<![CDATA[During endodontic procedures, along with mechanical instrumentation, irrigants are imperative for pathogeneradication. As the strains of pathogens are becoming antibiotic resistant at a constant ascending pace andalso the potentially harmful effect is associated with synthetic drugs, so studies on natural alternatives forirrigation are being done. Chitosan, a natural alternative, has been evaluated as an antimicrobial medicine.Recently, in cases presenting with persistent infections after endodontic treatment, it was noted that of all themicrobial species identified, the prevalence of s.epidermidis was the most. In this current study, an analysisof the antimicrobial effectiveness of chitosan as a potent root canal irrigant, for disinfecting the root canalwas conducted. Methodology: By the serial dilution method, the minimum inhibitory concentration wasdetermined. Dilution of the test solution was done using 10% DMSO solution. After which, incubationin a rotary incubator, at 180 rotations per minute, the temperature of 37?, for 24 hours was done. Forobtaining the zones of inhibition of different concentration (0.2%, 0.5%, 0.75, 1%) of chitosan irrigant, theagar diffusion method was applied and the resultant clear zone was measured with a ruler. Result: Chitosan(liquid) had promising antibacterial activity against Staphylococcus epidermidis, with a 16 mm zone ofinhibition at 100 µg/ml. Conclusion: Within the limitations of the present study, it was observed that, evenat lower concentrations, chitosan (liquid form) effectively hindered the further growth of S.epidermidis.]]>
