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Isolation And Identification Of Carried Seed Fungus Acacia crassicarpa A. Cunn. Ex Benth Era Kurniati Kurniati; Delita Zul; Budi Tjahyono
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol 2 No 1 (2020): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 2 No.1, 2020
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Penyakit yang disebabkan oleh cendawan pada bibit Acacia crassicarpa (Akasia) sangat berpotensi menimbulkan kerusakan pada bibit dan dapat terbawa benih. Bentuk kerusakan karena serangan patogen sangat bervariasi, bergantung pada jenis patogen, benih dan faktor lingkungan. Oleh karena itu dilakukan penelitian yang bertujuan untuk isolasi dan identifikasi patogen terbawa benih Akasia. Benih yang digunakan sebagai sumber isolat yaitu benih yang disimpan di Dry Cold Storage (DCS) dengan tahun panen benih 2012, 2015 dan 2016. Isolasi cendawan dari benih dilakukan dengan meletakkan benih di atas medium agar PPA dan dipurifikasi pada medisum PSA. Karakterisasi secara mikroskopik menunjukkan bahwa cendawan yang diisolasi terdiri dari 4 genus, yaitu Aspergillus, Rhizopus, Penicillium dan Fusarium. Isolat yang secara mikroskopis termasuk genus Fusarium diidentifikasi secara molekuler berdasarkan Internal Transcribed Spacer (ITS). Hasil BLAST menunjukkan bahwa sekuen (ITS) isolat 2012 dan 2016 dengan ukuran 570-580 bp memiliki kemiripan paling dekat dengan cendawan kelompok Fusarium sp. Nilai max identity isolat uji sebesar 88,87% (isolat 2012) dan 87,55% (isolat 2016). Hasil uji patogenisitas cendawan Fusarium sp. secara in vitro menunjukkan bahwa tidak ada gejala nekrosis yang muncul selama pengamatan. Dapat disimpulkan bahwa Fusarium yang diisolasi diduga tidak bersifat patogen.

Population Of Bacteria In Soil Dystrudepts Under Oil Palm In The Application Of Organic Mulch And Earthworm Juliarni Juliarni; Wawan Wawan; Delita Zul
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol 3 No 1 (2021): Volume 3 No.1 Januari 2021 Jurnal Agronomi Tanaman Tropika (JUATIKA)
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v3i1.415

Research on the effect of organic mulch and earthworms under oil palm plantation on the total population bacteria is still scarce. The study aims to analyze the effect of organich mulch, earthworm desity, and the interaction between organich mulch and earthworm density on the total population bacteria. The research was carried out in May – August 2019. This research was a 3 x 4 factorial experiment which was placed according to a Split Plot Design with the basic design of Randomized Block Design, each treatment was repeated 3 times. Organic mulch as the main plot are 3 type, namely: M1 (M. bracteata), M2 (oil palm empty fruit brunches/ OPEFB) and M3 (oil palm midrib). The density of earthworms as subplots consists of 4 levels, namely: C0 (0 worms/m2), C1 (35 worms/m2), C2 (50 worms/m2) and C3 (65 worms/m2). The data were analyzed using analysis of variance. If the treatment is significantly different, the treatment was tested by Duncan’s at 5% significance level. The results showed that oil palm midrib organic mulch can increase the total population of fungi and bacteria compared to M. bracteata and OPFEB. The earthworms density 50 and 65 worms/m2 can increase the total bacterial population compared to other earthworm densities. The combination of oil palm midrib with earthworms density 50 and 65 worms/m2 can increase the total population of bacteria

FORMULASI BIOFERTILIZER CAIR MENGGUNAKAN BAKTERI PELARUT FOSFAT INDIGENUS ASAL TANAH GAMBUT RIAU Suci Novri Yelti; Delita Zul; Bernadeta Leni Fibriarti
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Inefficient of P-uptake by plants during the application of chemical phosphorus (P) fertilizer results P-leaching to the aquatic environment. Therefore, the use of phosphate solubilizing bacteria (PSB) to enhance solubilization of P is necessary. The aims of this study were to find the best formulation in producing liquid biofertilizer which contained PSB and to analyze the storage time of liquid biofertilizer which were produced. As many as 4 selected PSB isolates (BB_UB6, BB_K9, BB_K2 and BB_HS13) were used to produce 3 combination starters. Liquid biofertilizers were produced by fermentation using three types of formulation, namely Pikovskaya's medium, coconut water enriched with 2% molasses, and tofu waste water. The quality of liquid biofertilizer was determined by calculating the PSB cells number during 0, 30, and 60 days of storage time and by measuring the liquid biofertilizer acidity. Liquid biofertilizers produced were kept at room and refrigerator temperature. The results showed that the PSB cell numbers of starter I, II and III were higher in liquid biofertilizer that was formulated with coconut water which containing 2% of molasses until 60 days of storage time. The cell numbers of PSB ranged from 7,0×1010 - 2,82×1011 CFU/ml. In general, the PSB cell number was relatively stable when liquid biofertilizer was kept at room temperature. Based on this results, it can be concluded that the best formulation to produce liquid biofertilizer was the coconut water enriched with 2% molasses.

Isolasi Bakteri Indigenus yang Potensial Sebagai Agen Biofertilizer Asal Tanah Gambut di Kawasan Zamrud dan Taman Nasional Tesso Nilo, Riau Rohyani '; Delita Zul; Bernadeta Leni Fibrianti
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Biofertilizer is a substance of functional microorganisms that has a role in providing nutrient for plant and can be used as a substitute for chemical fertilizer. This functional microorganisms such as phosphate solubilizing microorganisms, cellulolytic microorganisms, and nitrogen fixing microorganisms are rich in soil. The purpose of this study was to isolate the indigenous bacteria that are potential as biofertilizer agent. The group fuiontional bacteria was isolated from peat soil in the Zamrud forest and the Tesso Nilo National Park (TNTN) by growing them on suitable selective media. Symbiotic nitrogen fixing bacteria, non symbiotic nitrogen fixing bacteria, phosphate solubilizing bacteria (PSB), and cellulolytic bacteria were isolated using of yeast extract mannitol agar (YEMA) medium, Asby’s mannitol phosphate and NFb media, pikovskaya's medium, and cellulose congo red agar (CCRA) medium, respectively. The potential of isolated bacteria as biofertilizer agent was analyzed qualitatively based on size of clear zone formed around colony of PSB and cellulolytic bacteria, whereas symbiotic nitrogen fixing bacteria were selected by its ability to absorb congo red and non symbiotic nitrogen fixing bacteria were chosen based on its ability to form pellicle and change of medium color. As manys as 170 isolates were succesfully obtained, consisted of 77 isolates of symbiotic nitrogen fixing bacteria, 47 isolates of symbiotic nitrogen fixing bacteria, 74 of PSB, and 19 isolates of cellulolytic bacteria. The potential PSB and cellulolytic bacteria isolated were isolate of FTS1-2AS1 and SZ1-2B2, respectively that showed the highest value of ratio clear zone and diameter colony.

SELEKSI KEMAMPUAN BAKTERI PELARUT FOSFAT ASAL BUKIT BATU-RIAU DALAM MENGHASILKAN ASAM SIANIDA Pristiana Aprillia; Delita Zul; Bernadeta Leni Fibriarti
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Phosphate solubilizing bacteria (PSB) is beneficial bacteria which are not only capablein solubilizing organic phosphate into inorganic phosphate, but also producing cyanideacid (HCN). HCN plays a role in controling weed growth. The objective of this researchwas to screen the potency of indigenous PSB isolated from peat soil in producting HCN.The collections of PSB were subcultured in King’B broth. The ability of 152 isolates onHCN production were tested qualitatively using filter paper soaked with picric acid andNa 2 CO 3 . The result indicated that as many as 26 isolates (17.1%) were able to releasevarious levels of HCN production based on the appearing of brown, dark brown, orreddish brown colour on the filter paper.

UJI EFEKTIVITAS BAKTERI PELARUT FOSFAT PENGHASIL ASAM SIANIDA ASAL TANAH GAMBUT RIAU DALAM MENGENDALIKAN GULMA DOMINAN PADA TANAMAN KELAPA SAWIT Tri Septiani; Delita Zul; Mayta Novaliza Isda
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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This research aimed to analyze the effectiveness of phosphate solubilizing bacteria (PSB) which are able to produce cyanide acid (HCN) in controlling of predominant weed in the palm oil plantation. This research was conducted from February to July 2014 in the Laboratory of Microbiology, Faculty of Mathematics and Natural Science, University of Riau. The effectiveness of PSB in controlling the growth of Mikania micrantha was performed by inoculating 26 selected PSB isolates into pre-germinated weed based on the Kremer and Souissi method modification. The parameters observed were root length and shoot length of seedling weed. The results showed that PSB isolates tested, in general, were effective to inhibit the growth of root and shoot length of the weed. BB_K9 isolate was the most effective in controlling the weed as it had a strong level of HCN production.

ENUMERASI TOTAL POPULASI MIKROBA TANAH GAMBUT DI TELUK MERANTI KABUPATEN RIAU Rahmi Fitria; Delita Zul; Bernadeta Leni F
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 1 (2014): Wisuda Februari 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Teluk Meranti is one of the peatland area in Riau province. Most of these lands have beenchanged into palm oil plantation, timber plantation, agricultural area and settlement. Theaim of this research was to analyze the impact of land use changes on soil physical-chemical characteristics and microbial cell number. Soil samples were taken from eightdifferent locations, namely primary forest as control, secondary forest, rubber plantation(15 monthsyears old), rubber forest (40-60 years old), palm oil plantation (7-8 years old),acacia plantation (2-3 years old), corn field, and cassava field. Microbial cell number wasdetermined by spread plate method, employing appropriate media for the growth ofbacteria, fungi and actinomycetes. The results showed that the soil humidity, soiltemperature, percentage of soil dry weight, water content, soil bulk density and pH rangedfrom 29,63-55,88%, 27-31,5 o C, 14,9-35,5%, 64,9-85,1%, 0,16-0,39 g/cm 3 and 3,63-4,00,respectively. The copiotrophic bacterial cell number ranged from 0,6x10 5 -1,8x10 5 CFU/gsoil where the highest population was at the palm oil plantation,whereas the oligotrophicbacterial cell number ranged from 0,5x10 5 -1,4x10 5 CFU/g soil where the highest populationwas at the palm oil plantation. The population of fungi ranged from 0,4x10 5 -1,0x10 5 CFU/gsoil where the highest population was at the corn field. The population of actinomycetesranged from 0,4x10 5 -10,7x10 5 CFU/g soil where the highest population was at the palm oilplantation. Land use changes caused microbial cell number increased. The results indicatedthat land use changes influenced the microbial cell numbers.

APLIKASI MIKROBA LIGNOSELULOLITIK INDIGENUS ASAL TANAH GAMBUT RIAU DALAM PEMBUATAN KOMPOS DARI CAMPURAN TANDAN KOSONG DAN LIMBAH CAIR PABRIK KELAPA SAWIT (Elaeis guineensis Jacq.) Happy Zatul Munawarah; Delita Zul; Bernadeta Leni Fibriarti
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Activity of palm oil industries usually has side result such as empty fruit bunch (EFB) and palm oil mill effluent (POME) wastes. Those wastes still contain organic material that can be utilized as a substrate for compost production. Because of lignin and cellulose components, composting of EFB takes about 60 days to obtain standardized composts. It is known that composting process can be shorterned by adding bioactivators. The purpose of this research was to analyze the ability of indigenous lignocellulolytic microbes isolated from peat soil in Riau as bioactivator in composting of EFB enriched by POME. The selected isolates consisting of 4 bacteria (BB_S27, BB_HP42, BB_HP41 and BB_K20) and 2 fungi (LIJ1 and L1J2) were subcultured on Nutrient Broth and Potato Dextrose Broth. Starters were then made from a combination of the isolates and fermented during 7 days using seedling media. The compost treatments included negative control (seedling media without isolates), positive control by using effective microorganisms (EM) and bioactivator made in USA, and 4 combinations of those isolates resulting 7 treatments. Composting was done by the windrow composting system utilizing 400 kg EFB as a substrate and inoculated by 50 liters of the starters at the 1st and 14th incubation time. During 35 days composting process, the substrate was enriched by 20 liters POME every 2 days. The best quality compost was produced by treatment K3 (a combination of 4 bacteria and 2 fungi) as its characters almost in line with the National Quality Standard (ISO) such as N 2.22%, C/N ratio 14.5, P 0.760%, K 3.44%, blackish brown color, smell like soil and unraveled texture. The highest celullolytic and ligninolytic microbes cell number were also found in compost treated by K3 with value 8.8 x 108 CFU/g and 1.2 X 109 CFU/g, respectively.

FORMULASI PUPUK HAYATI SERBUK MENGGUNAKAN BAKTERI PELARUT FOSFAT INDIGENUS ASAL TANAH GAMBUT RIAU DALAM BERBAGAI BAHAN PEMBAWA Dewi Haryanti; Delita Zul; Bernadeta Leni Fibriarti
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Peat soil has been known for its low content in phosphor (P). On the other hand, P solubilizing process is naturally slow. Inoculation of phosphate solubilizing bacteria (PSB) could increase this process. Therefore, the exploration of bacteria with such potential for use as biofertilizer agents is needed. Quality of biofertilizer depends on the viability and amount of bacteria contained, it is also influenced by type of carrier material. This research was aimed to find the appropriate carrier materials in production of biofertilizer which were contained PSB agent and to determine the quality of biofertilizer produced at a certain storage time. As many as 4 selected PSB isolates (BB_UB6, BB_K9, BB_K2, and BB_HS13) were used to produce 3 starter combinations. The starters were prepared by growing the isolates in Pikovskaya’s media. Biofertilizer was produced by inoculating each starter into the peat and wood charcoal as carrier material and was fermented for 4 days. Biofertilizer quality was determined by calculating the PSB cells number during 0, 30, 60, and 90 days storage time and the degree of biofertilizer acidity. The cell numbers of starter I, II, and III ranged from 8.2.1010-2.9.1011 CFU/g, 5.3.1010-2.9.1011 CFU/g, and 8.0.1010-2.9.1011 CFU/g, respectively in a period of storage 0-90 days. The highest cell number at the end of storage was found from biofertilizer produced by using peat, as carrier material which was kept at 40C (1,3.1011 CFU/g, starter I), (8.6.1010 CFU/g, starter II), and (9.6.1010 CFU/g, starter III). Biofertilizer acidity was relatively neutral, 6.38. Based on the results obtained, peat was revealed as a good carrier material and the quality of biofertilizers which were produced was still good.

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