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Aron Batubara
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Agriculture, Biological Sciences & Forestry Veterinary

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Variasi Genetik Gen Myostatin Ekson 3 pada Sembilan Bangsa Kambing Lokal di Indonesia Rissa Herawati Ginting; Achmad Farajallah; Dyah Perwitasari Farajallah; Aron Batubara
Jurnal Ilmu Pertanian Indonesia Vol. 22 No. 2 (2017): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (523.264 KB) | DOI: 10.18343/jipi.22.2.73

Abstract

Myostatin gene plays a role in helping to control the growth and development of muscle tissue. Identification of genetic diversity in nine local goat breeds in Indonesia has done. The aim of this study was to obtain information myostatin gene diversity of exon 3 in local goats in Indonesia. The total of 10 samples was selected from 80 samples of goat's blood collected comprising each sample of the population of goats breeds, i.e., Samosir, Muara, Kacang, Costa, Peranakan Etawah, Boerawa, Gembrong, Boer, and Boerka goats. The gene diversity and nucleotide base changes were identified by using polymerase chain reaction (PCR) and sequencing techniques. The analysis showed that there is eight variant identified in appropriate with those found in sequencing results. Deletion variations were found in Costa and Samosir goats in T552- and G560-. Substitution variations were found in Gembrong (A7C & A11T), Peranakan Etawah (T10A & A11T), Burawa (T10A & A11T), Muara (A11T), Samosir (A11T), and Boerka (A182T, T437A, T439A, & A445G). Variations on the chromatogram peak overlapping contained in the base position to 13. Analysis of variance showed that there was a special mutation in exon 3 that affects the amino acid tyrosine into lysine. Variants were found in nine goat breeds associated with phylogenetic and genetic distance of goats, Boerka goat has the highest level of genetic variation, it indicated that Boerka goat was crossbreed
Molecular characterization of six sub population Indonesian local goats based on mitochondrial DNA D-loop Aron Batubara; R.R. Noor; A. Farajallah; B. Tiesnamurti; M. Doloksaribu
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 1 (2011): MARCH 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.805 KB) | DOI: 10.14334/jitv.v16i1.634

Abstract

Indonesian local goats were spread in some region, but there was still limited data’s known about the characteristics of its genetic diversity and origin. The Mitochondrial DNA D-loop sequences were used to study the genetic diversity and relationships of six sub population Indonesian local goats, namely, Kacang, Marica, Samosir, Jawarandu, Muara and Bengali goats. From 539 blood samples and DNA extraction collections were selected about 60 samples (10 samples each sub populations) analyzed by PCR-RFLP methods, followed sequence analyzed about 5 PCR products each sub population. The results of the sequence analyses were edited and acquired about 957 bp of nucleotides length. After the alignment analyses were found 50 polymorphic sites which divided into 19 haplotype groups of mtDNA D-loop region. The value of nucleotide diversity was 0.014 ± 0.002. Analysis of Neighbour Joining with Kimura 2 Parameter methods and bootstrap test with 1000 replication indicated that each sub population groups was significantly different between one groups to the others. The maternal lineages origin of six breeds of Indonesian local goats was included to the group of lineage B. The Lineage B was the maternal origin of the haplogroup of goats in the region of East Asia, South Asia, China, Mongolia, North and South Africa, Malaysia, Indonesia, Pakistan and India. Key words: Genetic Diversity, mtDNA D-loop, Haplotypes, Local Goats
Responses of two genotypes of lambs on the infection of Haemonchus contortus and the level of energy supplements Simon P Ginting; Aron Batubara; Endang Romjali; M Rangkuti; Subandriyo .
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 1 (1999): MARCH 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.216 KB) | DOI: 10.14334/jitv.v4i1.133

Abstract

A study was conducted to evaluate the responses of lambs on the infection of Haemonchus contortus and the level of energy supplements. Forty eight male lambs, 3-4 months old were used in a factorial experimental design (2x2x2) which lasted for 9 weeks. The factors were breed (Local Sumatra and Sungai Putih: 50% Local Sumatra, 25% St. Croix; 25% Barbados Blackbelly), level of Haemonchus infection (3,000 L3 and 1,500 L3 per week) and level of energy supplement (2,900 kcal/kg DM and 2,300 kcal/kg DM). Infection at 3,000 L3 decreased (P<0.05) ADG of lambs of both energy levels. An extremely low ADG (10-16 g) was observed in 3000 L3 group given low energy level. Body condition of lambs fed high energy level was constant over the experiment, but it decreased at the rate of 0.9 to 1.1 unit at the low energy supplement. EPG was higher (P>0.001) in the 3000 L3 group, was lower (P<0.05) in the Local Sumatra, but not affected (P>0.10) by energy level. PCV was not affected (P>0.10) by infection levels, but lower (P<0.01) in the low energy group or in the St Croix crosses. Eosinophil and serum protein concentration were not affected by energy and infection level, but they were higher (P<0.01) in the local Sumatra. It was concluded that strong effects of level of infection and genotype was detected to influence the impact of Haemonchus infection in lambs. However, the effect of energy levels was less consistent as judged from the EPG, PCV, eosinophil and serum protein data.   Key words : Energy level, genotype, infection level, Haemonchus contortus, lambs
Co-Authors ACHMAD FARAJALLAH B. Tiesnamurti DYAH PERWITASARI -FARAJALLAH Endang Romjali M Rangkuti M. Doloksaribu R.R. Noor Rissa Herawati Ginting Simon P Ginting Subandriyo .