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All Journal Indian Journal of Forensic Medicine & Toxicology
David Guttery
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Public Health

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Analysis of MCL-1 Copy Number Variation in Non-Small Cell Lung Cancer Almahdi Alzaroukh Jaber; Howard Pringle; David Guttery; Aesa Alzaroug Jaber
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15579

Abstract

Amplification of the MCL-1 gene induces MCL-1 overexpression, which is an important marker for drug resistance in several malignancies through evasion of apoptosis. We analysed 39 lung adenocarcinomas (ADCs), 38 lung squamous cell carcinomas (SCCs), two large cell lung carcinomas (LCCs) and cell lines. A duplex ratio test TaqMan qPCR approach was performed for MCL-1 copy number change analysis and we demonstrated amplification of MCL-1 in H1395 cell line and copy number gain (isochromosome) in H2342 cell line. Immunohistochemistry (IHC) and in situ hybridization (ISH) were performed for protein and mRNA analysis respectively. MCL-1 amplification was detected in 13% and 2.5% of SCCs and ADCs respectively. MCL-1-amplified tumours demonstrated high expression of MCL-1 mRNA. Copy number gain was detected in 2.5 % of ADC but not SCC.
Co-Authors Aesa Alzaroug Jaber Almahdi Alzaroukh Jaber Howard Pringle