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All Journal Jurnal Biomedika Indonesian Journal of Science and Education
Lilis Puspa Friliansari
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health

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The Potential of Iodine Lactoglycerol as an Alternative Fungal Stain in the Laboratory Lilis Puspa Friliansari; Erick Khristian; Sofie Agustina Hermawanti
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.748

Abstract

Fungi are eukaryotic and opportunistic organisms that can cause damage and disease. Fungi can be identified by direct staining. The composition of the dyes used in fungi staining are toxic, mutagenic and harmful to laboratory personnel and the environment. Consequently, it is necessary to consider use fungi dye continuously, so its need alternative safety dyes to identify fungi in the laboratory. This research aims to determine the potential of iodine lactoglycerol with different concentrations (1.25%, 2.5%, and 5%) as an alternative dye for fungi in the laboratory. The research method used experimental design. The samples originate from moldy bread and made 27 of microscopic slides. The microscopic slides were observed with objective 40x magnification. The parameters assessed in this study were the structure and morphology of fungi including hyphae, conidiophores, vesicles, sterigmata and conidiospores and color intensity using the image analysis of ImageJ. The results showed that various concentrations of iodine lactoglycerol can staining the structure and morphology of the fungi properly and clearly. The value of color intensity of each concentration measured has a different average. The highest average value of color intensity concentration was 5% and the lowest was 1.25%. However, the result of ANOVA test showed a significance of 0.380 (P> 0.05), which means that is no difference significantly from three of various concentrations. The result showed that the difference concentration of iodine lactoglycerol does not affect the color intensity. The conclusion of this study was iodine lactoglycerol solution can be used as an alternative dye or Lactophenol-cotton Blue (LPCB) substitute for fungi identification. It is recommendation for further research be carried out regarding the decrease in the concentration of alternative color solutions below 1.25%, the incubation time for the preparation of the preparations, and the color stability of the prepared mushroom preparations.
Pisang Nangka (Musa paradisiaca) As An Alternative Media For Aspergillus niger Growth Firdha Rachmawati; Budiman; Sisna Imalda; Lilis Puspa Friliansari
Indonesian Journal of Science and Education Vol. 7 No. 2 (2023): Indonesian Journal of Science and Education
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/ijose.v7i1.231

Abstract

Nangka banana (Musa paradisiaca) is one of the most popular bananas in Indonesia. Therefore, the production of waste will increase along with the use of bananas. Nangka banana peel and flesh can be used as an alternative media for mushroom growth because of its high carbohydrate content. The purpose of this study was to observe the growth of Aspergillus niger on alternative media made from nangka banana with concentrations of 8%, 9%, 10%, 11% and 12% and to determine the optimum variation and concentration of alernative media. Aspergillus niger was grown on nangka banana flesh media, nangka banana peel media and mix of nangka banana peel and flesh as well as PDA (Potato Dextrose Agar) media as a positive control using the single dot method. Colony diameter and pigmentation were observed for five days. The variation of nangka banana mix media with a concentration of 12% is the optimum variation and concentration to grow Aspergillus niger. Keywords: Alternative media, Aspergillus niger, nangka banana
Co-Authors Budiman Erick Khristian Firdha Rachmawati Sisna Imalda Sofie Agustina Hermawanti