD +40 and -40, as well as fish oil supplementations decreased DMI, body weight and urine pH followed the DCAD pattern. Keys Words: Cation, Anion, Body Weight, Digestibility, Garut Breed Rams

Altering physiological conditions and semen acidity by manipulating dietary cation-anion difference and fish oil supplemenation of Garut breed rams Rahmat Hidayat; T. Toharmat; A. Boediono; I.G. Permana
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 1 (2009): MARCH 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Contribution of Garut breed sheep as protein resources is not optimal yet. Acceleration of population growth such as increasing of female offspring is neccesary. This study was carried out to obtain information regarding the effect of dietary cation-anion difference (PKAR: meq [(Na + K) – (Cl+S)/100 g of dry matter]) and fish oil supplementation on acidity of blood and semen, blood gas and plasma and semen mineral. The dietary treatmens were as follows: RN0= basal ration (PKAR +14) without fish oil, RNI= basal ration (PKAR +14) with 3% fish oil, RB0= base ration (PKAR +40) without fish oil, RBI= base ration (PKAR +40) with 3% fish oil, RA0= acid ration (PKAR -40) without fish oil, and RAI= acid ration (PKAR -40) with 3% fish oil. The rations contained 150 ppm of zinc and were offered to 18 of Garut rams. The result indicated that blood pH, pCO2, and pO2 were not affected by PKAR and fish oil supplementation, but cHCO3 and cBase were affected (P<0.05). PKAR and fish oil supplementation affected (P<0.01) plasma Mg and S, but did not affect K, Na, Zn and Cl. Semen pH after day 28 of experimental period were highly significant different and that followed PKAR pattern. In conclution, PKAR affected cBase, cHCO3, concentration of Mg and S plasma and semen pH of Garut rams. The result suggested that PKAR could be applied to manipulate physiological condition and semen pH. Key Words: PKAR, Physiological Condition, Semen, Garut Ram

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