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All Journal PROSIDING SEMINAR NASIONAL Jurnal Biomedika
Wildiani Wilson
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Potensi Antibakteri Isolat Bakteri Endofit dari Tanaman Purwoceng (Pimpinella pruatjan Molk.) Terhadap Klebsiella pneumoniae dan Salmonella typhi Wildiani Wilson; Dewi Yuniliani
Biomedika Vol 12 No 1 (2019): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1094.338 KB) | DOI: 10.31001/biomedika.v12i1.430

Abstract

This study aims to determine the isolates of purwoceng endophytic bacteria as antibacterial. Antibacterial testing was carried out using the well diffusion method. Potential endophytic bacterial isolates were first inoculated into liquid fermentation media for 52 hours and then extracted using n-hexane solvent. This crude extract is used for antibacterial testing against K. pneumoniae and S. typhi. The antibacterial activity of endophytic bacteria isolates using MHA (Mueller Hinton Agar) media with a thickness of 6 mm and a well diameter of 5 mm. The results showed that the isolates of purwoceng endophytic bacteria could not inhibit the growth of K. pneumoniae and S. typhi.
PERBANDINGAN EFEK EKSTRAK BUAH ALPUKAT (Persea americana Mill) TERHADAP PERTUMBUHAN BAKTERI Pseudomonas aeruginosa DENGAN METODE DISK DAN SUMURAN Sri Dewi Haryati; Sri Darmawati; Wildiani Wilson
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.019 KB)

Abstract

This study aimed to analyze the concentration of avocado fruit extracts method disc and wells 10% b/v, 20% b/v, 30% b/v, 40% b/v and 50% b/v in inhibited P.aeruginosasa. The avocado fruit is extracted using the maceration method. Then the activity of avocado fruit to the growth of P.aeruginosa using the method of disc and wells.The results showed the extract of avocado method of disk and wells at concentration10% b/v, 20% b/v, 30% b/v, 40% b/v and 50% b/v can inhibit P.aeruginosa bacteria the mean inhibitory zone of the disc method was 16.6 mm, 21.6 mm, 26.0 mm, 28.4 mm and 29.6 mm, as well as in the method of wells obtained mean inhibition zone respectively that is 25.4 mm, 27.4 mm, 28.8 mm, 30.2 mm and 31.2 mm.The positive control used was ciprofloxacin concentration of 25 μg forming a 33 mm inhibitory zone diameter. One Way ANOVA test was obtained p=0,000  which shows that there is an average difference between the inhibitory zone diameter between concentrations 10% b/v, 20% b/v, 30% b/v, 40% b/v and 50% b/v, as well as there is a marked difference between the disk method and the extraction of avocado extract on the growth of P.aeruginosa.The result shows that the average of the bacterial inhibition zone with the disk method is lower than the well method, the higher the concentration of avocado extract, the higher the inhibitory effect on the growth of P.aeruginosa bacteria. Keywords: Avocado Fruit Extracts, P.aeruginosa, Method Disc, Methode Wells
Co-Authors Dewi Yuniliani Sri Darmawati Sri Dewi Haryati