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Dicky Andiarsa
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Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Deteksi Diarrhoegenic E. coli pada Sampel Feses Penderita Diare di Puskesmas Batulicin dan Pagatan Kabupaten Tanah Bumbu dengan Metode Polymerase Chain Reaction (PCR) Ika Setianingsih; Dicky Andiarsa; Erli Hariyati
Biomedika Vol 12 No 2 (2019): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (392.907 KB) | DOI: 10.31001/biomedika.v12i2.597

Abstract

Diarrhoegenic E. coli (DEC) is a group of pathogenic E. coli strains known to be one of the causes of diarrheal disease. Diarrhea cases are still quite high in developing countries like Indonesia. Tanah Bumbu Regency, especially Batulicin and Pagatan, have a fluctuating rate of diarrhea. This study aims to detect DEC in diarrheal patients in the work area of ​​Batulicin and Pagatan Health Centers using the PCR examination method. Cross Sectional research design with a total sample of 15 respondents collected for 3 months (August - October 2019). Stool samples were examined using the PCR method to detect the presence of DEC strains namely EAEC, ETEC, and EPEC and EHEC. Three out of fifteen (20%) samples were known to be positive for Diarrhoegenic E. coli (DEC). ETEC strains were found in 2 (0,13%) sample, each from the Batulicin Health Center and the Pagatan Health Center, while EPEC and EAEC strains were found in the same 1 (0,07%) sample from the Pagatan Health Center. EHEC strain was not detected in all samples. Diarrhogenic E. coli strains found in this study are EAEC, ETEC, and EPEC. However, it cannot be ascertained yet that the three strains are the cause of the diarrheal disease, it needs to be further to do comprehensive research. The community should be able to increase awareness of the importance of clean and healthy behavior, especially food and beverages consumption, so it can reduce the number of diarrhea cases.
Aplikasi IJEN (Infeksi Jamur Entomopatogen pada Nyamuk) : Jamur Metarhizium anisopliae pada Nyamuk Aedes aegypti Liestiana Indriyati; Salamiah Salamiah; Lutfhi Fatah; Eko Suhartono; Muhammad Rasyid Ridha; Abdullah Fadily; Paisal Paisal; Dicky Andiarsa
Jurnal Vektor Penyakit Vol 13 No 1 (2019): Edisi Juni
Publisher : Balai Penelitian dan Pengembangan Kesehatan Donggala, Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.914 KB) | DOI: 10.22435/vektorp.v13i1.893

Abstract

Abstract Dengue hemorrhagic fever is one of the main problems in Indonesia, its handling on chemical insecticides with insecticide resistance constraints that can inhibit vector control efforts. Entomopathogenic fungi especially Metarhizium anisopliae is one of bioinsecticides that has been widely used in agriculture for controlling insect attacks, effective in killing mosquitoes, safe for vertebrates, humans and the environment and has a small risk of resistance. Appropriate methods of formulation and application for M. anisopliae infection to mosquitoes suitable for mosquito bionomic and fungus characteristics are required. Experimental research with complete randomized design was conducted to test the efficacy of. M.anisopliae solution mixed olive oil and honey formula applied to ovitrap, plant trap and black cotton trap in infecting adult female Ae. aegypti. The death of Ae.a egypti and fungal growth on kadaver was observed everyday. 100% Ae. aegypti death was obtained in the ovitrap method with mixed formulation of M.anisopliae and olive oil. This method was also capable of infecting Ae.aegypti in various phases of both adults, eggs and larvae so that in this study ovitrap olive oil method was the most effective method for infecting M.anisopliae on Ae.aegypti. Abstrak Demam berdarah dengue merupakan salah satu masalah utama di Indoneisa. Penggunaan insektisida kimia sebagai upaya pengendalian DBD saat ini memiliki kendala resistensi insektisida yang dapat menghambat upaya pengendalian vektor. Jamur entomopatogen khususnya Metarhizium anisopliae adalah salah satu bioinsektisida yang telah digunakan secara luas di bidang pertanian untuk pengendalian serangan serangga efektif membunuh nyamuk, aman bagi vertebrata, manusia dan lingkungan serta memiliki risiko resistensi yang kecil. Diperlukan metode yang tepat baik dari formulasi maupun cara aplikasi untuk penginfeksian M.anisopliae kepada nyamuk yang sesuai dengan bionomik nyamuk dan karakteristik jamur. Penelitian eksperimental dengan rancangan acak lengkap dilakukan untuk menguji efektivitas formula M.anisopliae. Larutan M.anisopliae dicampur minyak zaitun dan madu yang diaplikasikan pada ovitrap, plant trap dan black cotton trap dalam menginfeksi Ae.aegypti betina dewasa. Pengamatan dilakukan pada kematian Ae.aegypti dan pertumbuhan jamur pada kadaver setiap hari. Kematian Ae.aegypti 100% didapatkan pada metode ovitrap dengan formulasi campuran M.anisopliae dan minyak zaitun. Metode ini juga mampu menginfeksi Ae.aegypti pada berbagai fase baik dewasa, telur maupun larva sehingga pada penelitian ini metode zaitun ovitrap dinyatakan metode yang paling efektif untuk penginfeksian M.anisopliae pada Ae.aegypti.
Co-Authors Abdullah Fadily Eko Suhartono Erli Hariyati Ika Setianingsih Liestiana Indriyati Lutfhi Fatah Muhammad Rasyid Ridha Salamiah .