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All Journal Jurnal Sain Veteriner Jurnal Penelitian Saintek Jurnal Ilmu Dasar Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BIOEDUKASI Sciscitatio : Journal for Biological Science Environmental Science and Engineering Conference Proceeding Borneo Journal of Biology Education (BJBE) COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Journal of Health and Nutrition Research Journal of Comprehensive Science SERVIRISMA: Jurnal Pengabdian kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Bioma : Jurnal Ilmiah Biologi
TRI YAHYA BUDIARSO
Biology Department, Universitas Kristen Duta Wacana, Jalan Dr Wahidin Sudirohusodo 5-19, Yogyakarta 55224, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : Sciscitatio : Journal for Biological Science

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Detection and Identification of Microbial Contaminant in Bakery Products in Yogyakarta City, Indonesia Ferdian, Evelyn; Ariestanti, Catarina Aprilia; Budiarso, Tri Yahya
SCISCITATIO Vol 1 No 1 (2020): Volume 1, Number 1, January 2020
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2020.11.13

Abstract

Bread has been chosen as an alternative food because of its availability. In Indonesia, consumptionof breads is increased due to the change of diet pattern into packed and ready-made meals. Therefore, it isimportant to raise the awareness of bakery products quality. The purpose of this study was to detect andidentify the microbial contaminant in bakery products in Yogyakarta City, Indonesia. Bacterial coloniesfrom expired bakery products were isolated into pure isolate then confirmed by API Staph and PolymeraseChain Reaction (PCR) method. The results showed there were five kinds of gram-positive bacteria. Isolatedbacteria identified by API were Bacillus cereus (52.8%), Bacillus subtilis (97.7%), Staphylococcus aureus (97.7%),Staphylococcus epidermidis (97,9%) and Staphylococcus saprophyticus (72,2% and 61,8%).
Isolation and Identification of Biofilm-Forming Staphylococcus Aureus in Commercial Cow Milk Products Yambise, Dominggas; Ariestanti, Catarina Aprilia; Budiarso, Tri Yahya
SCISCITATIO Vol 1 No 2 (2020): Volume 1, Number 2, July 2020
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2020.12.33

Abstract

Food poisoning caused by the contamination from Staphylococcus aureus are frequently found in foodespecially in dairy products. Pasteurization process in milk production was not enough to kill S. aureus becauseit formed biofilm that could survive in high temperature. This research aimed to study the presence of biofilmforming S. aureus in samples from packed commercial milk products in Yogyakarta City, Indonesia. Twentyisolates from dairy products were grown in Brain Heart Infusion (BHI) broth then inoculated into Braid-ParkerAgar (BPA) medium to get the candidate of S. aureus isolates. These isolate candidates were selected usingMannitol Salt Agar (MSA) and Congo Red Agar (CRA) medium. Another selection was done by carbohydratefermentation analysis and confirmed using API STAPH. Confirmation analysis showed that eight isolateswere identified as S. aureus. Another two isolates were identified as S. xylosus and S. haemolyticus. Therefore,it indicated the presence of Staphylococcus aureus as contaminant in dairy products.
Co-Authors -, Tri Wahyuni Sekar Sari Andriani, Eunike Marganingrum Ariestanti, Catarina Aprilia Catarina Aprilia Ariestanti Charis Amarantini CHARIS AMARANTINI Ferdian, Evelyn Govanni, Patricia Guruh Prihatmo Hutri Catur Sad Winarni Lifiana, Aulia Pare, Regina Londong Pareakan, Jefyne Mali’ Rangga, Febby Alvanda RATNA SETYANINGSIH Safriana Nata Wijaya Samodra, Eunike Andriani Marganingrum Suryanto, Regina Tri Wahyuni Sekar Sari - Vico, Yoel Wattimury, Angelia Yambise, Dominggas