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All Journal Jurnal Penelitian Saintek Jurnal Ilmu Dasar Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi BIOEDUKASI Sciscitatio : Journal for Biological Science Berkala Penelitian Hayati Jurnal Abdi Masyarakat Indonesia COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Journal of Health and Nutrition Research Journal of Comprehensive Science SERVIRISMA: Jurnal Pengabdian kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Bioma : Jurnal Ilmiah Biologi
CHARIS AMARANTINI
Biology Department, Universitas Kristen Duta Wacana, Jalan Dr Wahidin Sudirohusodo 5-19, Yogyakarta 55224, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Profil Cemaran Bakteri Coliform pada Minuman Susu Segar yang Dijual Pedagang Kaki Lima di Daerah Istimewa Yogyakarta Amarantini, Charis; Budiarso, Tri Yahya; Suryanto, Regina
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2005): February 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.539 KB) | DOI: 10.24002/biota.v10i1.2836

Abstract

Coliform contamination emerges public health case particularly by pathogenic E.coli O157 which is characterized by hemorrhagic colitis with diarrhea. In order to study the profile of coliform bacteria contamination in fresh milk that was sold by the sidewalk trader in Yogyakarta province, in this research coliform detection was done by cultivate fresh milk samples on the medium of Chromocult Coliform Agar (CCA) and enrichment cultures on the medium of Vancomycin-Trypticase Soy Broth (mVTSB). Dark-blue colonies isolated on CCA plates were then evaluated for the ability to ferment D-sorbitol on EOH medium in order to suspect pathogenic E.coli O157 colonies. The results show that coliform contamination was 1.103 - 1.107 CFU/ml, although it was not detected on all of the samples. Profile of coliform contamination in fresh milk samples consisted of Citrobacter, Enterobacter, and Klebsiella (1,0.103  - 1,2.107 CFU/ml), Shigella, Salmonella, and Yersinia (1,0.103 - 2,1.106 CFU/ml), and E.coli (1,4.104 - 2,8.104 CFU/ml). Dark-blue isolates that were suspected as pathogenic E.coli do not ferment D-sorbitol on EOH medium. Based on this result, it was concluded that it was not associated with E.coli O157.
ISOLASI, KARAKTERISASI DAN PENGELOMPOKAN STRAIN Salmonella typhi ASAL KABUPATEN SUMBA BARAT DAYA NUSA TENGGARA TIMUR BERDASARKAN SIFAT-SIFAT FENOTIP Charis Amarantini; Langkah Sembiring; Haripurnomo Kushadiwijaya; Widya Asmara
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 2 (2009): June 2009
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/339

Abstract

Typhoid fever is highly endemic in the South-West Sumba Regency, East Nusa Tenggara. The incidence rate of the diseases is high estimated at 725/100,000. It is an acute systemic infection caused by Salmonella typhi. The clinical symptoms of the disease are extremely diverse, starting from the mild form to severe ones with the most feared complication being perforation within the small intestine. Therefore, it is important to perform isolation, characterization, and grouping of S. typhi strains from the blood culture in order to determined definitely diagnosis and the different phenotypic characteristics in the community. Isolation was done in selective and differential media: BacT/ALERT FA culture media, Selenite Cystine Broth, Chromocult Coliform Agar, MacConkey Agar, and Salmonella Shigella Agar. The typical colony of Salmonella was confirmed on Triple Sugar Iron Agar, Urea agar, and L-Lysine decarboxylation media. Phenotypic characteristics of all isolates were identified using API 20E and API 50CHE diagnostics. Based on biochemical characteristics the result showed that 18 strains obtained from different geographical origins were diverse. Four strains have similarity value 100% while the remained strains have similarity value 86.3–98.4%. All of the strains were categorized in the species of S. typhi.
Pemberdayaan Ekonomi Umat : “Pemanfaatan Lingkungan Disekitar Rumah Untuk Budidaya Bayam Brazil Di Era Pandemi” Tri Yahya Budiarso; Charis Amarantini; Guruh Prihatmo
Servirisma Vol. 2 No. 1 (2022): Servirisma : Jurnal Pengabdian kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1311.061 KB) | DOI: 10.21460/servirisma.2022.21.10

Abstract

Brazilian spinach is a type of spinach that has not been widely consumed and cultivated by the Indonesian people even though it has excellent health benefits and is easy to cultivate. This Brazilian spinach cultivation training aims to empower the community during the pandemic by utilizing the land around the house or planting it in pots. The training participants were members of the GKI and GKJ churches from DIY and Central Java as well as some from Eastern Indonesia. Training participants are limited to 20 people with strict health protocols. The training materials are in the form of providing theory and direct practice in preparing seeds, media and planting as well as education about the benefits of brazil spinach for health. The results of this training were in the form of polybags containing spinach seeds that the participants had successfully harvested to take home and cultivate in their respective homes. Assistance for the Brazilian spinach cultivation process is carried out online through the WhatsApp group social media.
The Effect of Corn (Zea mays) Cob Extract on the Growth of Bifidobacteria Sejati, Ribka Ananda; Ariestanti, Catarina Aprilia; Amarantini, Charis
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 3 (2023): October 2023
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i3.6942

Abstract

Corncob is a source of dietary fiber, contains xylan polysaccharides (12.4%–31.94%), and is classified as the highest xylan-producing source compared to other agricultural wastes. Human digestive enzymes cannot degrade xylan but can be enzymatically degraded by lactic acid bacteria (LAB) through fermentation. This research aimed to evaluate the effect of corncob extract on the growth of Bifidobacterium. Corncob extract is obtained through alkaline extraction. The growth of Bifidobacterium bifidum FNCC 0462 and Bifidobacterium longum FNCC 0463 was observed through in vitro fermentation for 48 h using the total plate count (TPC) method. Bifidobacterium bifidum FNCC 0462 showed the ability to ferment corn cob extract for 48 h of fermentation with the highest growth at 8 h of fermentation (4,08 log10 CFU/mL) while B. longum FNCC 0463 was able to grow up to 16 h of fermentation only. The results indicated that corncob extract could support B. bifidum FNCC 0462 growth.  
Aktivitas Antibakteri Ekstrak Temulawak (Curcuma xanthorrhiza) dan Bakteriosin Bifidobacterium longum terhadap Salmonella typhi Prakasita, Vinsa Cantya; Ilham, Jessika; Amarantini, Charis
SCISCITATIO Vol. 5 No. 1 (2024): Volume 5 Number 1, January 2024
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2024.51.147

Abstract

Demam tifoid merupakan penyakit infeksi yang disebabkan oleh Salmonella typhi yang menyerang sistem pencernaan. Infeksi Salmonella ini menyebabkan morbiditas dan mortalitas yang signifikan di seluruh dunia. Penyakit ini dapat diatasi dengan antibiotik, namun saat ini sudah banyak terjadi resistensi antibiotik sehingga penggunaannya kurang efektif. Temulawak merupakan salah satu tanaman yang digunakan sebagai obat tradisional di Indonesia yang memiliki banyak manfaat kesehatan. Bacteriocin memiliki aktivitas antimikroba terhadap bakteri patogen. Oleh karena itu, penelitian ini bertujuan untuk mengetahui aktivitas daya hambat ekstrak etanol temulawak dan bakteriosin Bifidobacterium longum terhadap pertumbuhan S. typhi. Ekstraksi temulawak dilakukan dengan metode maserasi dengan pelarut etanol. Skrining fitokimia dengan berbagai reagen dilakukan untuk mengetahui senyawa yang ada di dalamnya. Kadar total fenolik diukur dengan menggunakan persamaan regresi yang dibandingkan dengan asam galat. Bakteriosin B. longum diperoleh dengan cara menambahkan NaOH pada supernatan hasil panen dan memanaskannya pada suhu tinggi. Uji aktivitas daya hambat ekstrak etanol temulawak (EET) dan bakteriosin B. longum (BBl) dilakukan dengan metode difusi disk. EET mengandung alkaloid, saponin, terpenoid, flavonoid, dan fenol dengan kadar total fenol 30,73 ± 2,81 mg GEA/g. EET memiliki aktivitas daya hambat yang rendah hingga sedang, sedangkan BBl memiliki aktivitas daya hambat yang rendah terhadap S. typhi.
Antibacterial Test of Kedondong Hutan Leaf (Spondias pinnata) Against Salmonella typhi Rangga, Febby Alvanda; Amarantini, Charis; Budiarso, Tri Yahya
Jurnal ILMU DASAR Vol 25 No 1 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i1.34622

Abstract

Kedondong hutan (Spondias pinnata) is a plant belonging to the Anacardiaceae family, plant that grows a lot in the Sulawesi area, one of which is in the Poso area, Central Sulawesi. The surrounding community uses this plant as a traditional food preparation which is often called the "Arogo Onco" by the community. Kedondong hutan contains active compounds that have the potential as antibacterial, namely flavonoids, saponins and tannins. The purpose of this study was to determine the potential of forest kedondong leaves in inhibiting the growth of Salmonella typhi bacteria. The extraction process was done the decoctation method using aquadest and while the antibacterial test was done using the diffusion method against three bacterial strains namely Salmonella typhi BPE 127.1 MC, BPE 122.4 CCA and NCTC 786. The results showed that the leaf extract of kedondong hutan (Spondias pinnata) contained flavonoids, saponins and tannins with the results of the analysis of total flavonoids 1514,9 mgQE/g, saponins 0.613% and tannins 8,94 mgTA/g. The results of the antibacterial test showed the greatest inhibitory power at a concentration of 100%. Salmonella typhi BPE 127.1 MC, BPE 122.4 CCA, NCTC 786, was the inhibition zone 17.67±0.58 mm, 15.67±0.58 mm and 20.33±0.58 mm were included in the category of strong inhibition zone.
The Effect of Addition Purple Sweet Potato Extract (Ipomea batatas L.) and Storage at 4oC on the Antioxidant and Antibacterial Activities of Yogurt Pare, Regina Londong; Budiarso, Tri Yahya; Amarantini, Charis
BIOEDUKASI Vol 22 No. 3 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i3.48470

Abstract

Yogurt is one of the fermented dairy products produced by lactic acid bacteria from milk, which contains beneficial nutritional components for human health. The innovation of yogurt production with the addition of probiotic cultures and food ingredients containing bioactive compounds can enhance its quality. This research aims to investigate the effect of adding purple sweet potato extract and storage treatment at 4℃ on yogurt's antioxidant and antibacterial activities. Four treatments were conducted in yogurt production, namely without adding purple sweet potato extract and adding 5%, 10%, and 15% (v/v) extract. All treatments were fermented using Lactobacillus bulgaricus, Streptococcus thermophilus, and Lactobacillus plantarum S1K2T1 as starter cultures. Subsequently, the yogurt was stored at 4℃ for 6 days, and chemical parameters, antioxidant, and antibacterial activities were examined. The highest result was observed in the treatment with 15% addition, showing a total antioxidant activity of 70.98% and an IC50 value of 13.96 ppm, which is equivalent to 2.06 times that of 200 ppm ascorbic acid. The six days storage affected antioxidant activity, but it was not significantly different. The antibacterial test showed indications of inhibition against Staphylococcus aureus ATCC 25923, Salmonella typhi NCTC 786, and Escherichia coli ATCC 25922, although the effect was very weak.
Pengelolaan Pencatatan Keuangan Gereja Berbasis Komputer di Gereja Kristen Jawa Medari, Sleman, Yogyakarta Oslan, Yetli; Amarantini, Charis; Santosa, Raden Gunawan; Ernawati, Lussy
Jurnal Abdi Masyarakat Indonesia Vol 4 No 6 (2024): JAMSI - November 2024
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jamsi.1463

Abstract

GKJ Medari adalah salah satu gereja yang masuk dalam klasis Yogyakarta Barat, sinode Jawa Tengah. GKJ Medari memiliki 5 (lima) wilayah pelayanan, yaitu GKJ Medari Murangan sebagai gereja induk, Pepanthan Cebongan, Pepanthan Tempel, Pepanthan Mancasan, dan Pepanthan Japanan. Sebelum PkM ini, pencatatan keuangan gereja di GKJ Medari dilakukan dengan menggunakan Microsoft Excel. Permasalahan yang muncul dari cara ini adalah pelaporan yang tidak dapat disajikan dengan tepat waktu, serta perbedaan cara pencatatan setiap wilayah pelayanan yang disebabkan karena belum digunakannya kode akun yang sesuai standar akuntansi. PkM ini merupakan upaya untuk meningkatkan kualitas pencatatan keuangan dengan membuat dan mengimplementasikan sebuah sistem keuangan berbasis komputer sesuai standar akuntansi untuk menghasilkan pencatatan keuangan yang transparan dan akuntabel. Tahapan yang digunakan pada PkM ini adalah observasi awal, perumusan masalah, pelaksanaan mencakup penyusuan CoA dan pembuatan sistem, implementasi, dan evaluasi. Hasil kegiatan PkM ini adalah daftar kode akun dengan struktur 6 (enam) digit sudah mengakomodasi keberagaman transaksi keuangan di GKJ Medari dan sebuah sistem informasi pengelolaan pencatatan keuangan. Keberadaan sistem ini juga telah mendorong terjadinya perubahan kecepatan dan ketepatan dalam pencatatan keuangan. Para pengelola keuangan juga sudah mulai memahami prinsip-prinsip akuntansi dalam pencatatan keuangan.
Identification of Lactic Acid Bacteria Inhibiting the Growth of Saccharomyces cerevisiae in Molasses Fermentation Vico, Yoel; Budiarso, Tri Yahya; Amarantini, Charis; Setyaningsih, Ratna
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 1 (2025): Inpress April 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i1.14168

Abstract

Ethanol production by Saccharomyces cerevisiae is essential for the biofuel industry, but microbial contamination by Lactic Acid Bacteria (LAB) can significantly reduce yield and fermentation efficiency. This research aimed to identify Lactic Acid Bacteria (LAB) causing significant inhibition of Saccharomyces cerevisiae growth. LAB isolates were screened and identification was carried out by carbohydrate fermentation using the 50 CHL Analytical Profile Index (API) Kit, and the LAB isolates (MC2K1, MP2K1, and MP2K2) were identified as Lactobacillus rhamnosus and isolate MC2K2 as L. pentosus. The results demonstrated that bacteriocins did not significantly inhibit S. cerevisiae following heat treatment, indicating that metabolic byproducts, particularly lactic acid, were primarily responsible for yeast inhibition. Co-culture of LAB and S. cerevisiae supernatants was used to determine inhibition by metabolites produced by LAB. A higher inhibitory effect occurred in the supernatant from 48 hours of incubation with lower CFU results. The highest inhibition rate reached 42% in isolate MC2K1 and the incubation time of 6 hours with all isolates significantly inhibited the growth of S. cerevisiae. These findings challenge the prevailing assumption that bacteriocins are the primary inhibitors of yeast and highlight the importance of metabolic byproducts as dominant inhibitory factors. This insight contributes to developing targeted contamination control strategies, ultimately enhancing ethanol production efficiency.
Analysis of microbiological hazards in turmeric tamarind traditional drink products Lifiana, Aulia; Amarantini, Charis; Budiarso, Tri Yahya
Jurnal Penelitian Saintek Vol 29, No 1 (2024)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i1.70596

Abstract

This study aimed to determine the presence of bacterial contaminants, specifically Escherichia coli, Salmonella sp., and Staphylococcus aureus, in turmeric tamarind traditional drink products according to the Indonesian National Standard (SNI 2019). The research was conducted using samples from 11 different locations in Yogyakarta. The samples were analyzed using Chromocult Coliform Agar (CCA), Salmonella Shigella Agar (SSA), and Baird Parker Agar (BPA) to isolate the specific bacteria. Further biochemical tests, including IMViC, Urease, and sugar fermentation tests, as well as confirmation using API 20E and API STAPH tests, were performed on the suspected isolates. The findings reveal that Escherichia coli, Salmonella sp., and Staphylococcus aureus were not detected in any of the samples. Thus, the traditional turmeric tamarind drink is considered safe for consumption according to the tested parameters. Keywords: turmeric tamarind, microbiological hazard, Klebsiella pneumoniae, Staphylococcus aureus. E. coli
Co-Authors -, Tri Wahyuni Sekar Sari Andriani, Eunike Marganingrum Ariestanti, Catarina Aprilia Ernawati, Lussy Ferdian, Evelyn Guruh Prihatmo Haripurnomo Kushadiwijaya Ilham, Jessika Langkah Sembiring Lifiana, Aulia Maria Trivonia Sema Pare, Regina Londong Pareakan, Jefyne Mali’ Raden Gunawan Santosa Rangga, Febby Alvanda RATNA SETYANINGSIH Samodra, Eunike Andriani Marganingrum Sejati, Ribka Ananda Sema, Maria Trivonia Suryanto, Regina TRI YAHYA BUDIARSO Vico, Yoel Vinsa Cantya Prakasita Wattimury, Angelia Widya Asmara Yetli Oslan