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Septi Anitasari

Universitas Brawijaya

Author-ID : 2435922

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

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Kajian Efek Paparan Exponential Dan Square Wave dalam Metode Elektroporasi Terhadap Motilitas Dan Viabilitas Sperma Ikan Nilem (Osteochilus hasselti) abd raheem raheem faqih; Septi Anitasari; Angga Wiratama
Journal of Aquaculture and Fish Health Vol. 8 No. 3 (2019): JAFH vol. 8 no. 3 September 2019
Publisher : Department of Aquaculture

Tujuan penelitian ini adalah untuk mengetahui pengaruh perbedaan metode (Square wave dan Eksponensial) terhadap pergerakan (motilitas) dan kemampuan hidup (viabilitas) sperma ikan nilem (Osteochilus hasselti). Dengan harapan nantinya dapat menjadi dasar pijakan dalam transfer gen pada ikan nilem (Osteochilus hasselti) dengan menggunakan metode elektroporasi, dengan sperma ikan sebagai media transfer gen. Metode transfer gen sejenis ini masih belum dilakukan di Indonesia. Umtuk itu penelitian sperma yang digunakan sebagai media untuk memasukkan DNA asing ke dalam sel telur perlu dilakukan. Salah aspek yang perlu dikaji antara lain penelitian tentang pengaruh medan listrik dengan metode yang berbeda terhadap motilitas dan viabilitas sperma ikan.Penelitian ini dilaksanakan di Laboratorium Breeding (Budidaya Perairan) Fakultas Perikanan dan Ilmu Kelautan serta di Laboratorium Sentral Ilmu Hayati (LSIH) Universitas Brawijaya Malang. Metode penelitian yang digunakan adalah metode eksperimen, sedangkan untuk analisa data digunakan adalah Uji t karena terdiri dari 2 perlakuan dengan 3 kali ulangan. Sebagai perlakuan adalah metode Square wave dan eksponensial juga dilakukan 2 kontrol yaitu pengamatan sperma sebelum dan sesudah perlakuan. Parameter utama dalam penelitian ini adalah motilitas dan viabilitas sperma ikan nilem. Hasil penelitian menunjukkan bahwa elektroporasi dengan metode Square Wave lebih baik hasilnya jika dibandingkan dengan metode Eksponensial. Hal tersebut dapat dilihat pada nilai rata-rata motilitas sperma ikan nilem dengan menggunakan metode square wave sebesar 34%, sedangkan dengan metode eksponensial sebesar 11%. Demikian pula hasil nilai rata-rata viabilitas sperma ikan nilem dengan menggunakan metode Square Wave sebesar 56%, sedangkan dengan menggunakan metode Eksponensial sebesar 25%. Hasil pengamatan sperma sebelum elektroporasi diperoleh nilai motilitas sebesar 71,3% dan viiabilitas sebesar 75%, sedangkan sesudah elektroporasi diperoleh nilai motilitas sebesar 46,6% dan viabilitas sebesar 52,6%.

Phylogenetic Study of Lempuk Fish (Gobiopterus sp.) in Ranu Grati Based on Mitochondrial DNA Sequence of Cytochrome Oxidase I (COI) Barcoding Region Abd Rahem Faqih; Wahyu Endra Kusuma; Septi Anitasari; Dian Kartika Sari
Journal of Aquaculture and Fish Health Vol. 10 No. 3 (2021): JAFH Vol. 10 No. 3 September 2021
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v10i3.23137

Lempuk fish (Gobiopterus sp.) is one of the local species in Ranu Grati. The high level of Lempuk fishing and the low level of awareness of Grati community on the importance of conservation and domestication efforts, as well as the taxonomy of the fish itself have threatened the population. Therefore, long-term efforts should be undertaken to preserve and identify the origin of Lempuk fish by conducting a molecular phylogenetic study based on COI. This study aimed to determine, analyze and describe the kinship and phylogenetic relationships of Lempuk fish (Gobiopterus sp.). This study used a descriptive method, starting from the specimen collection, extraction by the Phenol-Chloroform method and amplification by the PCR method, to the screening process. The data analysis method used the MEGA 5 application. Phylogenetic study used the Maximum Likelihood method with a bootstrap value of 1.000 times. The results showed that the intra-population genetic distance of Lempuk fish is low at 0.00%. Genetic distance between the population of Lempuk fish is relatively high with G. brachypterus from Malaysia of 22.4%, Central Java of 21.5%, and India of 21.4% and with G. lacustris from Malaysia of 22.5%, the Philippines of 21.6% %, Thailand of 19.7%, and with G. chuno and G. semivestitus of 21.2% and 23.6% accordingly. The phylogenetic study obtained showed the formation of a separate group that is different between Lempuk fish group and other Gobiopterus fish, supported by a bootsrap value of 99%.

The Effectiveness of hrGFP Gene Reporter Role in Carp Fish (Cyprinus carpio) Transgenesis Process Based on Convocal Microscopy Analysis Septi Anitasari; Agus Soeprijanto; Abd. Rahem Faqih
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

Sperm cells can be used as a medium to insert foreign DNA into the egg. Electroporation humanized renilla reformis Green Fluorescent Protein (hrGFP) gene as a reporter gene is very important to know the effectiveness of gene transfer. Research is aimed to understand the effectiveness rate of hrGFP gene reporter to detect the successful gene transfer process based on the result of observation with convocal microscope over transgenic sperm level, embryo and larva of Carp Fish. Result of trans-genesis of hrGFP as sperm-mediated gene reporter must be accurately detected. Based on the result of observation with convocal microscope, it is shown that hrGFP enters the core level, indicates by PI pigmentation and thereby, hrGFP gene reporter can provide information about hrGFP fluorescence starting from sperm, embryo and larva of Carp Fish.Keywords: Carp Fish, effectiveness, gene reporter, hrGFP, sperm fluorescence.

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